Results 21 to 30 of about 269,716 (296)

What are we missing? Three cases of severe COVID-19 pneumonia with negative testing

open access: yesSAGE Open Medical Case Reports, 2020
The COVID-19 pandemic has drastically affected health care systems globally. Reverse transcriptase-polymerase chain reaction is currently the preferred method of detecting COVID-19; however, sensitivity of this test remains questionable.
Aisha Siddiqui   +5 more
doaj   +1 more source

Expanding molecular diagnostic capacity for COVID-19 in Ethiopia: operational implications, challenges and lessons learnt

open access: yesThe Pan African Medical Journal, 2021
Efforts towards slowing down coronavirus (COVID-19) transmission and reducing mortality have focused on timely case detection, isolation and treatment.
Adisu Kebede   +17 more
doaj   +1 more source

Reverse-Transcriptase Polymerase Chain Reaction to Detect Extracellular mRNAs [PDF]

open access: yes, 2011
The presence of extracellular nucleic acids has been reported in serum/plasma from cancer and diabetes patients that may help in disease diagnosis. Taking insulin-producing cells as examples here, RT-PCR was used to investigate a correlation between the presence and amounts of extracellular mRNA(s) and cell mass and/or function. RT-PCR was performed on
Sweta, Rani, Lorraine, O'Driscoll
openaire   +4 more sources

Capturing the ‘ome’ : the expanding molecular toolbox for RNA and DNA library construction [PDF]

open access: yes, 2018
All sequencing experiments and most functional genomics screens rely on the generation of libraries to comprehensively capture pools of targeted sequences.
Boone, Morgane   +2 more
core   +2 more sources

A semi-quantitative RT-PCR method to measure the in vivo effect of dietary conjugated linoleic acid on porcine muscle PPAR gene expression

open access: yesBiological Procedures Online, 2003
Conjugated linoleic acid (CLA) can activate (in vitro) the nuclear transcription factors known as the peroxisome proliferators activated receptors (PPAR).
Meadus W.J.
doaj   +1 more source

Capacity of rTth polymerase to detect RNA in the presence of various inhibitors. [PDF]

open access: yesPLoS ONE, 2018
The full potential of the real-time reverse transcription polymerase chain reaction (RT-PCR) as a rapid and accurate diagnostic method is limited by DNA polymerase inhibitors as well as reverse transcriptase inhibitors which are ubiquitous in clinical ...
Dongyang Cai   +4 more
doaj   +1 more source

The polymerase chain reaction-based SARS-CoV-2 detection

open access: yesUniversidad Médica Pinareña, 2020
Introduction: the reverse transcriptase polymerase chain reaction is a highly precise technique in the detection and amplification of genetic material, and therefore in the detection of viruses.
José Francisco Cancino-Mesa   +2 more
doaj   +2 more sources

SARS-CoV-2 RNA detection in cerebrospinal fluid: Presentation of two cases and review of literature

open access: yesBrain, Behavior, & Immunity - Health, 2021
Neurological manifestations of SARS-CoV-2 infection are multiple and heterogeneous. However, confirmation of nervous system impairment by viral RNA detection in cerebrospinal fluid (CSF) is uncommon.
María Belén Luis   +3 more
doaj   +1 more source

REVERSE TRANSCRIPTASE-POLYMERASE CHAIN REACTION ASSAYS FOR PROSTATE CANCER

open access: yesUrologic Clinics of North America, 1997
RT-PCR, if targeted against a prostate-specific marker, can be a highly specific and sensitive assay to detect the presence of occult prostate cancer cells at sites far distant from the primary tumor. The low false-positive rate (0.8%) observed when combining most published studies and the high rate of detection of prostate cells in metastatic patients
C A, Olsson   +3 more
openaire   +2 more sources

The mazEF toxin-antitoxin system as a novel antibacterial target in Acinetobacter baumannii [PDF]

open access: yes, 2014
Although analysis of toxin-antitoxin (TA) systems can be instructive, to date, there is no information on the prevalence and identity of TA systems based on a large panel of Acinetobacter baumannii clinical isolates.
Ghafourian, S   +6 more
core   +5 more sources

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