Results 41 to 50 of about 119,828 (299)

Nucleic acid analysis by sandwich hybridization

Journal of Clinical Laboratory Analysis, 1989
AbstractOne of the most significant achievements of the biochemist during the past two decades is the use to which immunologically based assays have been put in clinical diagnosis (Hood et al.: Immunology, 1984). The problem faced and surmounted by immunologists in effecting the transition from research tool to routine clinical assay bears a remarkable
P J, Nicholls, A D, Malcolm
openaire   +2 more sources

Dealing with the problem of non-specific in situ mRNA hybridization signals associated with plant tissues undergoing programmed cell death

Plant Methods, 2010
Background In situ hybridization is a general molecular method typically used for the localization of mRNA transcripts in plants. The method provides a valuable tool to unravel the connection between gene expression and anatomy, especially in species ...
Jokela Anne, Kestilä Johanna, Sääskilahti Mira, Sutela Suvi, Vuosku Jaana, Sarjala Tytti, Häggman Hely   +6 more
doaj   +1 more source

Building biologically active nucleic acid nanocomplexes

, 2008
The Bioplex technology allows the hybridization of functional entities to various forms of nucleic acids by the use of synthetic nucleic acid analogs.
Svahn, MG, Wenska, M, Lundin, KE, Strömberg, R,, Simonson, OE, Moreno, PMD, Smith, CIE   +6 more
core   +1 more source

Three phosphatase families form a community: The phosphohydrolases that act upon inositol pyrophosphates

FEBS Letters, EarlyView.
Inositol pyrophosphates are energy‐rich signaling molecules that perform critical functions in cells. Three different families of phosphatases hydrolyze the β phosphate of the inositol pyrophosphate molecules: two have narrow specificities and one is promiscuous.
Ronda J. Rolfes
wiley   +1 more source

Hybridization properties of immobilized nucleic acids

Nucleic Acids Research, 1987
The 5'-end attachment of oligonucleotides to dextran supports facilitates the study of the hybridization properties of an immobilized oligonucleotide system. The hybridization properties which were studied include: hybridization capacity and kinetics, hybridization-complex stability, and reagents influencing hybridization efficiency.
T. R. Gingeras, D. Y. Kwoh, G. R. Davis
openaire   +3 more sources

UiO‐66 metal–organic frameworks in biomedicine: From structural tunability to bioimaging, photodiagnostics, and photodynamic cancer therapy

FEBS Open Bio, EarlyView.
UiO‐66(Zr) metal–organic frameworks are chemically stable, biocompatible, and highly tunable nanomaterials. Their modular structure enables controlled drug delivery, multimodal bioimaging, and light‐activated photodynamic therapy, supporting integrated diagnostic and therapeutic (theranostic) applications in cancer and biomedical research.
Veronika Huntošová, Grigorii Rakhalskii, Miroslav Almáši   +2 more
wiley   +1 more source

Delineation of individual human chromosomes in metaphase and interphase cells by in situ suppression hybridization using recombinant DNA libraries [PDF]

, 1988
A method of in situ hybridization for visualizing individual human chromosomes from pter to qter, both in metaphase spreads and interphase nuclei, is reported.
Cremer, Thomas, Manuelidis, L., Lichter, Peter, Borden, J., Ward, D. C.   +4 more
core   +1 more source

Blood Biomarkers and Surface‐Enhanced Raman Spectroscopy for Gout: A Comprehensive Review

Advanced Functional Materials, EarlyView.
Schematic illustrating gout disease progression from asymptomatic hyperuricemia to chronic tophaceous disease, highlighting the limitations of conventional imaging and biochemical diagnostics and the potential of engineered SERS platforms for ultrasensitive blood‐based detection of urate‐related biomarkers across disease stages, with the color gradient
Isuri Perera, Chiranjeevi Kanike, Appurva Tiwari, Hongyan Wu, Larry D. Unsworth, Ben Bin Xu, Xuehua Zhang   +6 more
wiley   +1 more source

Diagnosis of active tuberculosis disease: From microscopy to molecular techniques

Journal of Clinical Tuberculosis and Other Mycobacterial Diseases, 2016
Methods used for the laboratory diagnosis of tuberculosis are continually evolving in order to achieve more rapid, less expensive, and accurate results.
Adam J. Caulfield, Nancy L. Wengenack
doaj   +1 more source

Topological constraints in nucleic acid hybridization kinetics [PDF]

Nucleic Acids Research, 2005
A theoretical examination of kinetic mechanisms for forming knots and links in nucleic acid structures suggests that molecules involving base pairs between loops are likely to become topologically trapped in persistent frustrated states through the mechanism of 'helix-driven wrapping'. Augmentation of the state space to include both secondary structure
Bois, Justin S., Venkataraman, Suvir, Choi, Harry M. T., Spakowitz, Andrew J., Wang, Zhen-Gang, Pierce, Niles A.   +5 more
openaire   +3 more sources