Optimizing the specificity of nucleic acid hybridization. [PDF]
Nat Chem, 2012 The specific hybridization of complementary sequences is an essential property of nucleic acids, enabling diverse biological and biotechnological reactions and functions. However, the specificity of nucleic acid hybridization is compromised for long strands, except near the melting temperature. Here, we analytically derived the thermodynamic properties
Zhang DY, Chen SX, Yin P.
europepmc +7 more sources
Continuously tunable nucleic acid hybridization probes. [PDF]
Nat Methods, 2015 In silico-designed nucleic acid probes and primers often do not achieve favorable specificity and sensitivity tradeoffs on the first try, and iterative empirical sequence-based optimization is needed, particularly in multiplexed assays. We present a novel, on-the-fly method of tuning probe affinity and selectivity by adjusting the stoichiometry of ...
Wu LR +10 more
europepmc +6 more sources
A quantitative model predicts how m<sup>6</sup>A reshapes the kinetic landscape of nucleic acid hybridization and conformational transitions. [PDF]
Nat Commun, 2021 N6-methyladenosine (m6A) is a post-transcriptional modification that controls gene expression by recruiting proteins to RNA sites. The modification also slows biochemical processes through mechanisms that are not understood.
Liu B +8 more
europepmc +2 more sources
Nucleic Acid Hybridization [PDF]
Definitions, 2020 Abbreviations -- Preface -- 1. Introduction to nucleic acid hybridization -- Structure of nucleic acids -- Forces stabilizing DNA structure -- Mismatched base pairs -- Stability of nucleic acids -- Effects of pH -- Effects of temperature -- Renaturation and hybridization -- 2.
M. Anderson
semanticscholar +4 more sources
Surface Micro-Patterned Biofunctionalized Hydrogel for Direct Nucleic Acid Hybridization Detection [PDF]
Biosensors, 2023 The present research is focused on the development of a biofunctionalized hydrogel with a surface diffractive micropattern as a label-free biosensing platform.
Paola Zezza +4 more
doaj +2 more sources
Chemical Architecture and Applications of Nucleic Acid Derivatives Containing 1,2,3-Triazole Functionalities Synthesized via Click Chemistry [PDF]
Molecules, 2012 There is considerable attention directed at chemically modifying nucleic acids with robust functional groups in order to alter their properties. Since the breakthrough of copper-assisted azide-alkyne cycloadditions (CuAAC), there have been several ...
Wei Gong +2 more
doaj +3 more sources
Effect of Native Gastric Mucus on in vivo Hybridization Therapies Directed at Helicobacter pylori [PDF]
Molecular Therapy: Nucleic Acids, 2015 Helicobacter pylori infects more than 50% of the worldwide population. It is mostly found deep in the gastric mucus lining of the stomach, being a major cause of peptic ulcers and gastric adenocarcinoma. To face the increasing resistance of H.
Rita S Santos +13 more
doaj +8 more sources
Nucleic acid nanoassembly-enhanced RNA therapeutics and diagnosis
Acta Pharmaceutica Sinica B, 2023 RNAs are involved in the crucial processes of disease progression and have emerged as powerful therapeutic targets and diagnostic biomarkers. However, efficient delivery of therapeutic RNA to the targeted location and precise detection of RNA markers ...
Mengnan Zhao +8 more
doaj +2 more sources
Activation of enzymatic catalysis via nucleic acid hybridization affords synergistic coupling of specificity, potency, and signal amplification [PDF]
Nature CommunicationsIn this work, we establish the use of nucleic acid hybridization to program the activation of catalysis in enzymes. We do this via “thiol switching”, using thiolated oligonucleotides: a protein is inactivated by conjugation to an oligonucleotide via a ...
Mireia Casanovas Montasell +4 more
doaj +2 more sources
Label-Free Potentiometry for Detecting DNA Hybridization Using Peptide Nucleic Acid and DNA Probes [PDF]
Sensors, 2013 Peptide nucleic acid (PNA) has outstanding affinity over DNA for complementary nucleic acid sequences by forming a PNA-DNA heterodimer upon hybridization via Watson-Crick base-pairing.
Yuji Miyahara +6 more
doaj +2 more sources