Results 41 to 50 of about 51,146 (259)

Surfactant-free production of biomimetic giant unilamellar vesicles using PDMS-based microfluidics

open access: yesCommunications Chemistry, 2021
The production of giant unilamellar vesicles by microfluidics commonly involves additives, which may interfere with the resultant membrane properties. Here pure lipid GUVs are prepared which exclude residual surfactants and other additives.
Naresh Yandrapalli   +3 more
doaj   +1 more source

A Practical Guide to Preparation and Applications of Giant Unilamellar Vesicles Formed via Centrifugation of Water-in-Oil Emulsion Droplets

open access: yesMembranes, 2023
Giant vesicles (GVs), which are closed lipid bilayer membranes with a diameter of more than 1 μm, have attracted attention not only as model cell membranes but also for the construction of artificial cells.
Yiting Zhang, Haruto Obuchi, Taro Toyota
doaj   +1 more source

Osmotic Shrinkage of Giant Egg-Lecithin Vesicles [PDF]

open access: yesBiophysical Journal, 1980
Osmotic shrinkage of giant egg-lecithin vesicles was observed by phase-contrast microscopy. The vesicles remained or became spherical when shrinking. Small and thick-walled vesicles formed visible fingers attached to the sphere. The water permeability of the single bilayer was found to be 41 micrometers/s.
Boroske, E.   +2 more
openaire   +3 more sources

Active Membrane Fluctuations Studied by Micropipet Aspiration [PDF]

open access: yes, 2001
We present a detailed analysis of the micropipet experiments recently reported in J-B. Manneville et al., Phys. Rev. Lett. 82, 4356--4359 (1999), including a derivation of the expected behaviour of the membrane tension as a function of the areal strain ...
A. N. Radionov   +52 more
core   +2 more sources

Oscillatory phase separation in giant lipid vesicles induced by transmembrane osmotic differentials

open access: yeseLife, 2014
Giant lipid vesicles are closed compartments consisting of semi-permeable shells, which isolate femto- to pico-liter quantities of aqueous core from the bulk.
Kamila Oglęcka   +4 more
doaj   +1 more source

Thermal fluctuations of the lipid membrane determine particle uptake into Giant Unilamellar Vesicles

open access: yesNature Communications, 2023
Particulates, bacteria and viruses wrap into cell membranes. Here the authors use optical tweezers, particle tracking and mathematical modelling to show that the uptake process into giant vesicles is influenced by thermal membrane fluctuations.
Yareni A. Ayala   +3 more
doaj   +1 more source

The Effect of Solutes on the Temperature of Miscibility Transitions in Multi-component Membranes

open access: yes, 2017
We address questions posed by experiments which show that most small-chain alcohols reduce the miscibility transition temperature when added to giant plasma membrane vesicles, but increase that temperature when added to giant unilamellar vesicles.
Allender, D. W., Schick, M.
core   +1 more source

A minimal biochemical route towards de novo formation of synthetic phospholipid membranes. [PDF]

open access: yes, 2019
All living cells consist of membrane compartments, which are mainly composed of phospholipids. Phospholipid synthesis is catalyzed by membrane-bound enzymes, which themselves require pre-existing membranes for function.
Bhattacharya, Ahanjit   +3 more
core   +2 more sources

Bottom‐Up Coacervate‐Based Artificial Cells: Integrating Cellular Hallmarks into Complex Life‐Like Systems

open access: yesAngewandte Chemie, EarlyView.
Current interest in artificial cell research underscores its potential to deepen our understanding of life's fundamental processes. This review highlights advances in bottom‐up coacervate‐based artificial cell engineering via combined integration of cellular hallmarks.
Arjan Hazegh Nikroo   +3 more
wiley   +2 more sources

Bending Elasticity Modulus of Giant Vesicles Composed of Aeropyrum Pernix K1 Archaeal Lipid

open access: yesLife, 2015
Thermally induced shape fluctuations were used to study elastic properties of giant vesicles composed of archaeal lipids C25,25-archetidyl (glucosyl) inositol and C25,25-archetidylinositol isolated from lyophilised Aeropyrum pernix K1 cells.
Julia Genova   +4 more
doaj   +1 more source

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