Results 31 to 40 of about 2,512,760 (207)

Viral Dynamics and Real-Time RT-PCR Ct Values Correlation with Disease Severity in COVID-19

Diagnostics, 2021
Real-time RT-PCR is considered the gold standard confirmatory test for coronavirus disease 2019 (COVID-19). However, many scientists disagree, and it is essential to understand that several factors and variables can cause a false-negative test.
A. Rabaan, R. Tirupathi, A. Sule, J. Aldali, Abbas Al Mutair, S. Alhumaid, Muzaheed, N. Gupta, T. Koritala, Ramesh Adhikari, M. Bilal, Manish Dhawan, R. Tiwari, S. Mitra, T. bin Emran, K. Dhama   +15 more
semanticscholar   +1 more source

Use of Chest CT in Combination with Negative RT-PCR Assay for the 2019 Novel Coronavirus but High Clinical Suspicion

Radiology, 2020
nucleic acid assay was also negative at this time. Six days after admission, the third RT-PCR 2019-nCoV nucleic acid assay was finally found to be positive. When specimen tests are negative, the possibility of a false-negative result should be considered
Pei-Kai Huang, Tian-Zhu Liu, Le-Sheng Huang, Hai-Long Liu, Ming Lei, Wangdong Xu, Xiao-Lu Hu, Jun Chen, Bo Liu   +8 more
semanticscholar   +1 more source

Recent advances and challenges of RT-PCR tests for the diagnosis of COVID-19

Pathology, Research and Practice, 2021
Since the outbreak of the novel severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the control of virus spread has remained challenging given the pitfalls of the current diagnostic tests.
Manoucher Teymouri, S. Mollazadeh, H. Mortazavi, Zari Naderi Ghale-noie, Vahideh Keyvani, Farzaneh Aghababaei, Michael R Hamblin, Ghasem Abbaszadeh-Goudarzi, Hossein Pourghadamyari, S. Hashemian, H. Mirzaei   +10 more
semanticscholar   +1 more source

A novel method for real time quantitative RT-PCR.

Genome Research, 1996
A novel approach to quantitative reverse transcriptase polymerase chain reaction (QC RT-PCR) using real time detection and the 5' nuclease assay has been developed.
Ursula E. M. Gibson, C. Heid, P. M. Williams   +2 more
semanticscholar   +1 more source

False negative of RT‐PCR and prolonged nucleic acid conversion in COVID‐19: Rather than recurrence

Journal of Medical Virology, 2020
A novel coronavirus (COVID-19) pandemic cause by Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) threatens the world. We read with interest the recent report by Li et al.
A. Xiao, Yi Tong, Sheng Zhang
semanticscholar   +1 more source

Massive and rapid COVID-19 testing is feasible by extraction-free SARS-CoV-2 RT-PCR

Nature Communications, 2020
Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is commonly diagnosed by reverse transcription polymerase chain reaction (RT-PCR) to detect viral RNA in patient samples, but RNA extraction ...
Ioanna Smyrlaki, M. Ekman, A. Lentini, Nuno Rufino de Sousa, Natali Papanicolaou, M. Vondráček, Johan Aarum, H. Safari, S. Muradrasoli, A. Rothfuchs, J. Albert, Björn Högberg, Björn Reinius   +12 more
semanticscholar   +1 more source

The screening value of RT-LAMP and RT-PCR in the diagnosis of COVID-19: systematic review and meta-analysis

Journal of Virological Methods, 2021
The purpose of this systematic review is to evaluate the test accuracy of reverse-transcription loop-mediated isothermal amplification (RT-LAMP) and reverse transcription-PCR (RT-PCR) for the diagnosis of coronavirus disease 2019 (COVID-19).
Rui-Yang Pu, Sha Liu, Xiao-Yu Ren, Dian Shi, Y. Ba, Yan-Bei Huo, Wen-Ling Zhang, Lingling Ma, Yan-Yan Liu, Yan Yang, N. Cheng   +10 more
semanticscholar   +1 more source

Comparison of seven commercial RT-PCR diagnostic kits for COVID-19

Journal of Clinical Virology, 2020
The final months of 2019 witnessed the emergence of a novel coronavirus in the human population. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has since spread across the globe and is posing a major burden on society.
P. V. van Kasteren, B. van der Veer, S. van den Brink, L. Wijsman, J. de Jonge, A. van den Brandt, R. Molenkamp, C. Reusken, A. Meijer   +8 more
semanticscholar   +1 more source

Application of standard and molecular methods for the diagnosis of newcastle disease [PDF]

Archives of Biological Sciences, 2012
Four pooled samples of whole poultry carcasses with their internal organs were used to determine the presence of Newcastle disease (ND) virus. Samples were collected from one epizootiological area in the Republic of Serbia during January 2007 ...
Vidanović D., Šekler M., Polaček V., Vasković N., Ašanin Ružica, Milić N., Nišavić J.   +6 more
doaj   +1 more source

Molekularbiologische Diagnostik von Pflanzenkrankkeiten

Julius-Kühn-Archiv, 2014
In der Abteilung für Molekularbiologische Diagnose von Pflanzenkrankheiten der AGES werden molekularbiologische Diagnoseverfahren eingesetzt um gesetzliche Kontroll- und Vollzugsaufgaben zu erfüllen, aber auch um Untersuchungen auf pflanzliche ...
Grausgruber-Gröger, Sabine, Gottsberger, Richard A., Leichtfried, Thomas, Reisenzein, Helga   +3 more
doaj   +1 more source