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What are we missing? Three cases of severe COVID-19 pneumonia with negative testing
The COVID-19 pandemic has drastically affected health care systems globally. Reverse transcriptase-polymerase chain reaction is currently the preferred method of detecting COVID-19; however, sensitivity of this test remains questionable.
Aisha Siddiqui +5 more
doaj +1 more source
Efforts towards slowing down coronavirus (COVID-19) transmission and reducing mortality have focused on timely case detection, isolation and treatment.
Adisu Kebede +17 more
doaj +1 more source
Reverse-Transcriptase Polymerase Chain Reaction to Detect Extracellular mRNAs [PDF]
The presence of extracellular nucleic acids has been reported in serum/plasma from cancer and diabetes patients that may help in disease diagnosis. Taking insulin-producing cells as examples here, RT-PCR was used to investigate a correlation between the presence and amounts of extracellular mRNA(s) and cell mass and/or function. RT-PCR was performed on
Sweta, Rani, Lorraine, O'Driscoll
openaire +4 more sources
Capturing the ‘ome’ : the expanding molecular toolbox for RNA and DNA library construction [PDF]
All sequencing experiments and most functional genomics screens rely on the generation of libraries to comprehensively capture pools of targeted sequences.
Boone, Morgane +2 more
core +2 more sources
Conjugated linoleic acid (CLA) can activate (in vitro) the nuclear transcription factors known as the peroxisome proliferators activated receptors (PPAR).
Meadus W.J.
doaj +1 more source
Capacity of rTth polymerase to detect RNA in the presence of various inhibitors. [PDF]
The full potential of the real-time reverse transcription polymerase chain reaction (RT-PCR) as a rapid and accurate diagnostic method is limited by DNA polymerase inhibitors as well as reverse transcriptase inhibitors which are ubiquitous in clinical ...
Dongyang Cai +4 more
doaj +1 more source
The polymerase chain reaction-based SARS-CoV-2 detection
Introduction: the reverse transcriptase polymerase chain reaction is a highly precise technique in the detection and amplification of genetic material, and therefore in the detection of viruses.
José Francisco Cancino-Mesa +2 more
doaj +2 more sources
SARS-CoV-2 RNA detection in cerebrospinal fluid: Presentation of two cases and review of literature
Neurological manifestations of SARS-CoV-2 infection are multiple and heterogeneous. However, confirmation of nervous system impairment by viral RNA detection in cerebrospinal fluid (CSF) is uncommon.
María Belén Luis +3 more
doaj +1 more source
REVERSE TRANSCRIPTASE-POLYMERASE CHAIN REACTION ASSAYS FOR PROSTATE CANCER
RT-PCR, if targeted against a prostate-specific marker, can be a highly specific and sensitive assay to detect the presence of occult prostate cancer cells at sites far distant from the primary tumor. The low false-positive rate (0.8%) observed when combining most published studies and the high rate of detection of prostate cells in metastatic patients
C A, Olsson +3 more
openaire +2 more sources
The mazEF toxin-antitoxin system as a novel antibacterial target in Acinetobacter baumannii [PDF]
Although analysis of toxin-antitoxin (TA) systems can be instructive, to date, there is no information on the prevalence and identity of TA systems based on a large panel of Acinetobacter baumannii clinical isolates.
Ghafourian, S +6 more
core +5 more sources

