Results 11 to 20 of about 171,737 (259)

In-house reverse transcriptase polymerase chain reaction for detection of SARS-CoV-2 with increased sensitivity [PDF]

Scientific Reports, 2021
As the COVID-19 infection continues to ravage the world, the advent of an efficient as well as the economization of the existing RT-PCR based detection assay essentially can become a blessing in these testing times and significantly help in the ...
Manash Jyoti Kalita, Kalpajit Dutta, Gautam Hazarika, Ridip Dutta, Simanta Kalita, Partha Pratim Das, Manash P. Sarma, Sofia Banu, Md. Ghaznavi Idris, Anjan Jyoti Talukdar, Sangitanjan Dutta, Ajanta Sharma, Subhash Medhi   +12 more
doaj   +3 more sources

Diagnosis of Dengue by Using Reverse Transcriptase-Polymerase Chain Reaction [PDF]

Memorias do Instituto Oswaldo Cruz, 1997
A rapid identification of dengue viruses from clinical samples by using a nested reverse transcriptase-polymerase chain reaction (RT-PCR) procedure was carried out for diagnostic and epidemiological purposes.
Marize Pereira Miagostovich, Flávia Barreto dos Santos, Eliane Saraiva M de Araújo, Juarez Dias, Hermann G Schatzmayr, Rita Maria Ribeiro Nogueira   +5 more
doaj   +3 more sources

COVID-19 Diagnosed by Real-Time Reverse Transcriptase-Polymerase Chain Reaction in Nasopharyngeal Specimens of Suspected Cases in a Tertiary Care Center: A Descriptive Cross-sectional Study [PDF]

Journal of Nepal Medical Association, 2021
Introduction: The emergence of severe acute respiratory syndrome coronavirus-2 pandemic is critically challenging the whole world. The real-time reverse transcriptase-polymerase chain reaction is the most widely used confirmatory test for COVID-19 ...
Narayani Maharjan, Niresh Thapa, Bibek Pun Magar, Muna Maharjan, Jiancheng Tu   +4 more
doaj   +2 more sources

A reverse transcriptase polymerase chain reaction assay for the detection of thermophilicCampylobacterspp.

Molecular and Cellular Probes, 1998
A novel method was developed for the detection of thermophilic enteropathogenic campylobacters based on the detection of mRNA using the reverse transcriptase polymerase chain reaction (RT-PCR). The RNA extraction method, DNase treatment and RT-PCR assay were shown to be specific for mRNA.
F J Bolton, A J Fox, D R A Wareing
exaly   +3 more sources

A real-time reverse transcriptase polymerase chain reaction for detection and quantification of Vesiculovirus [PDF]

Memorias do Instituto Oswaldo Cruz, 2016
Vesiculoviruses (VSV) are zoonotic viruses that cause vesicular stomatitis disease in cattle, horses and pigs, as well as sporadic human cases of acute febrile illness. Therefore, diagnosis of VSV infections by reliable laboratory techniques is important
Aline Lavado Tolardo, William Marciel de Souza, Marilia Farignoli Romeiro, Luiz Carlos Vieira, Luciano Kleber de Souza Luna, Dyana Alves Henriques, Jansen de Araujo, Carlos Eduardo Hassegawa Siqueira, Tatiana Elias Colombo, Victor Hugo Aquino, Benedito Antonio Lopes da Fonseca, Roberta Vieira de Morais Bronzoni, Maurício Lacerda Nogueira, Edison Luiz Durigon, Luiz Tadeu Moraes Figueiredo   +14 more
doaj   +3 more sources

The comparison of chest CT and RT-PCR during the diagnosis of COVID-19 [PDF]

Ķazaķstannyṇ Klinikalyķ Medicinasy, 2021
Objective: Our aim in this study is to compare the diagnostic accuracy of chest computed tomography and Real-time Reverse Transcriptase Polymerase Chain Reaction in a population with suspected Coronavirus Disease 2019 (COVID-19) presenting at the ...
Avni Uygar Seyhan, Rohat Ak, Erdal Yılmaz, Fatih Doğanay, Ömer Aydıner, Serap Demir Tekol   +5 more
doaj   +1 more source

A Recent Non cytopathogenic bovine viral diarrhea virus detected by immunofluorescence and reverse transcriptase polymerase chain reaction [PDF]

Benha Veterinary Medical Journal, 2021
In the present study, a non-cytopathogenic bovine viral diarrhea virus (BVDV) field strains from bovine semen samples were detected by immunofluorescence (IF) and reverse transcriptase polymerase chain reaction (RT-PCR).
Ehab ElNahas, zeinab aboezz, Ayman El-Habbaa, Samia Abdelkareem   +3 more
doaj   +1 more source

Sensitivity and Specificity of High Resolution Computed Tomography Chest in Diagnosing Corona Virus Disease 2019: A Retrospective Correlation with Reverse Transcriptase Polymerase Chain Reaction

Journal of Nobel Medical College, 2022
Background: Corona Virus Disease 2019came into existence in December 2019, as a sudden outbreak inthe city of Wuhan, China. Early identification of the patient is vitalto control disease transmission.This study is aimed at estimatingthe sensitivity ...
Robinson Shrestha, Mahesh Gautam
doaj   +1 more source

Experience With Pretravel Testing for SARS-CoV-2 at an Academic Medical Center

Academic Pathology, 2021
International travel has been a significant factor in the coronavirus disease 2019 pandemic. Many countries and airlines have implemented travel restrictions to limit the spread of the causative agent, severe acute respiratory syndrome coronavirus-2.
Katherine L. Imborek MD, Matthew D. Krasowski MD, PhD, Paul Natvig MD, Anna E. Merrill PhD, Daniel J. Diekema MD, Bradley A. Ford MD, PhD   +5 more
doaj   +1 more source

Direct in situ reverse transcriptase-polymerase chain reaction [PDF]

American Journal of Physiology-Cell Physiology, 2001
In situ hybridization has been used for localization of specific nucleic acid sequences at the cellular level despite providing relatively low-detection sensitivity. In situ reverse transcriptase-polymerase chain reactions (RT-PCR) enhance sensitivity and thus enable localization of low-abundance mRNA in a cell.
R, Kher, R, Bacallao
openaire   +2 more sources