Results 11 to 20 of about 6,246 (170)

Encapsulation of Living Leishmania Promastigotes in Artificial Lipid Vacuoles. [PDF]

open access: yesPLoS ONE, 2015
After phagocytosis by mammalian macrophages, promastigote forms of Leishmania parasites settle inside intracellular parasitophorous vacuoles (PVs) in which they transform into amastigote forms and replicate.
Carlos E S Guedes   +4 more
doaj   +1 more source

Electrochemiluminescence Imaging of Liposome Permeabilization by an Antimicrobial Peptide: Melittin

open access: yesChemical & Biomedical Imaging, 2023
The permeabilization of liposomes by melittin, an antimicrobial peptide (AMP), has been studied by an electrochemiluminescence (ECL) imaging strategy. The methodology consisted first of encapsulating ECL reagents in sealed giant asymmetrical liposomes ...
Fatma Ben Trad   +8 more
doaj   +1 more source

“Force-From-Lipids” Dependence of the MscCG Mechanosensitive Channel Gating on Anionic Membranes

open access: yesMicroorganisms, 2023
Mechanosensory transduction in Corynebacterium glutamicum plays a major role in glutamate efflux for industrial MSG, whose production depends on the activation of MscCG-type mechanosensitive channels.
Yoshitaka Nakayama   +2 more
doaj   +1 more source

Formation of Giant Unilamellar Proteo-Liposomes by Osmotic Shock [PDF]

open access: yesLangmuir, 2015
Giant unilamellar vesicles (GUVs), composed of a phospholipid bilayer, are often used as a model system for cell membranes. However, the study of proteo-membrane interactions in this system is limited as the incorporation of integral and lipid-anchored proteins into GUVs remains challenging.
Motta, Isabelle   +6 more
openaire   +3 more sources

Fluorescence Resonance Energy Transfer to Detect Plasma Membrane Perturbations in Giant Plasma Membrane Vesicles

open access: yesBio-Protocol, 2023
Disruptions and perturbations of the cellular plasma membrane by peptides have garnered significant interest in the elucidation of biological phenomena.
Mathew Sebastiao   +3 more
doaj   +1 more source

Giant liposomes as delivery system for ecophysiological studies in copepods [PDF]

open access: yesJournal of Experimental Biology, 2006
SUMMARYGiant liposomes are proposed as a potential delivery system in marine copepods, the dominant constituent of the zooplankton. Liposomes were prepared in the same size range as the food ingested by copepods (mean diameter of about 7 μm). The encapsulation of a hydrophilic and high molecular mass fluorescent compound, fluorescein isothiocyanate ...
I. Buttino   +7 more
openaire   +5 more sources

Formation of Oligovesicular Vesicles by Micromanipulation

open access: yesMembranes, 2011
Cell-sized lipid bilayer membrane vesicles (giant vesicles, GVs) or semi-vesicles were formed from egg yolk phosphatidylcholine on a platinum electrode under applied electric voltage by electroformation.
Yukihisa Okumura   +2 more
doaj   +1 more source

Single-molecule analysis of the entire perfringolysin O pore formation pathway

open access: yeseLife, 2022
The cholesterol-dependent cytolysin perfringolysin O (PFO) is secreted by Clostridium perfringens as a bacterial virulence factor able to form giant ring-shaped pores that perforate and ultimately lyse mammalian cell membranes. To resolve the kinetics of
Conall McGuinness   +7 more
doaj   +1 more source

Effects of Lipid Composition and Solution Conditions on the Mechanical Properties of Membrane Vesicles

open access: yesMembranes, 2015
The mechanical properties of cell-sized giant unilamellar liposomes were studied by manipulating polystyrene beads encapsulated within the liposomes using double-beam laser tweezers.
Nobuhiko Kato   +5 more
doaj   +1 more source

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