Results 21 to 30 of about 172,121 (249)

Expanding molecular diagnostic capacity for COVID-19 in Ethiopia: operational implications, challenges and lessons learnt

The Pan African Medical Journal, 2021
Efforts towards slowing down coronavirus (COVID-19) transmission and reducing mortality have focused on timely case detection, isolation and treatment.
Adisu Kebede, Betty Lanyero, Berhane Beyene, Mayur Lalji Mandalia, Daniel Melese, Feven Girmachew, Adamu Mekonnen, Gonfa Ayana, Nebiyou Yemanebirhan, Getnet Hailu, Habtamu Asrat, Negash Nurahmed, Andargachew Gashu, Kirubel Eshetu, Zewdu Assefa, Aschalew Abayneh, Emmanuel Musa, Ebba Abate   +17 more
doaj   +1 more source

REVERSE TRANSCRIPTASE-POLYMERASE CHAIN REACTION ASSAYS FOR PROSTATE CANCER

Urologic Clinics of North America, 1997
RT-PCR, if targeted against a prostate-specific marker, can be a highly specific and sensitive assay to detect the presence of occult prostate cancer cells at sites far distant from the primary tumor. The low false-positive rate (0.8%) observed when combining most published studies and the high rate of detection of prostate cells in metastatic patients
C A, Olsson, G M, de Vries, R, Buttyan, A E, Katz   +3 more
openaire   +2 more sources

The polymerase chain reaction-based SARS-CoV-2 detection

Universidad Médica Pinareña, 2020
Introduction: the reverse transcriptase polymerase chain reaction is a highly precise technique in the detection and amplification of genetic material, and therefore in the detection of viruses.
José Francisco Cancino-Mesa, Adrián Alejandro Vitón-Castillo, Jorge Casí-Torres   +2 more
doaj   +2 more sources

Molecularly imprinted nanoparticles for inhibiting ribonuclease in reverse transcriptase polymerase chain reaction [PDF]

The Analyst, 2018
We showed that molecularly imprinted nanoparticles (nanoMIPs) could efficiently inhibit the activities of the RNase in RT-PCR reactions, demonstrating that the tailor-made nanomaterials are very promising for use in routine biological assays.
Xiaotong Feng, Jon Ashley, Tongchang Zhou, Arnab Halder, Yi Sun   +4 more
openaire   +6 more sources

Enteroviral pharyngitis diagnosed by reverse transcriptase-polymerase chain reaction. [PDF]

Archives of Disease in Childhood, 1996
The role of enteroviruses in childhood pharyngitis was investigated using enteroviral specific reverse transcriptase-polymerase chain reaction (RT-PCR). Viral/bacterial throat swabs were taken from 50 children with acute pharyngitis and 26 controls.
M, Sharland, J, Hodgson, E G, Davies, J, Booth, S, Jeffery   +4 more
openaire   +3 more sources

Capacity of rTth polymerase to detect RNA in the presence of various inhibitors. [PDF]

PLoS ONE, 2018
The full potential of the real-time reverse transcription polymerase chain reaction (RT-PCR) as a rapid and accurate diagnostic method is limited by DNA polymerase inhibitors as well as reverse transcriptase inhibitors which are ubiquitous in clinical ...
Dongyang Cai, Ole Behrmann, Frank Hufert, Gregory Dame, Gerald Urban   +4 more
doaj   +1 more source

Comparative analysis and identification of spike mutations in Iranian COVID-19 samples from the first three waves of disease

Advanced Biomedical Research, 2023
Background: The spike surface glycoprotein of SARS-CoV-2 is the essential protein in virus attachment to the target cell and cell entrance. As this protein contains immunodominant epitopes and is the main target for immune recognition, it is the critical
Parisa Shoaei, Mohammad M Ranjbar, Samaneh Tokhanbigli, Behrouz Ataei, Abbas Alibakhshi, Shaghayegh Haghjooy Javanmard, Shahrzad Ahangarzadeh   +6 more
doaj   +1 more source

Fetal Cytokine Expression In UteroDetected by Reverse Transcriptase Polymerase Chain Reaction [PDF]

Pediatric Research, 1995
Cytokine expression at the maternal-fetal interface is well documented. Some authors have postulated the existence of a bidirectional cytokine signaling mechanism that is critical to the maintenance of normal pregnancy. The role of the fetus (versus fetally derived placental tissues) in this process is unknown.
J N, Jarvis, L, Deng, S M, Berry, R, Romero, H, Moore   +4 more
openaire   +2 more sources

RIPK3 dampens mitochondrial bioenergetics and lipid droplet dynamics in metabolic liver disease

Hepatology, EarlyView., 2022
RIPK3 dampens mitochondrial bioenergetics and lipid droplet dynamics in metabolic liver disease. Abstract Background and Aims Receptor‐interacting protein kinase 3 (RIPK3) mediates NAFLD progression, but its metabolic function is unclear. Here, we aimed to investigate the role of RIPK3 in modulating mitochondria function, coupled with lipid droplet (LD)
Marta B. Afonso, Tawhidul Islam, Julie Magusto, Ricardo Amorim, Véronique Lenoir, Rui F. Simões, José Teixeira, Liana C. Silva, Dominique Wendum, Isabelle Jéru, Corinne Vigouroux, Rui E. Castro, Paulo J. Oliveira, Carina Prip‐Buus, Vlad Ratziu, Jérémie Gautheron, Cecília M. P. Rodrigues   +16 more
wiley   +1 more source

SARS-CoV-2 RNA detection in cerebrospinal fluid: Presentation of two cases and review of literature

Brain, Behavior, & Immunity - Health, 2021
Neurological manifestations of SARS-CoV-2 infection are multiple and heterogeneous. However, confirmation of nervous system impairment by viral RNA detection in cerebrospinal fluid (CSF) is uncommon.
María Belén Luis, Nora Fernández Liguori, Pablo Adrián López, Ricardo Alonso   +3 more
doaj   +1 more source