Femtoliter Injection of ESCRT-III Proteins into Adhered Giant Unilamellar Vesicles. [PDF]
Bio Protoc, 2022 The endosomal sorting complex required for transport (ESCRT) machinery mediates membrane fission reactions that exhibit a different topology from that observed in clathrin-coated vesicles.
Georgiev VN +3 more
europepmc +2 more sources
Pulling Membrane Nanotubes from Giant Unilamellar Vesicles [PDF]
Journal of Visualized Experiments, 2017 The reshaping of the cell membrane is an integral part of many cellular phenomena, such as endocytosis, trafficking, the formation of filopodia, etc. Many different proteins associate with curved membranes because of their ability to sense or induce membrane curvature.
Prévost, Coline +3 more
openaire +2 more sources
Generation of Giant Unilamellar Vesicles (GUVs) Using Polyacrylamide Gels
Bio-Protocol, 2020 Giant unilamellar vesicles (GUVs) are a widely used model system for a range of applications including membrane biophysics, drug delivery, and the study of actin dynamics.
Eric Parigoris +2 more
doaj +1 more source
Shape Transformations of Lipid Vesicles by Insertion of Bulky-Head Lipids. [PDF]
PLoS ONE, 2015 Lipid vesicles, in particular Giant Unilamellar Vesicles (GUVs), have been increasingly important as compartments of artificial cells to reconstruct living cell-like systems in a bottom-up fashion.
Soichiro Tsuda +4 more
doaj +1 more source
Imaging of photoacoustic-mediated permeabilization of giant unilamellar vesicles (GUVs)
Scientific Reports, 2021 Target delivery of large foreign materials to cells requires transient permeabilization of the cell membrane without toxicity. Giant unilamellar vesicles (GUVs) mimic the phospholipid bilayer of the cell membrane and are also useful drug delivery ...
Diogo A. Pereira +9 more
doaj +1 more source
A Note on Vestigial Osmotic Pressure
Membranes, 2023 Recent experiments have indicated that at least a part of the osmotic pressure across the giant unilamellar vesicle (GUV) membrane was balanced by the rapid formation of the monodisperse daughter vesicles inside the GUVs through an endocytosis-like ...
Hao Wu +2 more
doaj +1 more source
Membrane sculpting by curved DNA origami scaffolds
Nature Communications, 2018 BAR domain proteins feature a “banana-like” shape which is thought to aid membrane scaffolding and membrane tubulation. Here authors use DNA origami mimicking BAR domains, giant unilamellar vesicles and fluorescence imaging to study how different BAR ...
Henri G. Franquelim +4 more
doaj +1 more source
Microfluidic production, stability and loading of synthetic giant unilamellar vesicles. [PDF]
Sci RepIn advanced drug delivery, versatile liposomal formulations are commonly employed for safer and more accurate therapies. Here we report a method that allows a straightforward production of synthetic monodisperse (~ 100 μm) giant unilamellar vesicles ...
Ernits M +11 more
europepmc +2 more sources
Deciphering the Assembly of Enveloped Viruses Using Model Lipid Membranes
Membranes, 2022 The cell plasma membrane is mainly composed of phospholipids, cholesterol and embedded proteins, presenting a complex interface with the environment.
Erwan Brémaud +2 more
doaj +1 more source
Photoactivated Signaling Networks using DNA‐Based Synthetic Organelles as Biomimetic Protocells
Angewandte Chemie, Volume 138, Issue 25, 15 June 2026.Light‐triggered intercommunication between two types of organelle‐loaded liposomes lead to DNAzyme‐mediated on transcription‐guided structural reconfiguration of the organelles. ABSTRACT Membraneless organelles formed by phase‐separated nucleic acid or protein condensates play vital roles in regulating cellular functions.
Huiying Xue +6 more
wiley +2 more sources