Results 81 to 90 of about 26,375 (226)

Method for immobilization of living and synthetic cells for high-resolution imaging and single-particle tracking

Scientific Reports, 2018
Super-resolution imaging and single-particle tracking require cells to be immobile as any movement reduces the resolution of the measurements. Here, we present a method based on APTES-glutaraldehyde coating of glass surfaces to immobilize cells without ...
Łukasz Syga, Dian Spakman, Christiaan M. Punter, Bert Poolman   +3 more
doaj   +1 more source

Shaping Giant Membrane Vesicles in 3D-Printed Protein Hydrogel Cages

, 2020
Giant unilamellar phospholipid vesicles are attractive starting points for constructing minimal living cells from the bottom-up. Their membranes are compatible with many physiologically functional modules and act as selective barriers, while retaining a ...
Eto, H., Franquelim, H., Heymann, M., Jia, H., Litschel, T., Schwille, P.   +5 more
core   +1 more source

Charge-induced phase separation in lipid membranes [PDF]

, 2014
The phase separation in lipid bilayers that include negatively charged lipids is examined experimentally. We observed phase-separated structures and determined the membrane miscibility temperatures in several binary and ternary lipid mixtures of ...
Andelman, David, Hamada, Tsutomu, Himeno, Hiroki, Komura, Shigeyuki, Shimokawa, Naofumi, Takagi, Masahiro   +5 more
core   +2 more sources

Low-cost equipment for electroformation of Giant Unilamellar Vesicles

HardwareX, 2018
Giant Unilamellar Vesicles (GUVs) constitute a very useful model for biomembranes. They are usually generated by electroformation using sinusoidal potential waves applied between two platinum electrodes or electrodes composed of glasses covered by indium
Javier Alejandro Bellon, Marcelo Javier Pino, Natalia Wilke   +2 more
doaj   +1 more source

Studying Macromolecular Composition in Cell–Cell Interfaces Using 3D Membrane Reconstitution Systems

Advanced Science, EarlyView.
A comprehensive understanding of the interactions between proteins, lipids and glycocalyx components at the immune synapse is still lacking. Here, an artificial and a semi‐artificial model contact system were established to reconstitute the cell‐cell contact in 3D. The model systems enable the examination of macromolecule enrichment or depletion at the
Franziska Ragaller, Amelie Maribel Schneider, Ellen Sjule, Renhua Sun, Xiao Han, Luca Andronico, Edward Jenkins, Michael Dustin, Adnane Achour, Erdinc Sezgin   +9 more
wiley   +1 more source

Heat-induced formation of single giant unilamellar vesicles [PDF]

, 2011
Giant unilamellar vesicles (GUVs) are an excellent model system for the investigation of lipid membranes, the study of membrane proteins and ion channels in a biomimetic environment, and in the creation of artificial cells.
Billerit, Celine, Dommersnes, Paul, Jeffries, Gavin, Jesorka, Aldo, Orwar, Owe, Wegrzyn, Ilona   +5 more
core   +1 more source

Reconstitution of proteins on electroformed giant unilamellar vesicles [PDF]

, 2015
In vitro reconstitution of simplified biological systems from molecular parts has proven to be a powerful method for investigating the biochemical and biophysical principles underlying cellular processes. In recent years, there has been a growing interest in reconstitution of protein-membrane interactions to understand the critical role played by ...
Schmid, E., Richmond, D., Fletcher, D.
openaire   +3 more sources

Lipid Lateral Organization on Giant Unilamellar Vesicles Containing Lipopolysaccharides [PDF]

Biophysical Journal, 2011
We developed a new (to our knowledge) protocol to generate giant unilamellar vesicles (GUVs) composed of mixtures of single lipopolysaccharide (LPS) species and Escherichia coli polar lipid extracts. Four different LPSs that differed in the size of the polar headgroup (i.e., LPS smooth > LPS-Ra > LPS-Rc > LPS-Rd) were selected to generate GUVs composed
Kubiak, Jakub, Brewer, Jonathan R., Hansen, Søren, Bagatolli, Luis   +3 more
openaire   +4 more sources

Membranes by the Numbers [PDF]

, 2017
Many of the most important processes in cells take place on and across membranes. With the rise of an impressive array of powerful quantitative methods for characterizing these membranes, it is an opportune time to reflect on the structure and function ...
A Finkelstein, A Flamholz, A Nenninger, A Pockels, A Schmidt, A Upadhyaya, AD Dupuy, AE Shyer, AF Bitbol, C Klose, C Klose, C Nielsen, C Tanford, CS Ejsing, D Boal, D Chow, E Evans, E Gorter, E Layre, E Richardson, ES Haswell, FC Neidhardt, FM Hochmuth, G Meer van, GS Manning, GW Li, H Fricke, HC Gaede, HG Garcia, HJ Curtis, I Langmuir, I Vorobyov, J Dai, J Peukes, J Schlessinger, J Song, JB Dacks, JF Nagle, JF Nagle, JF Nagle, JL Sampaio, JT Mika, KC Olbrich, KR Levental, L Bintu, L Fagerberg, L Kalvodova, LK Buehler, M Bialecka-Fornal, M Carvalho, M Kumar, M Li, M Louhivuori, Maya Shamir, MC Field, MK Doeven, MR Alecio, O Kedem, OS Andersen, P Sens, PF Fahey, PG Saffman, R Milo, R Milo, R Phillips, R Phillips, R Phillips, RW Robinett, S Mahajan, S Ramadurai, S Takamori, T Hanai, T Savin, TA Harroun, U Seifert, Uri Moran, W Almers, W Bialek, W Rawicz, Walter Neupert, WD Stein, Y Gambin, Y Shibata, Y Shibata   +83 more
core   +2 more sources

Functional reconstitution of a voltage-gated potassium channel in giant unilamellar vesicles. [PDF]

PLoS ONE, 2011
Voltage-gated ion channels are key players in cellular excitability. Recent studies suggest that their behavior can depend strongly on the membrane lipid composition and physical state. In vivo studies of membrane/channel and channel/channel interactions
Sophie Aimon, John Manzi, Daniel Schmidt, Jose Antonio Poveda Larrosa, Patricia Bassereau, Gilman E S Toombes   +5 more
doaj   +1 more source