Results 141 to 150 of about 6,328,289 (290)

A Leishmania infantum FML-ELISA for the Detection of Symptomatic and Asymptomatic Canine Visceral Leishmaniasis in an Endemic Area of Iran [PDF]

Background: Visceral leishmaniasis (VL) is caused by Leishmania infantum in Mediterranean basin and is an endemic disease in some parts of Iran. Canines are the main reservoirs of VL in most of the endemic areas.
زارعی, ذبیح اله, محمدی قلعه بین, بهنام, محبعلی, مهدی, سرکاری, بهادر, جبیری پور, منصوره, حاتم, غلامرضا, بهلولی, شهاب   +6 more
core  

Detection of potato virus Y and potato leafroll virus in potatoes by two ELISA variants [PDF]

, 1994
Es wurden zwei ELISA-Varianten, der Biotin-Streptavidin-ELISA und der konventionelle „double antibody sandwich" (DAS)-ELISA verglichen im Hinblick auf ihre Eignung, Kartoffelvirus Y (PVY) zuverlässig in Knollen, Keimen und Blättern und ...
Weidemann, H.-L.
core  

Comparison of DAS-ELISA and RT-PCR Methods for the Detection of Prunus Necrotic Ringspot Virus (PNRSV)

, 2005
Two methods (DAS-ELISA and RT-PCR) were compared for the detection of Prunus necrotic ringspot virus (PNRSV) inwoody host Prunus mahleb isolated from Malatya. Total RNA extractions were made from serially diluted fresh leaf tissue, root, barkand one year old green bark tissue using silica-based method.
Usta, Mustafa, Sipahioğlu, Hikmet Murat, Polat, Bülent   +2 more
openaire   +1 more source

Bacteria‐Trained, MnO2‐Armored Dendritic Cells for Immunotherapy

Advanced Materials, EarlyView.
MnO2‐armored, bacteria‐trained dendritic cells undergo tumor‐responsive mechanical and immunological transformation. The nanocoating masks immune ligands and maintains a mechanically stiff, quiescent state during circulation, while the tumor microenvironment removes the armor and restores cellular deformability and immune accessibility.
Zhao Guo, Min Ge, Zesong Ruan, Tingwang Shi, Mahyar Mahmoudi, Soohwan An, Na Kong, Yunfeng Chen, Han Lin, Wei Tao   +9 more
wiley   +1 more source

GaSex Nanoparticles Modulate Neutrophil Function and Mitigate NET‐Driven Osteogenic Impairment in Osteomyelitis Therapy

Advanced Materials, EarlyView.
Neutrophil extracellular traps (NETs) emerge as a critical repair‐limiting factor in osteomyelitis, driving osteoblast mitochondrial dysfunction and excessive PINK1/Parkin‐mediated mitophagy. Gallium–selenium nanoparticles (GaSex) scavenge reactive oxygen species, redirect neutrophils from NETosis toward bactericidal phagocytosis, eliminate methicillin‐
Kai Ye, Xuanzhe Liu, Zifan Pei, Chenyi Jiang, Xiaofen Zhang, Lin Lin, Qihang Fang, Binyu Chen, Longxiang Shen, Liang Cheng, Peng Ding, Xianyou Zheng   +11 more
wiley   +1 more source

A U‐shaped Microfluidic Platform for Culturing Vascularized Colorectal Cancer Spheroids Reveals Tumor–Endothelial Crosstalk and Invasion‐Associated Remodeling

Advanced Materials Technologies, EarlyView.
A closed U‐shaped microfluidic platform symmetrically embeds a single, size‐controlled HT29 colorectal cancer spheroid within a GFP‐HUVEC‐laden fibrin matrix. HT29 spheroids are associated with hyper‐branched endothelial network remodeling, while vascularized co‐culture is accompanied by anisotropic protrusive growth and EMT‐like marker changes in the ...
G. Cretti, M. Ballerini, A. Mantegazza, P. Occhetta, M. Rasponi   +4 more
wiley   +1 more source

Detection of Erwinia chrysanthemi pv dianthicola (Ech) by the DAS-ELISA method in symptomless carnation cuttings

, 1996
This method, using a polyclonal antiserum, the specificity of which was evaluated in this study, can detect up to 5 x 103 cfu.mL-1. Different incubation conditions of carnation stem samples to be tested by DAS-ELISA were studied, in order to amplify the primary inoculum in weakly-infected symptomless samples.
Nassar, A., Narcy, J.P., Lemattre, M.
openaire   +1 more source

Silk Fibroin Nanofiber–Immobilized Horseradish Peroxidase Enables Continuous Preparation of Injectable Hydrogels With Negligible Residual Enzyme for Drug Delivery

Advanced Materials Technologies, EarlyView.
Overcoming the immunogenicity challenges of enzyme‐mediated crosslinking, a flow‐through reactor utilizing SFNF‐HRP enables the continuous production of injectable hydrogels. This continuous platform ensures homogeneous crosslinking while minimizing residual HRP in the resulting hydrogels.
Ayari Miyawaki, Shinji Sakai
wiley   +1 more source

Identificação de Tospovírus em hortaliças no Submédio São Francisco utilizando DAS-ELISA e Dot-ELISA. [PDF]

, 2018
Coletaram-se 216 amostras de folhas com sintomas suspeitos de vira-cabeca, de plantas de tomate (128), cebola (55), pimentao (16) e alface (17), em 22 lotes dos Perimetros Irrigados Senador Nilo Coelho (Petrolina- PE) e Mandacaru, Juazeiro- BA e, Fazenda
POZZER, L., RESENDE, R. de O., LIMA, M. F., MARANHAO, E. A. A., AVILA, A. C. de, FERRAZ, E., CANDEIA, J. A., COSTA, N. D.   +7 more
core  

Self‐Sealing Microneedles Improve Precision and Reduce Reflux in Subretinal Cell Transplantation

Advanced Materials Technologies, EarlyView.
Ultrafine 49G microneedles enable subretinal delivery of induced pluripotent stem cell‐derived retinal pigment epithelial cells through a 50 µm outer diameter tip. Compared with conventional 38G microcannulas, these microneedles reduce cell suspension reflux in a rabbit model, highlighting the potential of microinvasive delivery instruments to improve ...
Jared Ching, Hinako Ichikawa, Aya Motoyoshi, Shin Tanaka, Liam Morrow, Satoshi Shirae, Noriko Sakai, Shohei Kitahata, Michiko Mandai, Kazuaki Kadonosono   +9 more
wiley   +1 more source