Results 31 to 40 of about 193,159 (265)
Characterization of an RNA-directed DNA polymerase found in association with murine intracytoplasmic A-particles [PDF]
Journal of Virology, 1977 An RNA-directed DNA polymerase was found to be associated with intracytoplasmic A-particles from DBA/2 mouse leukemia cells. The enzyme activity was detected after disrupting the purified particles with 2 M NaCl-20 mM dithiothreitol. The presence of a divalent cation and all four deoxyribonucleoside triphosphates was essential for this enzyme activity.
Kohno, M, Tanaka, H
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On the origin of DNA genomes: evolution of the division of labor between template and catalyst in model replicator systems. [PDF]
PLoS Computational Biology, 2011 The division of labor between template and catalyst is a fundamental property of all living systems: DNA stores genetic information whereas proteins function as catalysts. The RNA world hypothesis, however, posits that, at the earlier stages of evolution,
Nobuto Takeuchi +2 more
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Characterization of endogenous RNA-directed DNA polymerase activity of reticuloendotheliosis viruses [PDF]
Journal of Virology, 1975 Reticuloendotheliosis viruses (REV) contain an endogenous RNA-directed DNA polymerase activity. The endogenous DNA polymerase activity can be elicited in purified preparations of REV by treatment with nonionic detergents. The enzyme activity has a strong preference for manganous ions.
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A Protein Complex Required for Polymerase V Transcripts and RNA- Directed DNA Methylation in Arabidopsis [PDF]
Current Biology, 2010 DNA methylation is an epigenetic modification associated with gene silencing. In Arabidopsis, DNA methylation is established by DOMAINS REARRANGED METHYLTRANSFERASE 2 (DRM2), which is targeted by small interfering RNAs through a pathway termed RNA-directed DNA methylation (RdDM).
Law, Julie A. +6 more
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PLoS Genetics, 2020 RNA-directed DNA methylation (RdDM) is a biological process in which non-coding RNA molecules direct the addition of DNA methylation to specific DNA sequences.
Robert M Erdmann, Colette L Picard
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PLoS Pathogens, 2022 ASFV is a large DNA virus that is highly pathogenic in domestic pigs. How this virus is sensed by the innate immune system as well as why it is so virulent remains enigmatic.
Yong Ran +10 more
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Human placentas contain a specific inhibitor of RNA-directed DNA polymerase. [PDF]
Proceedings of the National Academy of Sciences, 1981 Human placental extracts contain a specific inhibitor of mammalian retroviral RNA-directed DNA polymerase (deoxynucleosidetriphosphate:DNA deoxynucleotidyltransferase, EC 2.7.7.7) activity. This inhibitor copurifies with retrovirus-like particles in human placental extracts.
J A, Nelson, J A, Levy, J C, Leong
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T4 DNA polymerase improves the efficiency of multiple site-directed mutagenesis
Biotechnology & Biotechnological Equipment, 2016 Site-directed mutagenesis (SDM) is a useful tool to study the functions of regulatory sequences of DNA and RNA, and the structures and functions of proteins. Numerous methods have been developed for either single or multiple SDM (MSDM).
Wei Guo +4 more
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Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation
FEBS Letters, EarlyView.Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe +3 more
wiley +1 more source
Molecular Systems Biology, 2014 DNA replication, transcription and repair involve the recruitment of protein complexes that change their composition as they progress along the genome in a directed or strand‐specific manner.
Benedikt Zacher +4 more
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