FEBS Open Bio, EarlyView.Glioblastoma cells express calcitonin receptor variants (CT receptor isoforms) that may help them survive stress. Using qPCR, transcript‐specific long‐read nanopore sequencing, immunofluorescence co‐localisation and comparative sequence analysis, this study identifies a novel alternatively spliced CALCR transcript that encodes the CTb receptor isoform ...
Pragya Gupta +7 more
wiley +1 more source
Reverse transcriptase-polymerase chain reaction (RT-PCR) primers and conditions.
, 2013 Reverse transcriptase-polymerase chain reaction (RT-PCR) primers and conditions.
Sang-Im Lee (403297) +4 more
core +1 more source
Efficacy of the Examination of Saliva Sample by Reverse Transcriptase-Polymerase Chain Reaction in Detection of SARS-CoV-2 in Al-Fallujah City, Iraq [PDF]
, 2022 Saliva samples could be used as a non-invasive method to diagnose COVID-19. We aimed to assess the results of the reverse transcriptase-polymerase chain reaction (RT-PCR) of saliva specimens in the detection of COVID-19.
Raid M. Al-Ani +2 more
core +1 more source
BCG vaccination potentiates oxidative phosphorylation in neonatal myeloid‐derived suppressor cells
FEBS Open Bio, EarlyView.BCG vaccination enhances oxidative phosphorylation in neonatal MDSCs, impairing their immunosuppressive function. It upregulates electron transport chain genes and mitochondrial activity, increasing ATP and oxygen consumption. Pharmacological OXPHOS inhibition partially restores suppressive capacity, confirming causality.
Yingying Chen, Hui Li
wiley +1 more source
Detection of H275Y Mutation of A(H1N1)pdmO9 Viruses Using a Real-time RT-PCR Assay in Mongolia
Central Asian Journal of Medical Sciences, 2016 Objectives: Oseltamivir is recommended as a first-line drug for the prophylaxis and treatment of influenza A(H1N1)pdmO9 infection worldwide. However, oseltamivir-resistant influenza A(H1N1)pdmO9 viruses have been identified and are mostly associated with
Naranzul Tsedenbal +3 more
doaj +1 more source
Semiquantification of circulating hepatocellular carcinoma cells by reverse transcriptase polymerase chain reaction [PDF]
British Journal of Cancer, 1997 Hepatocellular carcinoma (HCC) is one of the most common and rapidly fatal malignancies worldwide. Treatment options are severely limited by the frequent presence of metastases. If hepatocyte-specific mRNAs are detected in the circulation, it is possible to infer the presence of circulating, presumably malignant, liver cells. If these can be quantified,
I H, Wong +5 more
openaire +2 more sources
Primer sets for reverse transcriptase-polymerase chain reaction analysis.
, 2013 Primer sets for reverse transcriptase-polymerase chain reaction analysis.
Wen-Qi Lai (350121) +7 more
core +1 more source
Chronobiology of Cancer: How Aging Fuels Oncogenesis at the Molecular Level
Aging and Cancer, EarlyView.This graphical abstract illustrates the key biological pathways linking aging with cancer development and progression. In the upper left, cumulative exposure to ultraviolet radiation, toxins, and reactive oxygen species (ROS) causes DNA damage and genomic instability, whereas age‐related decline in repair mechanisms, such as ATM/ATR, BER, and NER ...
Anu Singh, Aroonima Misra, Sufian Zaheer
wiley +1 more source
Primers used for real-time reverse transcriptase polymerase chain reaction.
, 2013 Primers used for real-time reverse transcriptase polymerase chain reaction.
Livio Giuliani (401073) +8 more
core +1 more source
Advanced Science, EarlyView.Glaucoma, a major cause of blindness, involves retinal ganglion cell (RGC) degeneration. This study shows growth hormone‐releasing hormone receptor (GHRHR) deficiency preserves RGC survival and restores vision, unlike activation which only aids survival.
Yan Tong +24 more
wiley +1 more source