Results 81 to 90 of about 19,171 (192)

Tuning Membrane Binding and Lipid Phase Preference of DNA Nanostructures via Synergistic Multi‐Lipidation

ChemSystemsChem, Volume 8, Issue 5, September 2026.
DNA origami nanostructures functionalized with lipophilic anchors of distinct hydrophobicity reveal synergistic effects on membrane binding and phase‐specific localization. Weak anchors, although insufficient for binding alone, enhance membrane association and govern selective phase partitioning when combined with strong anchors, establishing multi ...
Subhasini Singh, Henri G. Franquelim
wiley   +1 more source

A membrane filtering method for the purification of giant unilamellar vesicles

Chemistry and Physics of Lipids, 2011
The use of giant unilamellar vesicles (GUVs) for investigating the properties of biomembranes is advantageous compared to the use of small-sized vesicles such as large unilamellar vesicles (LUVs). Experimental methods using GUVs, such as the single GUV method, would benefit if there was a methodology for obtaining a large population of similar-sized ...
Yukihiro, Tamba, Hiroaki, Terashima, Masahito, Yamazaki   +2 more
openaire   +2 more sources

High-speed imaging of giant unilamellar vesicle formation in cDICE

ACS Omega, 2023
Abstract Giant unilamellar vesicles (GUVs) are widely used as in vitro model membranes in biophysics and as cell-sized containers in synthetic biology. Despite their ubiquitous use, there is no one-size-fits-all method for their production.
Lori Van de Cauter, Yash K. Jawale, Daniel Tam, Lucia Baldauf, Lennard van Buren, Gijsje H. Koenderink, Marileen Dogterom, Kristina A. Ganzinger   +7 more
openaire   +4 more sources

Encapsulation of protein/DNA complexes into unilamellar liposomes via annexin-mediated membrane recruitment and sonication

Cell Reports: Methods
Summary: This paper reports an effective protocol to encapsulate native protein/DNA complexes into unilamellar vesicles composed of natural lipids without the use of organic solvents, in physiological buffers, and at low protein/DNA concentrations.
Michael Burger, Finn Brigger, Valeria Mantella, Jean-Christophe Leroux   +3 more
doaj   +1 more source

Visualization and analysis of apolipoprotein A-I interaction with binary phospholipid bilayers

Journal of Lipid Research, 2005
Apolipoprotein A-I (apoA-I) interaction with specific cell lipid domains was suggested to trigger cholesterol and phospholipid efflux. We analyzed here apoA-I interaction with dimyristoylphosphatidylcholine/distearoylphosphatidylcholine (DMPC/DSPC ...
M. Alejandra Tricerri, Juan D. Toledo, Susana A. Sanchez, Theodore L. Hazlett, Enrico Gratton, Ana Jonas, Horacio A. Garda   +6 more
doaj   +1 more source

Effect of the Membrane Composition of Giant Unilamellar Vesicles on Their Budding Probability: A Trade-Off between Elasticity and Preferred Area Difference

Life, 2021
The budding and division of artificial cells engineered from vesicles and droplets have gained much attention in the past few decades due to an increased interest in designing stimuli-responsive synthetic systems.
Ylenia Miele, Gábor Holló, István Lagzi, Federico Rossi   +3 more
doaj   +1 more source

Environmental Impact of Synthetic Cell Technology: Review of Life Cycle Assessment Data for Feedstocks and Production Methods

ChemBioChem, Volume 27, Issue 15, 14 August 2026.
Synthetic cells are positioned as enablers of green technology. But what is known about the environmental impact of synthetic cell production? In this review, we examine drivers of greenhouse gas emissions, toxicity, and other environmental impact categories of synthetic cell components.
Christina Hopf, Mehdi Ravandeh, Jan Steinkühler   +2 more
wiley   +1 more source

Repurposing a Lipid Droplet‐Associated Algal Protein as a Biosensor for Phosphatidic Acid

Advanced Sensor Research, Volume 5, Issue 8, August 2026.
The DTH1 lipid binding domain (DTH1LBD) binds to lipid membranes containing phosphatidic acid (PA). Its N‐terminal amphipathic helices are sufficient for this association and, when labeled with solvatochromic fluorophores, can serve as in vitro PA biosensors.
Bilge Ercan, Nian Kee Tan, Julia Lin, Jingyao You, Stephen M. Butler, Katrina A. Jolliffe   +5 more
wiley   +1 more source

Visualization and Quantification of Transmembrane Ion Transport into Giant Unilamellar Vesicles [PDF]

Angewandte Chemie International Edition, 2014
AbstractTransmembrane ion transporters (ionophores) are widely investigated as supramolecular agents with potential for biological activity. Tests are usually performed in synthetic membranes that are assembled into large unilamellar vesicles (LUVs). However transport must be followed through bulk properties of the vesicle suspension, because LUVs are ...
Valkenier, H., López Mora, N.F., Kros, A., Davis, A.P.   +3 more
openaire   +6 more sources

Light‐Activated Communication Between Synthetic and Living Cells via Photoresponsive Membranes

ChemBioChem, Volume 27, Issue 14, 29 July 2026.
Light‐triggered membrane rupture of SCs enables controlled release of adenosine triphosphate (ATP) and histamine, which activate purinergic and histaminergic signaling in HeLa cells, leading to intracellular Ca2+ mobilization. This biomimetic communication platform provides a simple strategy for engineering remotely controlled chemical signaling ...
Roberto Barbaro, Paola Albanese, Matilde Colella, Davide Lattarulo, Samrat Saha, Jack L.‐Y. Chen, Fabio Mavelli, Federico Rossi, Emiliano Altamura   +8 more
wiley   +1 more source