Electroformation of Giant Unilamellar Vesicles from Damp Films in Conditions Involving High Cholesterol Contents, Charged Lipids, and Saline Solutions [PDF]
MembranesGiant unilamellar vesicles (GUVs) are frequently used as membrane models in studies of membrane properties. They are most often produced using the electroformation method.
Ivan Mardešić +2 more
doaj +2 more sources
Instant Membrane Stabilization by Damage‐Triggered Actin Polymerization in Giant Unilamellar Vesicles [PDF]
ChemBioChem, Volume 27, Issue 6, 27 March 2026.Damage‐triggered actin polymerization stabilizes membrane pores in giant unilamellar vesicles (GUVs). Membrane poration drives divalent cation influx, inducing rapid F‐actin assembly at the defect site. The resulting actin plug mechanically arrests pore expansion and preserves vesicle morphology, recapitulating the “plugging” phase of single‐cell ...
Huong Thanh Nguyen +3 more
wiley +2 more sources
Electroformation of Giant Unilamellar Vesicles from Damp Lipid Films with a Focus on Vesicles with High Cholesterol Content [PDF]
MembranesGiant unilamellar vesicles (GUVs) are membrane models used to study membrane properties. Electroformation is one of the methods used to produce GUVs. During electroformation protocol, dry lipid film is formed.
Ivan Mardešić +2 more
doaj +2 more sources
Developing giant plasma membrane vesicles from Leishmania cells to investigate the role of membrane proteins in photodynamic inactivation [PDF]
Photochemistry and Photobiology, Volume 102, Issue 2, Page 480-488, March/April 2026.In this study, we developed giant plasma membrane vesicles (GPMVs) from Leishmania amazonensis promastigote membranes to investigate the role of protein‐embedded membranes in methylene blue (MB) photooxidation. Upon MB photoactivation in model lipid membranes (giant unilamellar vesicles, GUVs), a distinct photooxidation effect was observed, marked by ...
Maressa D. F. de Souza +3 more
wiley +2 more sources
Functional reconstitution of plant plasma membrane H+-ATPase into giant unilamellar vesicles [PDF]
Scientific ReportsMembrane transporters are essential for numerous biological processes by controlling the movement of ions and molecules across cell membranes. However, dissecting their molecular dynamics in complex cellular environments presents significant challenges ...
Huriye D. Uzun +3 more
doaj +2 more sources
The impact of sphingomyelin and cholesterol on ordered lipid domain formation in the bovine milk fat globule membrane using artificial giant unilamellar vesicles as a model [PDF]
JDS CommunicationsGiant unilamellar vesicle (GUV) bilayers were constructed from polar lipids and cholesterol by electroformation as a model system to investigate the formation of ordered lipid domains (OLD) within the milk fat globule membrane (MFGM).
Haotian Zheng +2 more
doaj +2 more sources
Combining Nanoscale Curvature and Polymer Osmotic Pressure for Efficient Giant Vesicle Assembly Under Physiological Conditions [PDF]
Small Methods, Volume 9, Issue 12, December 1, 2025.Polymer‐coated nanocellulose paper (PCP) combines nanoscale curvature and polymer osmotic pressure to promote high‐yield giant vesicle assembly under physiological conditions. PCP‐assisted hydration is scalable, compatible with diverse polymers and biochemical encapsulants, and enables robust GUV formation from small to large substrates across a wide ...
Alexis Cooper +3 more
wiley +2 more sources
A Microfluidic Platform for Actin‐Based Membrane Remodeling Reveals the Stabilizing Role of Branched Actin Networks on Lipid Microdomains [PDF]
Small Science, Volume 5, Issue 10, October 2025.A microfluidic assay to study actin‐driven shape changes of giant unilamellar vesicles (GUVs) is developed. This system enables high‐throughput analysis of membrane remodeling and reveals how actin networks and lipid domains influence each other. It offers a powerful tool to dissect membrane deformation mechanisms in controlled environments. Cell shape
Lixin Huang +9 more
wiley +2 more sources
Control of artificial membrane fusion in physiological ionic solutions beyond the limits of electroformation [PDF]
Nature CommunicationsMembrane fusion, merging two lipid bilayers, is crucial for fabricating artificial membrane structures. Over the past 40 years, in contrast to precise and controllable membrane fusion in-vivo through specific molecules such as SNAREs, controlling the ...
Bong Kyu Kim +7 more
doaj +2 more sources
Radon Mitigation Applications at the Laboratorio Subterráneo de Canfranc (LSC)
Universe, 2022 The Laboratorio Subterráneo de Canfranc (LSC) is the Spanish national hub for low radioactivity techniques and the associated scientific and technological applications.
Javier Pérez-Pérez +18 more
doaj +1 more source