Results 1 to 10 of about 193,159 (265)
A spontaneous termination mechanism of RNA polymerase V shapes the DNA methylation landscape in plants [PDF]
The EMBO JournalDNA methylation plays critical roles in eukaryotic gene silencing, genome imprinting, viral defense, and suppression of transposable elements. In plants, RNA Polymerase V (Pol V)-generated non-coding RNA guides DNA methylation through the RNA-directed ...
Guohui Xie +15 more
doaj +2 more sources
Polymerase IV occupancy at RNA-directed DNA methylation sites requires SHH1 [PDF]
Nature, 2013 DNA methylation is an epigenetic modification that plays critical roles in gene silencing, development, and genome integrity. In Arabidopsis, DNA methylation is established by DOMAINS REARRANGED METHYLTRANSFERASE 2 (DRM2) and targeted by 24 nt small interfering RNAs (siRNAs) through a pathway termed RNA-directed DNA methylation (RdDM)1.
Julie A Law +2 more
exaly +3 more sources
RNA-mediated in vivo Directed Evolution in Yeast
Bio-Protocol, 2022 Directed evolution is a powerful approach to obtain genetically-encoded sought-for traits. Compared to the prolonged adaptation regimes to mutations occurring under natural selection, directed evolution unlocks rapid screening and selection of mutants ...
Emil Jensen, Michael Jensen
doaj +1 more source
RNA INTERFERNCE (RNAI) FOR SOME GENES FROM BABESIA BOVIS [PDF]
Research Journal of Applied Biotechnology, 2016 Babesia parasites are responsible for enormous economic losses to the livestock industry worldwide. There is no vaccine available to protect from the infection.
Ikuo Igarashi +2 more
doaj +1 more source
eLife, 2022 In plants, selfish genetic elements, including retrotransposons and DNA viruses, are transcriptionally silenced by RNA-directed DNA methylation. Guiding the process are short interfering RNAs (siRNAs) cut by DICER-LIKE 3 (DCL3) from double-stranded ...
Andrew Loffer +5 more
doaj +1 more source
SHH1, a homeodomain protein required for DNA methylation, as well as RDR2, RDM4, and chromatin remodeling factors, associate with RNA polymerase IV. [PDF]
PLoS Genetics, 2011 DNA methylation is an evolutionarily conserved epigenetic modification that is critical for gene silencing and the maintenance of genome integrity. In Arabidopsis thaliana, the de novo DNA methyltransferase, domains rearranged methyltransferase 2 (DRM2),
Julie A Law +3 more
doaj +1 more source
Animals, 2023 The genus Capripoxvirus belongs to the Poxviridae family. The sheeppox, goatpox, and lumpy skin disease viruses are three species of this genus with 96% identity in their genomes.
Ghulam Mustafa +6 more
doaj +1 more source
Human Leukemic Cells: RNA‐Directed DNA Polymerase [PDF]
European Journal of Biochemistry, 1974 The enzyme(s) RNA‐dependent DNA polymerase (reverse transcriptase) has been isolated from human lymphocytic cell lines which have been derived from patients with leukemia, infectious mononucleosis, and from normal healthy donors. The reverse transcriptase was purified through high‐speed glycerol gradients, and by DEAE‐cellulose and phosphocellulose ...
L S, Desai +3 more
openaire +2 more sources
Metal A and metal B sites of nuclear RNA polymerases Pol IV and Pol V are required for siRNA-dependent DNA methylation and gene silencing. [PDF]
PLoS ONE, 2009 Plants are unique among eukaryotes in having five multi-subunit nuclear RNA polymerases: the ubiquitous RNA polymerases I, II and III plus two plant-specific activities, nuclear RNA polymerases IV and V (previously known as Polymerases IVa and IVb).
Jeremy R Haag +2 more
doaj +1 more source
RNA-Directed DNA Polymerase and Virus-Induced Leukemia in Mice [PDF]
Proceedings of the National Academy of Sciences, 1973 Rifamycin antibiotics, which inhibit RNA-directed DNA polymerase of Rauscher leuckemia virus, prevent the leukemogenic activity of the virus, and the effect of leukemogenesis correlates with the magnitude of inhibition of the purified enzyme.
A M, Wu, R C, Ting, R C, Gallo
openaire +2 more sources