Results 31 to 40 of about 3,977,864 (285)

Practical Considerations for Implementation of SARS-CoV-2 Serological Testing in the Clinical Laboratory: Experience at an Academic Medical Center

Academic Pathology, 2021
Molecular techniques, especially reverse transcriptase polymerase chain reaction (RT-PCR), have been the gold standard for the diagnosis of acute severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection.
Robert M. Humble MD, Anna E. Merrill PhD, Bradley A. Ford MD, PhD, Daniel J. Diekema MD, Matthew D. Krasowski MD, PhD   +4 more
doaj   +1 more source

Phylogenetic and molecular characteristics of Eurasian H9 avian influenza viruses and their detection by two different H9-specific RealTime reverse transcriptase polymerase chain reaction tests [PDF]

, 2013
Avian influenza viruses (AIVs) of the H9 haemagglutinin subtype are endemic in many Asian and Middle-East countries, causing mortality and morbidity in poultry.
Krill, D., Govil, J., Mahmood, Sahar, Arnold, Mark E., Shell, Wendy M., Manvell, Ruth J., Brown, Ian H., Slomka, Marek J., Hanna, Amanda, Banks, Jill   +9 more
core   +1 more source

MOLECULAR DETECTION OF ASTROVIRUS USING REVERSE TRANSCRIPTASE QUANTITATIVE POLYMERASE CHAIN REACTION TECHNIQUE

The Iraqi Journal of Agricultural science, 2020
This study was aimed to highlight the importance of the melt curve-quantitative reverse transcriptase PCR (RT-qPCR) analysis in the detection of astrovirus (AstV) from both negative and positive rotavirus and enterovirus (EVs) samples, and the ...
Fadhil & et al.
doaj   +1 more source

Correlation between CSF biomarkers and COVID-19 meningoencephalitis: A case series

Respiratory Medicine Case Reports, 2021
Background: Recent studies have focused on the incidence rate and pattern of meningoencephalitis in the coronavirus disease 2019 (COVID-19). Aim: This study aims to shed more light on the CSF pattern and clinical characteristics of meningoencephalitis ...
Minoosh Moghimi, Amirhossein Moghtader, Manijeh Jozpanahi, Kasra Khodadadi, Mohammad Jafarzade, Zahra Abbaspour   +5 more
doaj   +1 more source

What are we missing? Three cases of severe COVID-19 pneumonia with negative testing

SAGE Open Medical Case Reports, 2020
The COVID-19 pandemic has drastically affected health care systems globally. Reverse transcriptase-polymerase chain reaction is currently the preferred method of detecting COVID-19; however, sensitivity of this test remains questionable.
Aisha Siddiqui, Sukhdev Singh, Zohaib Khan, Allison Foster, Muhammed Atere, Jay M Nfonoyim   +5 more
doaj   +1 more source

Reverse-Transcriptase Polymerase Chain Reaction to Detect Extracellular mRNAs [PDF]

, 2011
The presence of extracellular nucleic acids has been reported in serum/plasma from cancer and diabetes patients that may help in disease diagnosis. Taking insulin-producing cells as examples here, RT-PCR was used to investigate a correlation between the presence and amounts of extracellular mRNA(s) and cell mass and/or function. RT-PCR was performed on
Sweta, Rani, Lorraine, O'Driscoll
openaire   +4 more sources

RIPK3 dampens mitochondrial bioenergetics and lipid droplet dynamics in metabolic liver disease

Hepatology, EarlyView., 2022
RIPK3 dampens mitochondrial bioenergetics and lipid droplet dynamics in metabolic liver disease. Abstract Background and Aims Receptor‐interacting protein kinase 3 (RIPK3) mediates NAFLD progression, but its metabolic function is unclear. Here, we aimed to investigate the role of RIPK3 in modulating mitochondria function, coupled with lipid droplet (LD)
Marta B. Afonso, Tawhidul Islam, Julie Magusto, Ricardo Amorim, Véronique Lenoir, Rui F. Simões, José Teixeira, Liana C. Silva, Dominique Wendum, Isabelle Jéru, Corinne Vigouroux, Rui E. Castro, Paulo J. Oliveira, Carina Prip‐Buus, Vlad Ratziu, Jérémie Gautheron, Cecília M. P. Rodrigues   +16 more
wiley   +1 more source

A semi-quantitative RT-PCR method to measure the in vivo effect of dietary conjugated linoleic acid on porcine muscle PPAR gene expression

Biological Procedures Online, 2003
Conjugated linoleic acid (CLA) can activate (in vitro) the nuclear transcription factors known as the peroxisome proliferators activated receptors (PPAR).
Meadus W.J.
doaj   +1 more source

Expanding molecular diagnostic capacity for COVID-19 in Ethiopia: operational implications, challenges and lessons learnt

The Pan African Medical Journal, 2021
Efforts towards slowing down coronavirus (COVID-19) transmission and reducing mortality have focused on timely case detection, isolation and treatment.
Adisu Kebede, Betty Lanyero, Berhane Beyene, Mayur Lalji Mandalia, Daniel Melese, Feven Girmachew, Adamu Mekonnen, Gonfa Ayana, Nebiyou Yemanebirhan, Getnet Hailu, Habtamu Asrat, Negash Nurahmed, Andargachew Gashu, Kirubel Eshetu, Zewdu Assefa, Aschalew Abayneh, Emmanuel Musa, Ebba Abate   +17 more
doaj   +1 more source

The polymerase chain reaction-based SARS-CoV-2 detection

Universidad Médica Pinareña, 2020
Introduction: the reverse transcriptase polymerase chain reaction is a highly precise technique in the detection and amplification of genetic material, and therefore in the detection of viruses.
José Francisco Cancino-Mesa, Adrián Alejandro Vitón-Castillo, Jorge Casí-Torres   +2 more
doaj   +2 more sources