Results 1 to 10 of about 115,376 (162)

Surface Micro-Patterned Biofunctionalized Hydrogel for Direct Nucleic Acid Hybridization Detection [PDF]

Biosensors, 2023
The present research is focused on the development of a biofunctionalized hydrogel with a surface diffractive micropattern as a label-free biosensing platform.
Paola Zezza, María Isabel Lucío, Estrella Fernández, Ángel Maquieira, María-José Bañuls   +4 more
doaj   +2 more sources

Continuously tunable nucleic acid hybridization probes. [PDF]

Nat Methods, 2015
In silico-designed nucleic acid probes and primers often do not achieve favorable specificity and sensitivity tradeoffs on the first try, and iterative empirical sequence-based optimization is needed, particularly in multiplexed assays. We present a novel, on-the-fly method of tuning probe affinity and selectivity by adjusting the stoichiometry of ...
Wu LR, Wang JS, Fang JZ, Evans ER, Pinto A, Pekker I, Boykin R, Ngouenet C, Webster PJ, Beechem J, Zhang DY.   +10 more
europepmc   +5 more sources

Optimizing the specificity of nucleic acid hybridization. [PDF]

Nat Chem, 2012
The specific hybridization of complementary sequences is an essential property of nucleic acids, enabling diverse biological and biotechnological reactions and functions. However, the specificity of nucleic acid hybridization is compromised for long strands, except near the melting temperature. Here, we analytically derived the thermodynamic properties
Zhang DY, Chen SX, Yin P.
europepmc   +4 more sources

Activation of enzymatic catalysis via nucleic acid hybridization affords synergistic coupling of specificity, potency, and signal amplification [PDF]

Nature Communications
In this work, we establish the use of nucleic acid hybridization to program the activation of catalysis in enzymes. We do this via “thiol switching”, using thiolated oligonucleotides: a protein is inactivated by conjugation to an oligonucleotide via a ...
Mireia Casanovas Montasell, Lou M. V. Raeven, Mathilde Ravn Malm, Jan C. M. van Hest, Alexander N. Zelikin   +4 more
doaj   +2 more sources

Colorimetric Detection of Ehrlichia Canis via Nucleic Acid Hybridization in Gold Nano-Colloids [PDF]

Sensors, 2014
Canine monocytic ehrlichiosis (CME) is a major thick-bone disease of dog caused by Ehrlichia canis. Detection of this causal agent outside the laboratory using conventional methods is not effective enough. Thus an assay for E.
Ajima Muangchuen, Piyasak Chaumpluk, Annop Suriyasomboon, Sanong Ekgasit   +3 more
doaj   +2 more sources

In-situ dual-lighting system for HER2 nucleic acid and protein co-localization through simultaneous visual signal amplification in single pathological sections [PDF]

Communications Medicine
Background The pathological state of human epidermal growth factor receptor-2 (HER2) determines the treatment strategies. Immunohistochemistry (IHC) and in situ hybridization (ISH) are the only two recognized diagnostic methods for HER2-positive (HER2 + )
Tiantian Wang, Zhiqing Chen, Xuan Fu, Lin Chen, Yao Huang, Ang Liu, Qingling Zhang, Bei Hu, Weiyang Liang, Qunyue Wu, Shan Li, Xin Li, Lei Zhang   +12 more
doaj   +2 more sources

Nucleic acid amphiphiles: Synthesis, properties, and applications

Molecular Therapy: Nucleic Acids, 2023
Nucleic acid amphiphiles, referring to nucleic acids modified with large hydrophobic groups, have been widely used in programmable bioengineering. Since nucleic acids are intrinsically hydrophilic, the hydrophobic groups endow nucleic acid amphiphiles ...
Amu Gubu, Xueli Zhang, Aiping Lu, Baoting Zhang, Yuan Ma, Ge Zhang   +5 more
doaj   +1 more source

Fast and non-toxic in situ hybridization without blocking of repetitive sequences. [PDF]

PLoS ONE, 2012
Formamide is the preferred solvent to lower the melting point and annealing temperature of nucleic acid strands in in situ hybridization (ISH). A key benefit of formamide is better preservation of morphology due to a lower incubation temperature. However,
Steen H Matthiesen, Charles M Hansen
doaj   +1 more source

Developing predictive hybridization models for phosphorothioate oligonucleotides using high-resolution melting.

PLoS ONE, 2022
The ability to predict nucleic acid hybridization energies has been greatly enabling for many applications, but predictive models require painstaking experimentation, which may limit expansion to non-natural nucleic acid analogues and chemistries.
Siyuan S Wang, Erhu Xiong, Sanchita Bhadra, Andrew D Ellington   +3 more
doaj   +1 more source

Application of Graphene FET Nucleic Acid Biosensor in Human Motion Measurement [PDF]

International Journal Bioautomation, 2018
In order to explore the application of graphene field effect transistor (FET) nucleic acid biosensor, the sensitivity and selectivity of nano FET biosensor detection are improved by the large physical surface ratio, high electron mobility, excellent ...
Hang Yin
doaj   +1 more source