Results 31 to 40 of about 119,828 (299)

Influence of citrate buffer and flash heating in enhancing the sensitivity of ratiometric genosensing of Hepatitis C virus using plasmonic gold nanoparticles

open access: yesMicro and Nano Systems Letters, 2021
Gold nanoparticles (Au NPs) based technology has been shown to possess enormous potential in the viral nucleic acid diagnosis. Despite significant advancement in this domain, the existing literature reveals the diversity in the conditions employed for ...
Hrishikesh Shashi Prakash   +5 more
doaj   +1 more source

A Review of Peptide Nucleic Acid [PDF]

open access: yes, 2015
Peptide Nucleic Acid (PNA) is a nucleobase oligomer in which the whole backbone is mainly replaced by N-(2- aminoethyl) glycine units. PNA is considered as DNA with a neutral peptide backbone due to negative charged sugar–phosphate backbone.
Shafiquzzaman Siddiquee   +2 more
core   +1 more source

Detection of target DNA using fluorescent cationic polymer and peptide nucleic acid probes on solid support

open access: yesBMC Biotechnology, 2005
Background Nucleic acids detection using microarrays requires labelling of target nucleic acids with fluorophores or other reporter molecules prior to hybridization.
Leclerc Mario   +7 more
doaj   +1 more source

Comparative chromosome band mapping in primates byin situ suppression hybridization of band specific DNA microlibraries [PDF]

open access: yes, 1991
A DNA-library established from microdissected bands 8q23 to 8q24.1 of normal human chromosomes 8 (Lüdecke et al., 1989) was used as a probe for chromosomal in situ suppression (CISS-) hybridization to metaphase chromosomes of man and primates including ...
Cremer, Thomas   +4 more
core   +1 more source

Molecular beacons: nucleic acid hybridization and emerging applications [PDF]

open access: yes, 2001
Molecular beacons (MBs) are a novel class of nucleic acid probes that become fluorescent when bound to a complementary sequence. Because of this characteristic, coupled with the sequence specificity of nucleic acid hybridization and the sensitivity of ...
Antony, Thomas   +3 more
core   +2 more sources

Immobilon™-Ny+ Nucleic Acid Blotting Membrane: An Advanced Nylon Membrane Optimized for Superior Fixation and Reprobing

open access: yesBioTechniques, 1999
The use of charged nylon membranes in nucleic acid blotting applications has become an important factor in the success of hybridization-based assays. Retention of nucleic acids on these membranes is promoted by baking at 80°C under vacuum or by exposure ...
Michael A. Mansfield   +3 more
doaj   +1 more source

The Application of Hybridization Chain Reaction in the Detection of Foodborne Pathogens

open access: yesFoods, 2023
Today, with the globalization of the food trade progressing, food safety continues to warrant widespread attention. Foodborne diseases caused by contaminated food, including foodborne pathogens, seriously threaten public health and the economy.
Jinbin Zhao   +7 more
doaj   +1 more source

Fluorescence in situ hybridization of YAC clones after Alu-PCR amplification [PDF]

open access: yes, 1992
Alu-PCR protocols were optimized for the generation of human DNA probes from yeast strains containing yeast artificial chromosomes (YACs) with human inserts between 100 and 800 kb in size.
Cremer, Thomas   +2 more
core   +1 more source

Peach latent mosaic viroid in naturally infected sweet cherry trees in southern Italy

open access: yesInternational Journal of Horticultural Science, 2002
Peach latent mosaic viroid (PLMVd) was found in naturally infected sweet cherry trees grown in commercial orchards in southern Italy. The viroid was detected in nucleic acid extracts of symptomless leaves by molecular hybridization with a PLMVd cRNA ...
A. Crescenzi, A. Piazzolla, A. Hadidi
doaj   +1 more source

Detection of monosomy 7 in interphase cells of patients with myeloid disorders [PDF]

open access: yes, 1990
Six patients, five with acute myeloid leukemia (AML) and one with a myelodysplastic syndrome (MDS), were found to have monosomy 7 by conventional cytogenetics at diagnosis.
Cremer, Thomas   +5 more
core   +1 more source

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