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Avian metapneumovirus (AMPV) belongs to Metapneumovirus genus of Paramyxoviridae family. Virus isolation, serology, and detection of genomic RNA are used as diagnostic methods for AMPV.
Helena Lage Ferreira +4 more
doaj
In order to evaluate the health status of Calla and Peruvian lily in Tuscan nurseries, 18 viruses belonging to six families and one unassigned virus were assayed. Tests were carried out on 90 Zantedeschia aethiopica plants and 48 Alstroemeria spp. plants
A. Luvisi +4 more
doaj +1 more source
Should RT‐PCR be considered a gold standard in the diagnosis of COVID‐19?
To face the new Covid-19 pandemic, the need for early and accurate diagnosis of the disease among suspected cases quickly became obvious for effective management, and for better control of the spread of the disease in the population.
M. Dramé +6 more
semanticscholar +1 more source
Quantitative PCR and RT-PCR in virology. [PDF]
In the last few years, molecular hybridization methods have been used extensively in basic and applied virology because of their technical flexibility and high specificity. Using these techniques, the detection of DNA and RNA viruses directly from clinical specimens, the analysis of the specific transcriptional activity of viral genes in vitro and in ...
CLEMENTI M +5 more
openaire +3 more sources
RT-PCR Detection of HIV in Republic of Macedonia
The aim of the study was to detect HIV RNA in seropositive patients using RT-PCR method and thus, to establish PCR methodology in the routine laboratory works.
Golubinka Bosevska +3 more
doaj +1 more source
A case report of COVID-19 with false negative RT-PCR test: necessity of chest CT
The definite diagnosis of corona virus disease 2019 (COVID-19) is based on the viral isolation or positive result of polymerase chain reaction (PCR) from sputum, or nasal swab, or throat swab. However, the sensitivity to detect COVID-19 of real time (RT)-
Hao Feng +3 more
semanticscholar +1 more source
We performed a comprehensive transcript analysis on the early stage of root nodulation in the model legume Lotus japonicus by serial analysis of gene expression (SAGE).
Erika Asamizu +3 more
doaj +1 more source
Estimating false-negative detection rate of SARS-CoV-2 by RT-PCR
Reverse transcription-polymerase chain reaction (RT-PCR) assays are used to test patients and key workers for infection with the causative SARS-CoV-2 virus.
Paul S Wikramaratna +3 more
semanticscholar +1 more source
Incidence of Viral Infection of Main Garlic Growing Areas of Gangwon Province in Korea
This research was conducted to investigate the incidence of viral infection in Samcheok-shi and Jeongseongun, main garlic growing areas of Gangwon province in Korea.
Ji-Eun Shin +2 more
doaj +1 more source
Estimating the false-negative test probability of SARS-CoV-2 by RT-PCR
Background Reverse-transcription PCR (RT-PCR) assays are used to test for infection with the SARS-CoV-2 virus. RT-PCR tests are highly specific and the probability of false positives is low, but false negatives are possible depending on swab type and ...
Paul S Wikramaratna +3 more
semanticscholar +1 more source

