Results 51 to 60 of about 26,375 (226)

Electroformation of Giant Unilamellar Vesicles on Stainless Steel Electrodes [PDF]

ACS Omega, 2017
Giant unilamellar vesicles (GUVs) are well-established model systems for studying membrane structure and dynamics. Electroformation, also referred to as electroswelling, is one of the most prevalent methods for producing GUVs, as it enables modulation of the lipid hydration process to form relatively monodisperse, defect-free vesicles.
Valerio Pereno, Dario Carugo, Luca Bau, Erdinc Sezgin, Jorge Bernardino de la Serna, Christian Eggeling, Eleanor Stride   +6 more
doaj   +9 more sources

Formation of unilamellar dipalmitoylphosphatidylcholine vesicles promoted by Ca2+ ions: A small-angle neutron scattering study [PDF]

, 2007
Dipalmitoylphosphatidylcholine (DPPC) was hydrated in 0.2-60 mM solution of CaCl 2 in heavy water and thoroughly homogenized by freezing-thawing process.
Almásy, László, Lengyel, Adrián, Teixeira, José, Uhríková, Daniela   +3 more
core   +1 more source

Actin crosslinker competition and sorting drive emergent GUV size-dependent actin network architecture

Communications Biology, 2021
By encapsulating proteins in giant unilamellar vesicles, Bashirzadeh et al find that actin crosslinkers, α-actinin and fascin, can self-assemble with actin into complex structures that depend on the degree of confinement.
Yashar Bashirzadeh, Steven A. Redford, Chatipat Lorpaiboon, Alessandro Groaz, Hossein Moghimianavval, Thomas Litschel, Petra Schwille, Glen M. Hocky, Aaron R. Dinner, Allen P. Liu   +9 more
doaj   +1 more source

Using small molecules to facilitate exchange of bicarbonate and chloride anions across liposomal membranes [PDF]

, 2009
Bicarbonate is involved in a wide range of biological processes, which include respiration, regulation of intracellular pH and fertilization. In this study we use a combination of NMR spectroscopy and ion-selective electrode techniques to show that the ...
A Fürstner, AP Davis, AV Koulov, C Yamamoto, D Stefayne, E Gout, ED Steinle, F Hofmeister, FA Norris, FG Riddell, HG Hempling, HM Florman, J Fujinaga, Jeffery T. Davis, JL Sessler, Jose Carlos Iglesias-Sánchez, JR Casey, JW Bennett, JY Choi, K Chang, K Kavallieratos, K Tanigaki, L You, M Nguyen, MS Melvin, NM Koropatkin, NN Gerber, NR Williamson, OA Okunola, Oluyomi A. Okunola, Philip A. Gale, Pilar Prados, PV Santacroce, RA Gottlieb, Roberto Quesada, S Ohkuma, T Sato, Tomás Torroba, V Rizzo, WN Lipscomb, WW Cleland, X Li   +41 more
core   +1 more source

Fluctuation spectroscopy of giant unilamellar vesicles using confocal and phase contrast microscopy [PDF]

bioRxiv, 2020
A widely used method to measure the bending rigidity of bilayer membranes is fluctuation spectroscopy, which analyses the thermally-driven membrane undulations of giant unilamellar vesicles recorded with either phase-contrast or confocal microscopy. Here,
H. Faizi, Cody J. Reeves, V. Georgiev, Petia M. Vlahovska, R. Dimova   +4 more
semanticscholar   +1 more source

On-chip inverted emulsion method for fast giant vesicle production, handling, and analysis

, 2020
Liposomes and giant unilamellar vesicles (GUVs) in particular are excellent compartments for constructing artificial cells. Traditionally, their use requires bench-top vesicle growth, followed by experimentation under a microscope.
Robinson, T., Seemann, T., Yandrapalli, N.   +2 more
core   +1 more source

Selective flow-induced vesicle rupture to sort by membrane mechanical properties [PDF]

, 2015
International audienceVesicle and cell rupture caused by large viscous stresses in ultrasonication is central to biomedical and bioprocessing applications.
Brooks, NJ, Garbin, V, Pommella, A, Seddon, JM   +3 more
core   +2 more sources

Active Membrane Fluctuations Studied by Micropipet Aspiration [PDF]

, 2001
We present a detailed analysis of the micropipet experiments recently reported in J-B. Manneville et al., Phys. Rev. Lett. 82, 4356--4359 (1999), including a derivation of the expected behaviour of the membrane tension as a function of the areal strain ...
A. N. Radionov, A. Royant, A.G. Petrov, D. Oesterhelt, E. Boroske, E. Evans, E. Evans, E. Pebay-Peyroula, F. Dumas, F. Faucon, F. Maingret, F. Maingret, G. Niggemann, H. J. Sass, H. Luecke, H. Luecke, J-B. Manneville, J. Heberle, J. Heberle, J. K. Lanyi, J. Prost, J. Prost, J. Prost, J.-B. Manneville, J.-L. Rigaud, M. Bloom, M. Bloom, M. I. Angelova, M. Jansen, M. Kummrow, M. M. Speretto, M. Seigneuret, M. Seigneuret, N. A. Dencher, O. G. Mouritsen, P. Bassereau, P. Méléard, Ph. Méléard, R. Henderson, R. Kwok, R. Lawaczeck, R. Lipowsky, S. Ramaswamy, S. Ramaswamy, S. Ramaswamy, S. Subramaniam, S. Subramaniam, T. Kouyama, U. Haupts, W. Helfrich, W. Kuhlbrandt, Y. Cao, Y. Kimura   +52 more
core   +2 more sources

Endoplasmic reticulum phospholipid scramblase activity revealed after protein reconstitution into giant unilamellar vesicles containing a photostable lipid reporter

Scientific Reports, 2021
Transbilayer movement of phospholipids in biological membranes is mediated by a diverse set of lipid transporters. Among them are scramblases that facilitate a rapid bi-directional movement of lipids without metabolic energy input. Here, we established a
P. Mathiassen, A. Menon, T. Günther Pomorski   +2 more
semanticscholar   +1 more source

Construction of membrane-bound artificial cells using microfluidics: a new frontier in bottom-up synthetic biology [PDF]

, 2016
The quest to construct artificial cells from the bottom-up using simple building blocks has received much attention over recent decades and is one of the grand challenges in synthetic biology.
Elani, Y
core   +2 more sources