Encapsulation of protein/DNA complexes into unilamellar liposomes via annexin-mediated membrane recruitment and sonication [PDF]
Summary: This paper reports an effective protocol to encapsulate native protein/DNA complexes into unilamellar vesicles composed of natural lipids without the use of organic solvents, in physiological buffers, and at low protein/DNA concentrations.
Michael Bürger +1 more
exaly +4 more sources
Application of Various Types of Liposomes in Drug Delivery Systems [PDF]
Liposomes, due to their various forms, require further exploration. These structures can deliver both hydrophilic and hydrophobic drugs for cancer, antibacterial, antifungal, immunomodulation, diagnostics, ophtalmica, vaccines, enzymes and genetic ...
Mehran Alavi +2 more
doaj +2 more sources
Interaction of unilamellar liposomes with serum lipoproteins and apolipoproteins
The effect of rat whole blood plasma, serum, serum lipoproteins, and apolipoproteins on the stability of unilamellar liposomes prepared with French pressure cell was evaluated by measuring the release of entrapped carboxyfluorescein and by electron microscopy.
L S Guo +4 more
openaire +4 more sources
Differential Impact of Ergosterol on Yeast Membranes Exposed to Membrane-Targeting Antimicrobial Compounds. [PDF]
Biomimetic membranes (liposomes) were prepared from yeast phospholipids with variable concentrations of ergosterol and exposed to antimicrobial compounds. Dynamic and electrophoretic light scattering measured changes in size and ζ‐potential to study their interaction with the antimicrobial compounds.
Villacres JI, Shaikhet M, Avis TJ.
europepmc +2 more sources
Formation of homogeneous unilamellar liposomes from an interdigitated matrix
Phospholipid-ethanol-aqueous mixtures containing bilayer-forming lipids and 20-50 wt.% of water form viscous gels. Further hydration of these gels results in the formation of liposomes whose morphology depends upon the lipid type. Upon hydration of gels containing mixtures of the lipids 1-palmitoyl-2-oleoyl-phosphatidylcholine (POPC) and 1-palmitoyl-2 ...
Polozova, Alla +7 more
openaire +3 more sources
Large unilamellar liposomes with low uptake into the reticuloendothelial system
Particulate drug carriers, including liposomes, are rapidly removed from blood by cells of the reticuloendothelial system (RES) with resulting adverse effects on this important host defense system. In order to overcome this and other major disadvantages of liposomes, we have altered liposome composition in an effort to achieve prolonged circulation ...
Allen, T.M., Chonn, A.
openaire +3 more sources
Diffusion of hydrogen peroxide across DPPC large unilamellar liposomes
The decomposition of hydrogen peroxide catalyzed by catalase entrapped in the pool of dipalmitoylphosphatidyl choline unilamellar liposomes has been studied. The rate of the process was evaluated by following the production of oxygen as a function of time.
Elsa Abuín +2 more
exaly +5 more sources
Niosomes as a Novel Pharmaceutical Carrier: Structure, Classification and Preparation Methods [PDF]
Niosomes are non-ionic surfactant vesicles obtained by a hydrating mixture of cholesterol and nonionic surfactants. It can be used as a carrier of an amphiphilic and lipophilic drugs.
mona mahmoud
doaj +1 more source
Various methods have been developed to generate phosphoglyceride liposomes. Approaches resulting in homogeneous populations of unilamellar bilayer vesicles are generally preferred to mimic various cell membrane situations, as well as to optimize aqueous ...
Yong Gao +3 more
doaj +1 more source
Ultraviolet A-Induced Peroxidation of Phosphatidylcholine in Unilamellar Liposomes [PDF]
Unilamellar liposomes of 1-palmitoyl-2-linoleoyl-3-sn-phosphatidylcholine (PLPC) were exposed to ultraviolet A (UVA) light at 37 degrees C. The UVA-induced PLPC peroxidation was inhibited by free radical scavengers, while singlet oxygen quenchers showed slight inhibition. When pre-existing hydroperoxides were reduced, UVA irradiation could not initiate
MAZARI, Azzedine +2 more
openaire +2 more sources

