Results 1 to 10 of about 397 (69)

Rapid and Facile Preparation of Giant Vesicles by the Droplet Transfer Method for Artificial Cell Construction

open access: yesFrontiers in Bioengineering and Biotechnology, 2022
Giant vesicles have been widely used for the bottom-up construction of artificial (or synthetic) cells and the physicochemical analysis of lipid membranes.
Yasuhiro Shimane   +4 more
doaj   +1 more source

Curcumin–Induced Stabilization of Protein–Based Nano-Delivery Vehicles Reduces Disruption of Zwitterionic Giant Unilamellar Vesicles

open access: yesMolecules, 2022
Curcumin-loaded native and succinylated pea protein nanoparticles, as well as zwitterionic giant unilamellar vesicles were used in this study as model bioactive compound loaded-nanoparticles and biomembranes, respectively, to assess bio-nano interactions.
Ogadimma D. Okagu   +2 more
doaj   +1 more source

Visualizing Loss of Plasma Membrane Lipid Asymmetry Using Annexin V Staining

open access: yesBio-Protocol, 2023
Loss of plasma membrane lipid asymmetry contributes to many cellular functions and responses, including apoptosis, blood coagulation, and cell fusion.
Julia Baum   +2 more
doaj   +1 more source

Development of Artificial Cell Models Using Microfluidic Technology and Synthetic Biology

open access: yesMicromachines, 2020
Giant lipid vesicles or liposomes are primarily composed of phospholipids and form a lipid bilayer structurally similar to that of the cell membrane. These vesicles, like living cells, are 5–100 μm in diameter and can be easily observed using an optical ...
Koki Kamiya
doaj   +1 more source

Hierarchical unilamellar vesicles of controlled compositional heterogeneity. [PDF]

open access: yesPLoS ONE, 2012
Eukaryotic life contains hierarchical vesicular architectures (i.e. organelles) that are crucial for material production and trafficking, information storage and access, as well as energy production. In order to perform specific tasks, these compartments
Maik Hadorn   +3 more
doaj   +1 more source

Chemical Analysis of Lipid Boundaries after Consecutive Growth and Division of Supported Giant Vesicles

open access: yesiScience, 2020
Summary: The reproduction of the shape of giant vesicles usually results in the increase of their “population” size. This may be achieved on giant vesicles by appropriately supplying “mother” vesicles with membranogenic amphiphiles. The next “generation”
Augustin Lopez   +3 more
doaj   +1 more source

A Fluorescence-based Assay for Measuring Phospholipid Scramblase Activity in Giant Unilamellar Vesicles

open access: yesBio-Protocol, 2022
Transbilayer movement of phospholipids in biological membranes is mediated by a diverse set of lipid transporters. Among them are scramblases that facilitate rapid bi-directional movement of lipids without metabolic energy input.
Patricia Mathiassen   +1 more
doaj   +1 more source

Giant Vesicles Produced with Phosphatidylcholines (PCs) and Phosphatidylethanolamines (PEs) by Water-in-Oil Inverted Emulsions

open access: yesLife, 2021
(1) Background: giant vesicles (GVs) are widely employed as models for studying physicochemical properties of bio-membranes and artificial cell construction due to their similarities to natural cell membranes.
Boying Xu   +3 more
doaj   +1 more source

Cytology of large neurons in the guinea pig dorsal cochlear nucleus contacting the inferior colliculus

open access: yesEuropean Journal of Histochemistry, 2009
Large neurons in the dorsal cochlear nucleus of the guinea pig which project to the inferior colliculus were identified after injections of the neural tracer WGA-HRP.
L Alibardi
doaj   +3 more sources

Formation dynamics of patchy/Janus DNA condensates in monodisperse giant vesicles generated using microfluidics [PDF]

open access: yesRSC Applied Interfaces
Herein, we demonstrate the one-step formation of uniform patchy or Janus-type DNA condensates within monodisperse giant vesicles. This process was achieved by encapsulating DNA building blocks in monodisperse giant vesicles fabricated using a ...
Hiroaki Suzuki   +6 more
doaj   +1 more source

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