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In-house reverse transcriptase polymerase chain reaction for detection of SARS-CoV-2 with increased sensitivity [PDF]

open access: yesScientific Reports, 2021
As the COVID-19 infection continues to ravage the world, the advent of an efficient as well as the economization of the existing RT-PCR based detection assay essentially can become a blessing in these testing times and significantly help in the ...
Manash Jyoti Kalita   +12 more
doaj   +3 more sources

Diagnosis of Dengue by Using Reverse Transcriptase-Polymerase Chain Reaction [PDF]

open access: yesMemorias do Instituto Oswaldo Cruz, 1997
A rapid identification of dengue viruses from clinical samples by using a nested reverse transcriptase-polymerase chain reaction (RT-PCR) procedure was carried out for diagnostic and epidemiological purposes.
Marize Pereira Miagostovich   +5 more
doaj   +3 more sources

COVID-19 Diagnosed by Real-Time Reverse Transcriptase-Polymerase Chain Reaction in Nasopharyngeal Specimens of Suspected Cases in a Tertiary Care Center: A Descriptive Cross-sectional Study [PDF]

open access: yesJournal of Nepal Medical Association, 2021
Introduction: The emergence of severe acute respiratory syndrome coronavirus-2 pandemic is critically challenging the whole world. The real-time reverse transcriptase-polymerase chain reaction is the most widely used confirmatory test for COVID-19 ...
Narayani Maharjan   +4 more
doaj   +2 more sources

The comparison of chest CT and RT-PCR during the diagnosis of COVID-19 [PDF]

open access: yesĶazaķstannyṇ Klinikalyķ Medicinasy, 2021
Objective: Our aim in this study is to compare the diagnostic accuracy of chest computed tomography and Real-time Reverse Transcriptase Polymerase Chain Reaction in a population with suspected Coronavirus Disease 2019 (COVID-19) presenting at the ...
Avni Uygar Seyhan   +5 more
doaj   +1 more source

Sensitivity and Specificity of High Resolution Computed Tomography Chest in Diagnosing Corona Virus Disease 2019: A Retrospective Correlation with Reverse Transcriptase Polymerase Chain Reaction

open access: yesJournal of Nobel Medical College, 2022
Background: Corona Virus Disease 2019came into existence in December 2019, as a sudden outbreak inthe city of Wuhan, China. Early identification of the patient is vitalto control disease transmission.This study is aimed at estimatingthe sensitivity ...
Robinson Shrestha, Mahesh Gautam
doaj   +1 more source

Experience With Pretravel Testing for SARS-CoV-2 at an Academic Medical Center

open access: yesAcademic Pathology, 2021
International travel has been a significant factor in the coronavirus disease 2019 pandemic. Many countries and airlines have implemented travel restrictions to limit the spread of the causative agent, severe acute respiratory syndrome coronavirus-2.
Katherine L. Imborek MD   +5 more
doaj   +1 more source

Accuracy of Rapid Antigen Detection Test Device (COVID-19 Ag Rapid Test Device) in Diagnosis of Acute COVID-19 Infections

open access: yesPakistan Armed Forces Medical Journal, 2022
Objective: To evaluate the accuracy of the COVID-19 Ag Rapid test device for detection of SARS-CoV-2 with Reverse Transcriptase Polymerase Chain Reaction as the gold standard. Study Design: Cross-sectional study.
Noman Shakoor   +5 more
doaj   +1 more source

Direct in situ reverse transcriptase-polymerase chain reaction [PDF]

open access: yesAmerican Journal of Physiology-Cell Physiology, 2001
In situ hybridization has been used for localization of specific nucleic acid sequences at the cellular level despite providing relatively low-detection sensitivity. In situ reverse transcriptase-polymerase chain reactions (RT-PCR) enhance sensitivity and thus enable localization of low-abundance mRNA in a cell.
R, Kher, R, Bacallao
openaire   +2 more sources

Enteroviral pharyngitis diagnosed by reverse transcriptase-polymerase chain reaction [PDF]

open access: yesInternational Journal of Pediatric Otorhinolaryngology, 1996
The role of enteroviruses in childhood pharyngitis was investigated using enteroviral specific reverse transcriptase-polymerase chain reaction (RT-PCR). Viral/bacterial throat swabs were taken from 50 children with acute pharyngitis and 26 controls.
M, Sharland   +4 more
openaire   +3 more sources

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