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Quantitative RT-PCR: Limits and Accuracy
BioTechniques, 1996 In this paper we determine the limits and accuracy of quantitative reverse transcription (RT)-PCR using a modification of the original protocol. The quantification of mRNA with this procedure requires a preliminary estimation of the target molecule (TM ...
F. Souazé +4 more
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Comparison of Three RT-PCR Methods [PDF]
BioTechniques, 1998 Various approaches can now be taken for amplification of RNA transcripts using the polymerase chain reaction (PCR). Here, we compare three such methods: (i) uncoupled reverse transcription (RT)-PCR (using separate reactions for cDNA synthesis and PCR ...
Loryn N. Sellner, Gavin R. Turbett
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Evaluation of the Effects of DNase Treatment on Signal Specificity in RT-PCR and In Situ RT-PCR [PDF]
BioTechniques, 1998 In this study, the effects of DNase treatment on the specificity of reverse transcription (RT)-PCR have been investigated on different samples, containing RNA, DNA, DNA/RNA and DNA/cDNA.
Karin Ivarsson, Birgitta Weijdegård
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Quantitative RT-PCR: Pitfalls and Potential
BioTechniques, 1999 Reverse transcription PCR (RT-PCR) represents a sensitive and powerful tool for analyzing RNA. While it has tremendous potential for quantitative applications, a comprehensive knowledge of its technical aspects is required. Successful quantitative RT-PCR
Willard M. Freeman +2 more
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Update Dental College Journal, 2022 Background: In late December 2019, an outbreak of pneumonia with an unknown cause occurs in Wuhan, China. Later, the causative agent was identified as SARS-CoV-2.
Sumaiya khatun +5 more
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BMC Infectious Diseases, 2022 Background COVID-19 is a multi-system infection with emerging evidence-based antiviral and anti-inflammatory therapies to improve disease prognosis. However, a subset of patients with COVID-19 signs and symptoms have repeatedly negative RT-PCR tests ...
Heta Parmar +17 more
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Clinical importance of zinc as monotherapy in modulating
Pakistan Journal of Pharmaceutical Sciences, 2023 Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is globally concerning for overall health. The viral burden is diagnosed by the positive cycle threshold value (Ct-value) of the real-time reverse transcription polymerase chain ...
RT-PCR cycle threshold values +4 more
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A brief history of RT-PCR and our laboratory experience with SARS-CoV-2 analyses using RT-PCR
Jurnal Teknologi Laboratorium, 2022 Polymerase Chain Reaction (PCR) is in vitro replication that allows accelerated amplification of certain sequences in small DNA fragments. A sensitive technique, only traces of DNA are needed for PCR to produce enough copies to be analyzed.
Naci Omer Alayunt
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SARS-CoV-2 RT-PCR positivity rate and predictor factors for RT-PCR results in COVID-19 disease
The Journal of Association of Chest Physicians, 2023 Background/Aim: In December 2019, a novel coronavirus was identified and caused the SARS-CoV-2 (COVID-19) pandemic. The gold standard diagnostic method is the detection of nucleic acid in respiratory tract samples by real-time polymerase chain reaction ...
Melike Badoglu +2 more
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