Results 71 to 80 of about 6,262 (195)

Electroformation of Giant Unilamellar Vesicles from Damp Lipid Films with a Focus on Vesicles with High Cholesterol Content. [PDF]

Membranes (Basel)
Giant unilamellar vesicles (GUVs) are membrane models used to study membrane properties. Electroformation is one of the methods used to produce GUVs. During electroformation protocol, dry lipid film is formed.
Mardešić I, Boban Z, Raguz M.
europepmc   +2 more sources

Bottom‐Up Coacervate‐Based Artificial Cells: Integrating Cellular Hallmarks into Complex Life‐Like Systems

Angewandte Chemie, Volume 138, Issue 22, 25 May 2026.
Current interest in artificial cell research underscores its potential to deepen our understanding of life's fundamental processes. This review highlights advances in bottom‐up coacervate‐based artificial cell engineering via combined integration of cellular hallmarks.
Arjan Hazegh Nikroo, Angshuman Das, Madelief A. M. Verwiel, Jan C. M. van Hest   +3 more
wiley   +2 more sources

Efficacy Assessment of Montanide GEL 02 PR and Liposome‐Based Encapsulation as Adjuvants for an FrpA Subunit Vaccine Against Photobacteriosis in Solea senegalensis

Journal of Fish Diseases, EarlyView.
ABSTRACT Photobacteriosis is a major threat to marine aquaculture, and the siderophore transporter FrpA represents a promising target for effective subunit vaccine development. In this study, we evaluated Montanide GEL 02 PR and phosphatidylcholine‐based liposomes as aqueous and safer alternatives to Freund's adjuvant for an FrpA‐based subunit vaccine ...
Marta A. Lages, Miguel Balado, Abel M. Forero, Lucía Ageitos, Jaime Rodríguez, Carlos Jiménez, Manuel L. Lemos, Beatriz Magariños   +7 more
wiley   +1 more source

Functional Reconstitution of a Voltage-Gated Potassium Channel in Giant Unilamellar Vesicles [PDF]

PLoS ONE, 2011
Voltage-gated ion channels are key players in cellular excitability. Recent studies suggest that their behavior can depend strongly on the membrane lipid composition and physical state. In vivo studies of membrane/channel and channel/channel interactions
Sophie Aimon, John Manzi, Daniel Schmidt, José A. Poveda, Patricia Bassereau, Gilman E. S. Toombes   +5 more
openalex   +3 more sources

Diphtheria toxin T-domain as a tool for inducing lipid vesicle fusion

Communications Chemistry
Membrane fusion is fundamental for many biological processes, including subcellular compartmentalization, cell growth, and exo- and endocytosis. It also plays a key role in the fabrication of artificial membrane structures, particularly vesicles. However,
Piotr Jasko, Maryame Bina, Daniel Frey, Cora-Ann Schoenenberger, Cornelia G. Palivan, Richard A. Kammerer   +5 more
doaj   +1 more source

A membrane filtering method for the purification of giant unilamellar vesicles

Chemistry and Physics of Lipids, 2011
The use of giant unilamellar vesicles (GUVs) for investigating the properties of biomembranes is advantageous compared to the use of small-sized vesicles such as large unilamellar vesicles (LUVs). Experimental methods using GUVs, such as the single GUV method, would benefit if there was a methodology for obtaining a large population of similar-sized ...
Yukihiro, Tamba, Hiroaki, Terashima, Masahito, Yamazaki   +2 more
openaire   +2 more sources

High-speed imaging of giant unilamellar vesicle formation in cDICE

ACS Omega, 2023
Abstract Giant unilamellar vesicles (GUVs) are widely used as in vitro model membranes in biophysics and as cell-sized containers in synthetic biology. Despite their ubiquitous use, there is no one-size-fits-all method for their production.
Lori Van de Cauter, Yash K. Jawale, Daniel Tam, Lucia Baldauf, Lennard van Buren, Gijsje H. Koenderink, Marileen Dogterom, Kristina A. Ganzinger   +7 more
openaire   +4 more sources

Membrane Disintegration Caused by the Steroid Saponin Digitonin Is Related to the Presence of Cholesterol

Molecules, 2015
In the present investigation we studied the molecular mechanisms of the monodesmosidic saponin digitonin on natural and artificial membranes. We measured the hemolytic activity of digitonin on red blood cells (RBCs). Also different lipid membrane models (
Ikhwan Resmala Sudji, Yamunadevi Subburaj, Nataliya Frenkel, Ana J. García-Sáez, Michael Wink   +4 more
doaj   +1 more source

Mimicking a Light‐Harvesting Complex to Accelerate Photooxidation in Asymmetric Lipid Membrane Nanoreactors

Angewandte Chemie International Edition, Volume 65, Issue 25, 15 June 2026.
Biomimetic liposome nanoreactors mimicking natural light‐harvesting complexes enable efficient photooxidation of NADH. Membrane‐anchored fluorescein transfers energy via FRET to membrane anchored eosin Y, oxidizing NADH. While symmetric membranes show little improvement, asymmetric chromophore distribution enhances photooxidation rates by up to 27 ...
Julian Bösking, Roland E. P. Nau, Nico Alleva, Richard Jacobi, Tobias Meyer‐Zedler, Hannah Voßhenrich, Francesca Mazotta, Ingo Lieberwirth, David Ng, Michael Schmitt, Jürgen Popp, Leticia González, Tanja Weil, Andrea Pannwitz   +13 more
wiley   +1 more source

Encapsulation of protein/DNA complexes into unilamellar liposomes via annexin-mediated membrane recruitment and sonication

Cell Reports: Methods
Summary: This paper reports an effective protocol to encapsulate native protein/DNA complexes into unilamellar vesicles composed of natural lipids without the use of organic solvents, in physiological buffers, and at low protein/DNA concentrations.
Michael Burger, Finn Brigger, Valeria Mantella, Jean-Christophe Leroux   +3 more
doaj   +1 more source