Results 51 to 60 of about 2,512,760 (207)
Circular RT-PCR Assay Using Arabidopsis Samples
Bio-Protocol, 2015 Post-transcriptional processing is critical for RNA biogenesis, in which conventional functional RNA transcripts are generated, such as messenger RNAs (mRNAs), transfer RNAs (tRNAs) and ribosomal RNAs (rRNAs) for translation as well as emerging non ...
Runlai Hang +4 more
doaj +1 more source
Respiratory Medicine, 2020 Introduction Chest CT is thought to be sensitive but less specific in diagnosing the 2019 coronavirus disease (COVID-19). The diagnostic value of CT is unclear.
Jianlong He +6 more
semanticscholar +1 more source
Modeling and analysis of competitive RT-PCR [PDF]
Nucleic Acids Research, 1998 The present studies demonstrate a theoretical and practical framework for the accurate quantitation of gene expression in RNA extracted from microscopic tissue samples. The approaches are developed around competitive RT-PCR techniques. Assay performance has been examined and validated at both the RT and PCR steps.
A L, Hayward +5 more
openaire +2 more sources
Evaluation of a saliva molecular point of care for the detection of SARS-CoV-2 in ambulatory care
Scientific Reports, 2021 Rapid identification of SARS-CoV-2-infected individuals is a cornerstone for the control of virus spread. The sensitivity of SARS-CoV-2 RNA detection by RT-PCR is similar in saliva and nasopharyngeal swabs.
Jérôme LeGoff +17 more
doaj +1 more source
Scientific Reports, 2021 Reverse transcriptase polymerase chain reaction (RT-PCR) is a key tool to diagnose Covid-19. Yet it may not be the most efficient test in all patients.
The AP-HP/Universities/Inserm COVID-19 Research Collaboration
doaj +1 more source
Nested-RT-PCR and Multiplex RT-PCR for Diagnosis of Rhinovirus Infection in Clinical Samples [PDF]
, 2014 Human rhinoviruses (HRVs) are positive-stranded RNA viruses belonging to the Enterovirus genus in the family of Picornaviridae. Identification of the specific strain in HRV disease has been difficult because the traditional serological method is insensitive, labor intensive, and cumbersome. With the fast progress in molecular biological technique, more
Yu, Xuelian, Ghildyal, Reena
openaire +2 more sources
International Journal of Infectious Diseases, 2020 In routine clinical practice, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is determined by reverse-transcription PCR (RT-PCR). In the current pandemic, a more rapid and high-throughput method is in growing demand.
Yosuke Hirotsu +14 more
semanticscholar +1 more source
Dynamic profile of RT-PCR findings from 301 COVID-19 patients in Wuhan, China: A descriptive study
Journal of Clinical Virology, 2020 Highlights • This study is currently the largest case series to date on the RT-PCR findings of COVID-19 throughout disease course.• The average contagious period of infected patients was 20 days.• Longer observation period and more than 2 series of ...
A. Xiao +5 more
semanticscholar +1 more source
Rapid quantification of microRNAs in plasma using a fast real-time PCR system
BioTechniques, 2015 The ability to rapidly detect circulating small RNAs, in particular microRNAs (miRNAs), would further increase their already established potential as biomarkers for a range of conditions.
William John Andrews +4 more
doaj +1 more source
Large-scale implementation of pooled RNA extraction and RT-PCR for SARS-CoV-2 detection
Clinical Microbiology and Infection, 2020 Introduction Testing for active SARS-CoV-2 infection is a fundamental tool in the public health measures taken to control the COVID-19 pandemic.
R. Ben-Ami +92 more
semanticscholar +1 more source