Molecules, 2012 There is considerable attention directed at chemically modifying nucleic acids with robust functional groups in order to alter their properties. Since the breakthrough of copper-assisted azide-alkyne cycloadditions (CuAAC), there have been several ...
Wei Gong +2 more
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MethodsX, 2019 A fluorescence in situ hybridization (FISH) protocol was developed for nematodes in which nucleic acid probes are introduced within the organism via electroporation.
Casey L. Ruark-Seward +2 more
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New peptide nucleic acids for biotechnological applications [PDF]
, 2010 In the present study was designed and realized a new similar to the PNA. It is a chiral nucleo-γ-peptide, dabPNA, isomer of the aegPNA unit and characterized by a 2,4-diaminobutyric acid (DABA)-based backbone carrying the carboxymethylated nucleobase on ...
Castiglione, Mariangela
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A Widely Applicable Urea-based Fluorescent/Colorimetric mRNA in situ Hybridization Protocol
Bio-Protocol, 2019 In situ hybridization methods are routinely employed to detect nucleic acid sequences, allowing to localize gene expression or to study chromosomal organization in their native context. These methods rely on the pairwise binding of a labeled probe to the
Chiara Sinigaglia
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Locked nucleic acid pentamers as universal PCR primers for genomic DNA amplification. [PDF]
PLoS ONE, 2008 BACKGROUND: Multiplexing technologies, which allow for simultaneous detection of multiple nucleic acid sequences in a single reaction, can save a lot of time, cost and labor compared to traditional single reaction detection methods.
Zhen Sun +7 more
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Application of multiple response optimization design to quantum dot-encoded microsphere bioconjugates hybridization assay [PDF]
, 2011 The optimization of DNA hybridization for genotyping assays is a complex experimental problem that depends on multiple factors such as assay formats, fluorescent probes, target sequence, experimental conditions, and data analysis.
Morgan, Sarah L. +5 more
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Effect of Native Gastric Mucus on in vivo Hybridization Therapies Directed at Helicobacter pylori
Molecular Therapy: Nucleic Acids, 2015 Helicobacter pylori infects more than 50% of the worldwide population. It is mostly found deep in the gastric mucus lining of the stomach, being a major cause of peptic ulcers and gastric adenocarcinoma. To face the increasing resistance of H.
Rita S Santos +13 more
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Identification of single-point mutations in mycobacterial 16S rRNA sequences by confocal single-molecule fluorescence spectroscopy [PDF]
, 2006 Marme N, Friedrich A, Müller M, et al. Identification of single-point mutations in mycobacterial 16S rRNA sequences by confocal single-molecule fluorescence spectroscopy. Nucleic Acids Research.
Friedrich, Achim +7 more
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Penggunaan Pelacak DNA untuk Deteksi Papaya ringspot virus dengan Metode Hibridisasi Asam Nukleat
Jurnal Fitopatologi Indonesia, 2018 Use of DNA Probe for Detection of Papaya ringspot virus Using Nucleic Acid Hybridization MethodPapaya ringspot caused by Papaya ringspot virus (PRSV) is one of the most destructive diseases of papaya.
Irsan Nuhantoro +2 more
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Thermodynamic framework to assess low abundance DNA mutation detection by hybridization. [PDF]
PLoS ONE, 2017 The knowledge of genomic DNA variations in patient samples has a high and increasing value for human diagnostics in its broadest sense. Although many methods and sensors to detect or quantify these variations are available or under development, the ...
Hanny Willems +7 more
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