Results 31 to 40 of about 471,867 (378)

A method for nucleic acid hybridization to isolated chromosomes in suspension [PDF]

, 1987
A procedure was developed to provide differential fluorescent staining of metaphase chromosomes in suspension following nucleic acid hybridization. For this purpose metaphase chromosomes were isolated from a Chinese hamster x human hybrid cell line ...
Bier, Frank, Cremer, Christoph, Cremer, Thomas, Dudin, Gertrud, Hausmann, Michael, Schardin, Margit   +5 more
core   +1 more source

Furan-PNA : a mildly inducible irreversible interstrand crosslinking system targeting single and double stranded DNA [PDF]

, 2016
We here report on the design and synthesis of tailor-made furan-modified peptide nucleic acid (PNA) probes for covalent targeting of single stranded DNA through a crosslinking strategy.
Corradini, Roberto, Gyssels, Ellen, Madder, Annemieke, Manicardi, Alex   +3 more
core   +2 more sources

A CRISPR-Cas12a integrated SERS nanoplatform with chimeric DNA/RNA hairpin guide for ultrasensitive nucleic acid detection

Theranostics, 2022
Background: CRISPR-Cas12a has been integrated with nanomaterial-based optical techniques, such as surface-enhanced Raman scattering (SERS), to formulate a powerful amplification-free nucleic acid detection system.
Bohan Yin, Qin Zhang, Xinyu Xia, Chuanqi Li, Willis Kwun Hei Ho, Jia-xiang Yan, Yingying Huang, Honglian Wu, Pui Wang, C. Yi, J. Hao, Jianfang Wang, Honglin Chen, Siu Hong Dexter Wong, Mo Yang   +14 more
semanticscholar   +1 more source

A Study of the Detection of SARS-CoV-2 ORF1ab Gene by the Use of Electrochemiluminescent Biosensor Based on Dual-Probe Hybridization

Sensors, 2022
To satisfy the need to develop highly sensitive methods for detecting the severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) and further enhance detection efficiency and capability, a new method was created for detecting SARS-CoV-2 of the ...
Chunying Jiang, Xihui Mu, Shuai Liu, Zhiwei Liu, Bin Du, Jiang Wang, Jianjie Xu   +6 more
doaj   +1 more source

Electropermeabilization-based fluorescence in situ hybridization of whole-mount plant parasitic nematode specimens

MethodsX, 2019
A fluorescence in situ hybridization (FISH) protocol was developed for nematodes in which nucleic acid probes are introduced within the organism via electroporation.
Casey L. Ruark-Seward, Eric L. Davis, Tim L. Sit   +2 more
doaj   +1 more source

Detection of fluorescence in situ hybridization on human metaphase chromosomes by near-field scanning optical microscopy [PDF]

, 1995
Fluorescence in situ hybridization signals o­n human metaphase chromosomes are detected by a near-field scanning optical microscope. This makes it possible to localize and identify several fluorescently labeled genomic DNA fragments o­n a single ...
Jalocha, A., Jalocha, A., Moers, M.H.P., Moers, M.H.P., Ruiter, A.G.T., van Hulst, N.F.   +5 more
core   +3 more sources

A Widely Applicable Urea-based Fluorescent/Colorimetric mRNA in situ Hybridization Protocol

Bio-Protocol, 2019
In situ hybridization methods are routinely employed to detect nucleic acid sequences, allowing to localize gene expression or to study chromosomal organization in their native context. These methods rely on the pairwise binding of a labeled probe to the
Chiara Sinigaglia
doaj   +1 more source

Locked nucleic acid pentamers as universal PCR primers for genomic DNA amplification. [PDF]

PLoS ONE, 2008
BACKGROUND: Multiplexing technologies, which allow for simultaneous detection of multiple nucleic acid sequences in a single reaction, can save a lot of time, cost and labor compared to traditional single reaction detection methods.
Zhen Sun, Zhi Chen, Xiaoli Hou, Shuping Li, Haihong Zhu, Ji Qian, Daru Lu, Wei Liu   +7 more
doaj   +1 more source

Control of DNA Strand Displacement Kinetics Using Toehold Exchange [PDF]

, 2009
DNA is increasingly being used as the engineering material of choice for the construction of nanoscale circuits, structures, and motors. Many of these enzyme-free constructions function by DNA strand displacement reactions.
Winfree, Erik, Zhang, David Yu
core   +2 more sources

DNA‐Coated Upconversion Nanoparticles for Sensitive Nucleic Acid FRET Biosensing

Advanced Functional Materials, 2022
Despite the significant advancements of developing upconversion nanoparticles (UCNPs) for high performance biosensing and bioimaging, the development of DNA‐functionalized UCNPs with thin coatings, efficient surface passivation, and fully functional DNAs
Laura Francés-Soriano, Nestor Estébanez, J. Pérez‐Prieto, N. Hildebrandt   +3 more
semanticscholar   +1 more source