Diagnosis of Dengue by Using Reverse Transcriptase-Polymerase Chain Reaction [PDF]
Memorias do Instituto Oswaldo Cruz, 1997 A rapid identification of dengue viruses from clinical samples by using a nested reverse transcriptase-polymerase chain reaction (RT-PCR) procedure was carried out for diagnostic and epidemiological purposes.
Marize Pereira Miagostovich +5 more
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In-house reverse transcriptase polymerase chain reaction for detection of SARS-CoV-2 with increased sensitivity [PDF]
Scientific Reports, 2021 As the COVID-19 infection continues to ravage the world, the advent of an efficient as well as the economization of the existing RT-PCR based detection assay essentially can become a blessing in these testing times and significantly help in the ...
Manash Jyoti Kalita +12 more
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COVID-19 Diagnosed by Real-Time Reverse Transcriptase-Polymerase Chain Reaction in Nasopharyngeal Specimens of Suspected Cases in a Tertiary Care Center: A Descriptive Cross-sectional Study [PDF]
Journal of Nepal Medical Association, 2021 Introduction: The emergence of severe acute respiratory syndrome coronavirus-2 pandemic is critically challenging the whole world. The real-time reverse transcriptase-polymerase chain reaction is the most widely used confirmatory test for COVID-19 ...
Narayani Maharjan +4 more
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Journal of Nobel Medical College, 2022 Background: Corona Virus Disease 2019came into existence in December 2019, as a sudden outbreak inthe city of Wuhan, China. Early identification of the patient is vitalto control disease transmission.This study is aimed at estimatingthe sensitivity ...
Robinson Shrestha, Mahesh Gautam
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Molecularly imprinted nanoparticles for inhibiting ribonuclease in reverse transcriptase polymerase chain reaction [PDF]
The Analyst, 2018 We showed that molecularly imprinted nanoparticles (nanoMIPs) could efficiently inhibit the activities of the RNase in RT-PCR reactions, demonstrating that the tailor-made nanomaterials are very promising for use in routine biological assays.
Xiaotong Feng +4 more
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São Paulo Medical JournalCONTEXT: Cutaneous melanoma presents significant morbidity and mortality. Nowadays, about 90% of them are diagnosed by clinical examination and most are localized melanomas.
Allisson Monteiro da Silva +3 more
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A real-time reverse transcriptase polymerase chain reaction for detection and quantification of Vesiculovirus [PDF]
Memorias do Instituto Oswaldo Cruz, 2016 Vesiculoviruses (VSV) are zoonotic viruses that cause vesicular stomatitis disease in cattle, horses and pigs, as well as sporadic human cases of acute febrile illness. Therefore, diagnosis of VSV infections by reliable laboratory techniques is important
Aline Lavado Tolardo +14 more
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Methods and ProtocolsReverse transcription polymerase chain reaction (RT-PCR) is an important tool for the detection of target RNA molecules and the assay of RNA pathogens.
Evgeniya V. Smirnova +5 more
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The comparison of chest CT and RT-PCR during the diagnosis of COVID-19 [PDF]
Ķazaķstannyṇ Klinikalyķ Medicinasy, 2021 Objective: Our aim in this study is to compare the diagnostic accuracy of chest computed tomography and Real-time Reverse Transcriptase Polymerase Chain Reaction in a population with suspected Coronavirus Disease 2019 (COVID-19) presenting at the ...
Avni Uygar Seyhan +5 more
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A Recent Non cytopathogenic bovine viral diarrhea virus detected by immunofluorescence and reverse transcriptase polymerase chain reaction [PDF]
Benha Veterinary Medical Journal, 2021 In the present study, a non-cytopathogenic bovine viral diarrhea virus (BVDV) field strains from bovine semen samples were detected by immunofluorescence (IF) and reverse transcriptase polymerase chain reaction (RT-PCR).
Ehab ElNahas +3 more
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