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The essential prerequisites for quantitative RT-PCR
Nature Biotechnology, 1999openaire +2 more sources
[Improved RT-PCR: one-step RT-PCR and mRNA selective PCR].
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme, 1999J, Yamamoto, H, Mukai
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RT-PCR negative COVID-19 [PDF]
BMC Infectious Diseases, 2022 Background COVID-19 is a multi-system infection with emerging evidence-based antiviral and anti-inflammatory therapies to improve disease prognosis. However, a subset of patients with COVID-19 signs and symptoms have repeatedly negative RT-PCR tests ...
Heta Parmar +17 more
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Quantitative RT-PCR: Limits and Accuracy
BioTechniques, 1996 In this paper we determine the limits and accuracy of quantitative reverse transcription (RT)-PCR using a modification of the original protocol. The quantification of mRNA with this procedure requires a preliminary estimation of the target molecule (TM ...
F. Souazé +4 more
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Evaluation of the Effects of DNase Treatment on Signal Specificity in RT-PCR and In Situ RT-PCR [PDF]
BioTechniques, 1998 In this study, the effects of DNase treatment on the specificity of reverse transcription (RT)-PCR have been investigated on different samples, containing RNA, DNA, DNA/RNA and DNA/cDNA.
Karin Ivarsson, Birgitta Weijdegård
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