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Methods and ProtocolsReverse transcription polymerase chain reaction (RT-PCR) is an important tool for the detection of target RNA molecules and the assay of RNA pathogens.
Evgeniya V. Smirnova +5 more
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The comparison of chest CT and RT-PCR during the diagnosis of COVID-19 [PDF]
Ķazaķstannyṇ Klinikalyķ Medicinasy, 2021 Objective: Our aim in this study is to compare the diagnostic accuracy of chest computed tomography and Real-time Reverse Transcriptase Polymerase Chain Reaction in a population with suspected Coronavirus Disease 2019 (COVID-19) presenting at the ...
Avni Uygar Seyhan +5 more
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A Recent Non cytopathogenic bovine viral diarrhea virus detected by immunofluorescence and reverse transcriptase polymerase chain reaction [PDF]
Benha Veterinary Medical Journal, 2021 In the present study, a non-cytopathogenic bovine viral diarrhea virus (BVDV) field strains from bovine semen samples were detected by immunofluorescence (IF) and reverse transcriptase polymerase chain reaction (RT-PCR).
Ehab ElNahas +3 more
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Experience With Pretravel Testing for SARS-CoV-2 at an Academic Medical Center
Academic Pathology, 2021 International travel has been a significant factor in the coronavirus disease 2019 pandemic. Many countries and airlines have implemented travel restrictions to limit the spread of the causative agent, severe acute respiratory syndrome coronavirus-2.
Katherine L. Imborek MD +5 more
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Direct in situ reverse transcriptase-polymerase chain reaction [PDF]
American Journal of Physiology-Cell Physiology, 2001 In situ hybridization has been used for localization of specific nucleic acid sequences at the cellular level despite providing relatively low-detection sensitivity. In situ reverse transcriptase-polymerase chain reactions (RT-PCR) enhance sensitivity and thus enable localization of low-abundance mRNA in a cell.
R, Kher, R, Bacallao
openaire +2 more sources
Pakistan Armed Forces Medical Journal, 2022 Objective: To evaluate the accuracy of the COVID-19 Ag Rapid test device for detection of SARS-CoV-2 with Reverse Transcriptase Polymerase Chain Reaction as the gold standard. Study Design: Cross-sectional study.
Noman Shakoor +5 more
doaj +1 more source
Long term persistence of coronavirus SARS-CoV-2 infection
Einstein (São Paulo), 2021 During the COVID-19 pandemic, a case of a long-term persistence of SARS-CoV-2 infection (from March 26 to May 20, 2020) was identified at a private hospital in São Paulo, SP, Brazil.
João Renato Rebello Pinho +7 more
doaj +2 more sources
Acute viral gastroenteritis in children hospitalized in Iksan, Korea during December 2010-June 2011 [PDF]
Korean Journal of Pediatrics, 2013 PurposeViral etiology is common in cases of children with acute diarrhea, and antibiotic therapy is usually not required. Therefore, it is important to determine the distribution of common viruses among children hospitalized with acute diarrhea.MethodsWe
Cheol Whoan So +4 more
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Detection of chicken astrovirus by reverse transcriptase-polymerase chain reaction [PDF]
Avian Pathology, 2009 The development of a reverse transcriptase-polymerase chain reaction (RT-PCR) test for detecting chicken astroviruses (CAstV) is described. Primers, which amplified a fragment of 510 base pairs, were located in conserved regions within the ORF 1b (RNA polymerase) gene. The limit of detection of the test was estimated to be approximately 60 viral copies
V J, Smyth +3 more
openaire +2 more sources
Metasin: An Intra-Operative Real-Time Quantitative Reverse Transcription Polymerase-Chain Reaction (RTqPCR) Assay to Detect Metastatic Breast Cancer in Sentinel Lymph Nodes [PDF]
, 2014 The copyright of this thesis rests with the author and no quotation from it or information derived from it may be published without the prior written consent of the authorThe most important prognostic factor in breast cancer is the presence or absence of
Al-Ramadhani, Salma
core +4 more sources