Results 31 to 40 of about 171,737 (259)

Enteroviral pharyngitis diagnosed by reverse transcriptase-polymerase chain reaction. [PDF]

open access: yesArchives of Disease in Childhood, 1996
The role of enteroviruses in childhood pharyngitis was investigated using enteroviral specific reverse transcriptase-polymerase chain reaction (RT-PCR). Viral/bacterial throat swabs were taken from 50 children with acute pharyngitis and 26 controls.
M, Sharland   +4 more
openaire   +3 more sources

A semi-quantitative RT-PCR method to measure the in vivo effect of dietary conjugated linoleic acid on porcine muscle PPAR gene expression

open access: yesBiological Procedures Online, 2003
Conjugated linoleic acid (CLA) can activate (in vitro) the nuclear transcription factors known as the peroxisome proliferators activated receptors (PPAR).
Meadus W.J.
doaj   +1 more source

REVERSE TRANSCRIPTASE-POLYMERASE CHAIN REACTION ASSAYS FOR PROSTATE CANCER

open access: yesUrologic Clinics of North America, 1997
RT-PCR, if targeted against a prostate-specific marker, can be a highly specific and sensitive assay to detect the presence of occult prostate cancer cells at sites far distant from the primary tumor. The low false-positive rate (0.8%) observed when combining most published studies and the high rate of detection of prostate cells in metastatic patients
C A, Olsson   +3 more
openaire   +2 more sources

Molecularly imprinted nanoparticles for inhibiting ribonuclease in reverse transcriptase polymerase chain reaction [PDF]

open access: yesThe Analyst, 2018
We showed that molecularly imprinted nanoparticles (nanoMIPs) could efficiently inhibit the activities of the RNase in RT-PCR reactions, demonstrating that the tailor-made nanomaterials are very promising for use in routine biological assays.
Xiaotong Feng   +4 more
openaire   +6 more sources

The polymerase chain reaction-based SARS-CoV-2 detection

open access: yesUniversidad Médica Pinareña, 2020
Introduction: the reverse transcriptase polymerase chain reaction is a highly precise technique in the detection and amplification of genetic material, and therefore in the detection of viruses.
José Francisco Cancino-Mesa   +2 more
doaj   +2 more sources

Capacity of rTth polymerase to detect RNA in the presence of various inhibitors. [PDF]

open access: yesPLoS ONE, 2018
The full potential of the real-time reverse transcription polymerase chain reaction (RT-PCR) as a rapid and accurate diagnostic method is limited by DNA polymerase inhibitors as well as reverse transcriptase inhibitors which are ubiquitous in clinical ...
Dongyang Cai   +4 more
doaj   +1 more source

SARS-CoV-2 RNA detection in cerebrospinal fluid: Presentation of two cases and review of literature

open access: yesBrain, Behavior, & Immunity - Health, 2021
Neurological manifestations of SARS-CoV-2 infection are multiple and heterogeneous. However, confirmation of nervous system impairment by viral RNA detection in cerebrospinal fluid (CSF) is uncommon.
María Belén Luis   +3 more
doaj   +1 more source

Fetal Cytokine Expression In UteroDetected by Reverse Transcriptase Polymerase Chain Reaction [PDF]

open access: yesPediatric Research, 1995
Cytokine expression at the maternal-fetal interface is well documented. Some authors have postulated the existence of a bidirectional cytokine signaling mechanism that is critical to the maintenance of normal pregnancy. The role of the fetus (versus fetally derived placental tissues) in this process is unknown.
J N, Jarvis   +4 more
openaire   +2 more sources

Comparative analysis and identification of spike mutations in Iranian COVID-19 samples from the first three waves of disease

open access: yesAdvanced Biomedical Research, 2023
Background: The spike surface glycoprotein of SARS-CoV-2 is the essential protein in virus attachment to the target cell and cell entrance. As this protein contains immunodominant epitopes and is the main target for immune recognition, it is the critical
Parisa Shoaei   +6 more
doaj   +1 more source

Relevance of micrometastases detected by reverse transcriptase-polymerase chain reaction for melanoma recurrence: systematic review and meta-analysis

open access: yesSão Paulo Medical Journal
CONTEXT: Cutaneous melanoma presents significant morbidity and mortality. Nowadays, about 90% of them are diagnosed by clinical examination and most are localized melanomas.
Allisson Monteiro da Silva   +3 more
doaj   +1 more source

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