Diagnosis of Dengue by Using Reverse Transcriptase-Polymerase Chain Reaction [PDF]
Memorias do Instituto Oswaldo Cruz, 1997 A rapid identification of dengue viruses from clinical samples by using a nested reverse transcriptase-polymerase chain reaction (RT-PCR) procedure was carried out for diagnostic and epidemiological purposes.
Marize Pereira Miagostovich +5 more
doaj +7 more sources
COVID-19 Diagnosed by Real-Time Reverse Transcriptase-Polymerase Chain Reaction in Nasopharyngeal Specimens of Suspected Cases in a Tertiary Care Center: A Descriptive Cross-sectional Study [PDF]
Journal of Nepal Medical Association, 2021 Introduction: The emergence of severe acute respiratory syndrome coronavirus-2 pandemic is critically challenging the whole world. The real-time reverse transcriptase-polymerase chain reaction is the most widely used confirmatory test for COVID-19 ...
Narayani Maharjan +4 more
doaj +3 more sources
In-house reverse transcriptase polymerase chain reaction for detection of SARS-CoV-2 with increased sensitivity [PDF]
Scientific Reports, 2021 As the COVID-19 infection continues to ravage the world, the advent of an efficient as well as the economization of the existing RT-PCR based detection assay essentially can become a blessing in these testing times and significantly help in the ...
Manash Jyoti Kalita +12 more
doaj +3 more sources
Journal of Nobel Medical College, 2022 Background: Corona Virus Disease 2019came into existence in December 2019, as a sudden outbreak inthe city of Wuhan, China. Early identification of the patient is vitalto control disease transmission.This study is aimed at estimatingthe sensitivity ...
Robinson Shrestha, Mahesh Gautam
doaj +2 more sources
Relevance of micrometastases detected by reverse transcriptase-polymerase chain reaction for melanoma recurrence: systematic review and meta-analysis [PDF]
São Paulo Medical Journal, 2003 CONTEXT: Cutaneous melanoma presents significant morbidity and mortality. Nowadays, about 90% of them are diagnosed by clinical examination and most are localized melanomas.
Allisson Monteiro da Silva +3 more
doaj +3 more sources
Molecular and Cellular Probes, 1998 A novel method was developed for the detection of thermophilic enteropathogenic campylobacters based on the detection of mRNA using the reverse transcriptase polymerase chain reaction (RT-PCR). The RNA extraction method, DNase treatment and RT-PCR assay were shown to be specific for mRNA.
F J Bolton, A J Fox, D R Wareing
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A real-time reverse transcriptase polymerase chain reaction for detection and quantification of Vesiculovirus [PDF]
Memorias do Instituto Oswaldo Cruz, 2016 Vesiculoviruses (VSV) are zoonotic viruses that cause vesicular stomatitis disease in cattle, horses and pigs, as well as sporadic human cases of acute febrile illness. Therefore, diagnosis of VSV infections by reliable laboratory techniques is important
Aline Lavado Tolardo +14 more
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Quantitative Reverse Transcriptase Polymerase Chain Reaction
, 2007 Since the first documentation of real-time polymerase chain reaction (PCR),(1) it has been used for an increasing and diverse number of applications, including mRNA expression studies, DNA copy number measurements in genomic or viral DNAs,(2–7) allelic discrimination assays,(8,9) expression analysis of specific splice variants of genes(10–13) and gene ...
Gommersall, Lyndon M. +3 more
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Molecularly imprinted nanoparticles for inhibiting ribonuclease in reverse transcriptase polymerase chain reaction [PDF]
The Analyst, 2018 We showed that molecularly imprinted nanoparticles (nanoMIPs) could efficiently inhibit the activities of the RNase in RT-PCR reactions, demonstrating that the tailor-made nanomaterials are very promising for use in routine biological assays.
Xiaotong Feng +4 more
openaire +8 more sources
When to test for COVID-19 using real-time reverse transcriptase polymerase chain reaction: a systematic review [PDF]
International Journal of Infectious Diseases, 2022 Jaderson Dacosta +2 more
exaly +2 more sources