Results 11 to 20 of about 3,400 (188)

Instant Membrane Stabilization by Damage-Triggered Actin Polymerization in Giant Unilamellar Vesicles. [PDF]

Chembiochem
Damage‐triggered actin polymerization stabilizes membrane pores in giant unilamellar vesicles (GUVs). Membrane poration drives divalent cation influx, inducing rapid F‐actin assembly at the defect site. The resulting actin plug mechanically arrests pore expansion and preserves vesicle morphology, recapitulating the “plugging” phase of single‐cell ...
Nguyen HT, Lee SH, Kim CH, Shin K.
europepmc   +2 more sources

Developing giant plasma membrane vesicles from Leishmania cells to investigate the role of membrane proteins in photodynamic inactivation. [PDF]

Photochem Photobiol
In this study, we developed giant plasma membrane vesicles (GPMVs) from Leishmania amazonensis promastigote membranes to investigate the role of protein‐embedded membranes in methylene blue (MB) photooxidation. Upon MB photoactivation in model lipid membranes (giant unilamellar vesicles, GUVs), a distinct photooxidation effect was observed, marked by ...
de Souza MDF, Ciancaglini P, Itri R, Ribeiro MS.   +3 more
europepmc   +2 more sources

Combining Nanoscale Curvature and Polymer Osmotic Pressure for Efficient Giant Vesicle Assembly Under Physiological Conditions. [PDF]

Small Methods
Polymer‐coated nanocellulose paper (PCP) combines nanoscale curvature and polymer osmotic pressure to promote high‐yield giant vesicle assembly under physiological conditions. PCP‐assisted hydration is scalable, compatible with diverse polymers and biochemical encapsulants, and enables robust GUV formation from small to large substrates across a wide ...
Cooper A, Vijayananda V, Pazzi J, Subramaniam AB.   +3 more
europepmc   +2 more sources

A Microfluidic Platform for Actin-Based Membrane Remodeling Reveals the Stabilizing Role of Branched Actin Networks on Lipid Microdomains. [PDF]

Small Sci
A microfluidic assay to study actin‐driven shape changes of giant unilamellar vesicles (GUVs) is developed. This system enables high‐throughput analysis of membrane remodeling and reveals how actin networks and lipid domains influence each other. It offers a powerful tool to dissect membrane deformation mechanisms in controlled environments. Cell shape
Huang L, Lopes Dos Santos R, Labdi S, Lamour G, Maciejak O, Malo M, Manzi J, Lenz M, Fattaccioli J, Campillo C.   +9 more
europepmc   +2 more sources

Endoplasmic reticulum phospholipid scramblase activity revealed after protein reconstitution into giant unilamellar vesicles containing a photostable lipid reporter

Scientific Reports, 2021
Transbilayer movement of phospholipids in biological membranes is mediated by a diverse set of lipid transporters. Among them are scramblases that facilitate a rapid bi-directional movement of lipids without metabolic energy input. Here, we established a
Patricia P. M. Mathiassen, Anant K. Menon, Thomas Günther Pomorski   +2 more
doaj   +1 more source

Effect of an electric field on a floating lipid bilayer: a neutron reflectivity study [PDF]

, 2006
We present here a neutron reflectivity study of the influence of an alternative electric field on a supported phospholipid double bilayer. We report for the first time a reproducible increase of the fluctuation amplitude leading to the complete unbinding
Angelova, Angelova, Atanasov, Burgess, Charitat, Constantin, Cubitt, Daillant, G. Fragneto, Helfrich, Isambert, Lecuyer, Mecke, Penfold, Purrucker, Rosemberg, S. Lecuyer, Sens, T. Charitat, Weaver   +19 more
core   +4 more sources

Frequency-Dependent Electroformation of Giant Unilamellar Vesicles in 3D and 2D Microelectrode Systems

Micromachines, 2017
A giant unilamellar vesicle (GUV), with similar properties to cellular membrane, has been widely studied. Electroformation with its simplicity and accessibility has become the most common method for GUV production.
Qiong Wang, Xiaoling Zhang, Ting Fan, Zhong Yang, Xi Chen, Zhenyu Wang, Jie Xu, Yuanyi Li, Ning Hu, Jun Yang   +9 more
doaj   +1 more source

Electroformation of Giant Vesicles on a Polymer Mesh

Membranes, 2011
Electroformation of cell-sized lipid membrane vesicles (giant vesicles, GVs) from egg yolk phosphatidylcholine under applied electric voltage was examined on a substrate of a polymer mesh placed between two planar indium tin oxide coated glass electrodes.
Yukihisa Okumura, Takuya Sugiyama
doaj   +1 more source

Selective flow-induced vesicle rupture to sort by membrane mechanical properties [PDF]

, 2015
International audienceVesicle and cell rupture caused by large viscous stresses in ultrasonication is central to biomedical and bioprocessing applications.
Brooks, NJ, Garbin, V, Pommella, A, Seddon, JM   +3 more
core   +2 more sources

Comparative Studies of the Electrochemical Behavior of Silver Electrode in Chloride, Bromide and Iodide Aqueous Solutions

International Journal of Electrochemical Science, 2010
Comparative studies of the electrochemical behaviour of Ag in aqueous NaCl, NaBr and NaI solutions of various concentrations ranging from 0.1 M to 1.0 M have been made by means of cyclic voltammetry and chronoamperometry techniques, complemented with SEM
Hamdy H. Hassan, Magdy A.M. Ibrahim, Sayed S. Abd El Rehim, Mohammed A. Amin   +3 more
doaj   +1 more source