Results 51 to 60 of about 19,171 (192)

Fluctuation spectroscopy of giant unilamellar vesicles using confocal and phase contrast microscopy [PDF]

open access: yesbioRxiv, 2020
A widely used method to measure the bending rigidity of bilayer membranes is fluctuation spectroscopy, which analyses the thermally-driven membrane undulations of giant unilamellar vesicles recorded with either phase-contrast or confocal microscopy. Here,
H. Faizi   +4 more
semanticscholar   +1 more source

Pulling Membrane Nanotubes from Giant Unilamellar Vesicles [PDF]

open access: yesJournal of Visualized Experiments, 2017
The reshaping of the cell membrane is an integral part of many cellular phenomena, such as endocytosis, trafficking, the formation of filopodia, etc. Many different proteins associate with curved membranes because of their ability to sense or induce membrane curvature.
Prévost, Coline   +3 more
openaire   +2 more sources

Generation of Giant Unilamellar Vesicles (GUVs) Using Polyacrylamide Gels

open access: yesBio-Protocol, 2020
Giant unilamellar vesicles (GUVs) are a widely used model system for a range of applications including membrane biophysics, drug delivery, and the study of actin dynamics.
Eric Parigoris   +2 more
doaj   +1 more source

Shape Transformations of Lipid Vesicles by Insertion of Bulky-Head Lipids. [PDF]

open access: yesPLoS ONE, 2015
Lipid vesicles, in particular Giant Unilamellar Vesicles (GUVs), have been increasingly important as compartments of artificial cells to reconstruct living cell-like systems in a bottom-up fashion.
Soichiro Tsuda   +4 more
doaj   +1 more source

Experimental platform for the functional investigation of membrane proteins in giant unilamellar vesicles

open access: yesbioRxiv, 2021
Giant unilamellar vesicles (GUVs) are micrometer-sized model membrane systems that can be viewed directly under the microscope. They serve as scaffolds for the bottom-up creation of synthetic cells, targeted drug delivery and have been used in many in ...
Nicolas Dolder   +2 more
semanticscholar   +1 more source

A Note on Vestigial Osmotic Pressure

open access: yesMembranes, 2023
Recent experiments have indicated that at least a part of the osmotic pressure across the giant unilamellar vesicle (GUV) membrane was balanced by the rapid formation of the monodisperse daughter vesicles inside the GUVs through an endocytosis-like ...
Hao Wu   +2 more
doaj   +1 more source

Endoplasmic reticulum phospholipid scramblase activity revealed after protein reconstitution into giant unilamellar vesicles containing a photostable lipid reporter

open access: yesScientific Reports, 2021
Transbilayer movement of phospholipids in biological membranes is mediated by a diverse set of lipid transporters. Among them are scramblases that facilitate a rapid bi-directional movement of lipids without metabolic energy input. Here, we established a
P. Mathiassen   +2 more
semanticscholar   +1 more source

Membrane sculpting by curved DNA origami scaffolds

open access: yesNature Communications, 2018
BAR domain proteins feature a “banana-like” shape which is thought to aid membrane scaffolding and membrane tubulation. Here authors use DNA origami mimicking BAR domains, giant unilamellar vesicles and fluorescence imaging to study how different BAR ...
Henri G. Franquelim   +4 more
doaj   +1 more source

Deciphering the Assembly of Enveloped Viruses Using Model Lipid Membranes

open access: yesMembranes, 2022
The cell plasma membrane is mainly composed of phospholipids, cholesterol and embedded proteins, presenting a complex interface with the environment.
Erwan Brémaud   +2 more
doaj   +1 more source

Light-Triggered Cargo Loading and Division of DNA-Containing Giant Unilamellar Lipid Vesicles

open access: yesNano letters (Print), 2021
A minimal synthetic cell should contain a substrate for information storage and have the capability to divide. Notable efforts were made to assemble functional synthetic cells from the bottom up, however often lacking the capability to reproduce.
Yannik Dreher   +3 more
semanticscholar   +1 more source

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