Results 31 to 40 of about 1,917,084 (305)

Performance of an alternative RT-PCR procedure using residual sample from the Panbio™ Ag COVID-19 test

open access: yesBrazilian Journal of Infectious Diseases, 2021
Introduction: In the current standard of care (SoC) RT-PCR method for COVID-19, the patient's swab was extracted in viral transport media (VTM). For the Panbio™ COVID-19 Ag Rapid Test, the patient swab is flushed out in extraction buffer, of which a ...
Terezinha Marta Pereira Pinto Castineiras   +8 more
doaj   +1 more source

BREAST CANCER TYPING USING RT-PCR ASSAY

open access: yesСибирский онкологический журнал, 2019
Introduction. Adjuvant systemic therapy remains one of the main options for treating breast cancer. Results of standard immunohistochemical studies are not always a criterion for selecting systemic therapy.
V. K. Bozhenko   +6 more
doaj   +1 more source

Optimisation of COVID‐19 diagnostic pathways in acute hospital admissions to prevent nosocomial transmission

open access: yesThe Clinical Respiratory Journal, 2022
Introduction In the management of acute hospital admissions during the COVID‐19 pandemic, safe patient cohorting depends on robust admission diagnostic strategies.
Robert Livingstone   +6 more
doaj   +1 more source

An epithelial GPR35 isoform supports tumor‐associated transcriptional and metabolic phenotypes

open access: yesFEBS Letters, EarlyView.
GPR35 generates two functionally distinct isoforms with previously unresolved roles. GPR35‐short mediates immune‐cell chemotaxis, while GPR35‐long is enriched in colorectal cancer epithelium, where it supports increased metabolism, proliferation, and tumor‐associated transcriptional programs.
Jørgen D. Rønneberg   +14 more
wiley   +1 more source

RT-PCR Protocols - Methods in Molecular Biology

open access: yesEuropean Journal of Histochemistry, 2011
“The first record I have of it, is when I made a computer file which I usually did whenever I had an idea, that would have been on the Monday when I got back, and I called it Chain Reaction.POL, meaning polymerase.
Manuela Monti
doaj   +1 more source

Nutrient/TOR signaling controls adipose mitochondrial transcription factor A (TFAM) to regulate organismal growth in Drosophila

open access: yesFEBS Letters, EarlyView.
Animals must match their growth rate to available nutrients. We show that in Drosophila larvae, the nutrient‐sensing TOR kinase controls growth by regulating levels of TFAM, a key regulator of mitochondrial function, in the adipose tissue. When nutrients are abundant, high TOR activity suppresses TFAM, lowering mitochondrial bioenergetic activity and ...
Shrivani Sriskanthadevan‐Pirahas   +4 more
wiley   +1 more source

RT-LAMP and RT-PCR primer sequences.

open access: yes, 2019
RT-LAMP and RT-PCR primer sequences.
Michele Tchokwansi (6635822)   +13 more
core   +1 more source

Circular RT-PCR Assay Using Arabidopsis Samples

open access: yesBio-Protocol, 2015
Post-transcriptional processing is critical for RNA biogenesis, in which conventional functional RNA transcripts are generated, such as messenger RNAs (mRNAs), transfer RNAs (tRNAs) and ribosomal RNAs (rRNAs) for translation as well as emerging non ...
Runlai Hang   +4 more
doaj   +1 more source

Modeling and analysis of competitive RT-PCR [PDF]

open access: yesNucleic Acids Research, 1998
The present studies demonstrate a theoretical and practical framework for the accurate quantitation of gene expression in RNA extracted from microscopic tissue samples. The approaches are developed around competitive RT-PCR techniques. Assay performance has been examined and validated at both the RT and PCR steps.
A L, Hayward   +5 more
openaire   +2 more sources

The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation

open access: yesFEBS Letters, EarlyView.
Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge   +6 more
wiley   +1 more source

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