Osmotically balanced, large unilamellar liposomes that enable sustained bupivacaine release for prolonged pain relief in in vivo rat models [PDF]
Scientific Reports, 2021 To efficiently prolong analgesic effects, we developed osmotically balanced, large unilamellar liposomes (~ 6 μm in diameter) in which highly concentrated bupivacaine (up to 30 mg/mL) was encapsulated, and their sustained bupivacaine release was highly ...
Hyebin Yoo +3 more
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Encapsulation of protein/DNA complexes into unilamellar liposomes via annexin-mediated membrane recruitment and sonication [PDF]
Cell Reports: MethodsSummary: This paper reports an effective protocol to encapsulate native protein/DNA complexes into unilamellar vesicles composed of natural lipids without the use of organic solvents, in physiological buffers, and at low protein/DNA concentrations.
Michael Burger +3 more
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Preparation of unilamellar liposomes v1 [PDF]
, 2023 We present here a protocol for preparing liposomes that can be used to monitor binding of proteins to vesicles of various membrane curvatures. We used this to study the association of the PPM1H phosphatase with highly curved membranes due to its N-terminal amphipathic helix.
Ayan Adhikari
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Encapsulation of Hydrophobic Apigenin into Small Unilamellar Liposomes Coated with Chitosan Through Ethanol Injection and Spray Drying. [PDF]
Food Bioproc Tech, 2023 Ang SS, Thoo YY, Siow LF.
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Interaction of unilamellar liposomes with serum lipoproteins and apolipoproteins
Journal of Lipid Research, 1980 The effect of rat whole blood plasma, serum, serum lipoproteins, and apolipoproteins on the stability of unilamellar liposomes prepared with French pressure cell was evaluated by measuring the release of entrapped carboxyfluorescein and by electron ...
L S Guo +4 more
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Preparation of unilamellar fusogenic liposomes using the Sendai virus.
Folia Pharmacologica Japonica, 1998 We describe a method to prepare unilamellar fusogenic liposomes (FL) that can deliver their content directly into tissue cells and cancer cells of living animals as well as into cultured animal cells. The method consists of three important steps: 1) preparation of unilamellar liposomes encapsulating the molecules to be delivered: 2) preparation of the ...
Mahito Nakanishi, Hiroyuki Mizuguchi
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Effect of ultrasound parameters for unilamellar liposome preparation [PDF]
Ultrasonics Sonochemistry, 2009 In this study, it was investigated the effects of ultrasound, namely power input, distance from ultrasound tip to base of reactor and treatment time, in the formation of liposomes. Results indicate a dependence on cavitation events that are a function of power input, and consequently dependent on the position of the probe within the reaction vessel and
Raquel Silva +3 more
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Macroscopic Consequences of the Action of Phospholipase Con Giant Unilamellar Liposomes [PDF]
Biophysical Journal, 2002 Macroscopic consequences of the formation of diacylglycerol by phospholipase C (PC-PLC) in giant 1-stearoyl-2-oleoyl-sn-glycero-3-phosphocholine (SOPC) unilamellar vesicles (GUVs, diameter 10-100 microm) were studied by phase contrast and fluorescence microscopy.
Juha M. Holopainen +3 more
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Ultrasonic evidence for structural relaxation in large unilamellar liposomes [PDF]
The Journal of the Acoustical Society of America, 1983 The ultrasonic absorption of large unilamellar vesicles (average diameter 0.2 μm) was determined in the frequency range 0.5–5 MHz. The liposomes were composed of a 4:1 mixture by weight of dipalmitoyl phosphatidylcholine and dipalmitoyl phosphatidylglycerol.
Philip R. Strom-Jensen +2 more
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Structural–Functional Customization of Nanoscale Liposome-in-Liposome Systems: Precision Engineering Methodology and Artificial-Intelligence-Driven Design Prospects [PDF]
ResearchLiposome-in-liposome systems with multicompartment structures offer advantages in structural complexity and functional programmability, but their application is limited by poor controllability in conventional fabrication.
Liutao Hu, Jianfeng Cai, Chao Lu
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