Results 1 to 10 of about 171,737 (259)

A Reverse Transcriptase-Polymerase Chain Reaction Assay for the Detection and Quantitation of Murine Retroviruses

Nature Biotechnology, 1993
Specific hybridization primers for the PCR assay were developed to detect the presence of the ecotropic, xenotropic, and mink cell focus-forming classes of murine leukemia viruses (MuLVs) in samples derived from cultured cells and cell-free supernatants.
J M, Irving, L W, Chang, F J, Castillo
openaire   +2 more sources

Detection and Identification of Flaviviruses by Reverse Transcriptase Polymerase Chain Reaction

1992
We developed a reverse transcriptase/polymerase chain reaction (RT/PCR) for the detection and identification of flavivirus RNA in a single-tube assay. Universal flavivirus RT/PCR amplimers amplify an 832-bp region in the carboxyl portion of NS5 (nt9166-9997) which contains nucleotide sequences conserved among all flaviviruses at the 3′- and 5 ...
Dennis W. Trent, Gwong-Jen Chang
openaire   +1 more source

Quantification of poliovirus in seawater and sewage by competitive reverse transcriptase - polymerase chain reaction

Canadian Journal of Microbiology, 1998
Reverse transcriptase - polymerase chain reaction (RT-PCR) has been used extensively to detect enteric viruses in environmental samples. Advantages of RT-PCR include its high detection sensitivity and rapid turn-around time. However, unlike traditional cell culture, RT-PCR has not provided quantitation and infectivity information.
Y L, Tsai, S L, Parker
openaire   +2 more sources

Detection of Rotaviruses in the Day Care Environment by Reverse Transcriptase Polymerase Chain Reaction

Journal of Infectious Diseases, 1992
Group A rotavirus is an important cause of morbidity among infants and toddlers in day care centers. Transmission by the fecal-oral route is well established, but fomites and environmental surfaces may also play an important role in transmission. A highly sensitive polymerase chain reaction (PCR) assay was used to detect rotavirus RNA in day care ...
J, Wilde, R, Van, L, Pickering, J, Eiden, R, Yolken   +4 more
openaire   +2 more sources

Reverse transcriptase-polymerase chain reaction assay for Acetylcholinesterase mRNA in rat brain

Neurochemical Research, 1995
In order to examine the molecular basis for regional variation in expression of brain acetylcholinesterase (AChE), an assay using reverse transcription and polymerase chain reaction (RT-PCR) was developed to measure steady state levels of AChE mRNA. The amplification method was designed to be specific for templates derived from AChE mRNA and to avoid ...
R, Rao, S, Brimijoin
openaire   +2 more sources

Analysis of Gene Expression by Reverse Transcriptase-Polymerase Chain Reaction

1993
Abstract A molecular genetic approach to development often involves isolating genes controlling cellular developmental processes, analysing their structure and regulation, and gaining clues about their function by examining their expression.
openaire   +1 more source

Processed Pseudogenes Interfere with Reverse Transcriptase-Polymerase Chain Reaction Controls

Analytical Biochemistry, 1996
B, Garbay, E, Boue-Grabot, M, Garret
openaire   +2 more sources

Identification of Bacteria Enduring Endodontic Treatment Procedures by a Combined Reverse Transcriptase–Polymerase Chain Reaction and Reverse-Capture Checkerboard Approach

Journal of Endodontics, 2010
Isabela N RÔÇAS, JOSÉ F Siqueira
exaly  

Application of Real-Time Reverse Transcriptase-Polymerase Chain Reaction in Urological Oncology

Journal of Urology, 2003
Axel Heidenreich, Jan Buer
exaly  

Localization of 12-lipoxygenase mRNA in cultured oligodendrocytes and astrocytes by in situ reverse transcriptase and polymerase chain reaction

Neuroscience Letters, 1995
M Rigaud, J L Beneytout, J M Vallat
exaly