Results 21 to 30 of about 115,619 (257)

Protein-Based Anchoring Methods for Nucleic Acid Detection in Lateral Flow Format Assays

Micromachines, 2023
The use of lateral flow assays to detect nucleic acid targets has many applications including point-of-care diagnostics, environmental monitoring, and food safety.
Kira Hallerbach, Khadijeh Khederlou, Lael Wentland, Lana Senten, Steven Brentano, Brian Keefe, Elain Fu   +6 more
doaj   +1 more source

Highly efficient and robust π-FISH rainbow for multiplexed in situ detection of diverse biomolecules

Nature Communications, 2023
Fluorescence in situ hybridization (FISH) methods with high sensitivity are needed. Here the authors develop multiplex πFISH rainbow to detect a range of biomolecules; they also combine this with the hybridization chain reaction to develop πFISH ...
Yingfeng Tao, Xiaoliu Zhou, Leqiang Sun, Da Lin, Huaiyuan Cai, Xi Chen, Wei Zhou, Bing Yang, Zhe Hu, Jing Yu, Jing Zhang, Xiaoqing Yang, Fang Yang, Bang Shen, Wenbao Qi, Zhenfang Fu, Jinxia Dai, Gang Cao   +17 more
doaj   +1 more source

Nucleic acid nanoassembly-enhanced RNA therapeutics and diagnosis

Acta Pharmaceutica Sinica B, 2023
RNAs are involved in the crucial processes of disease progression and have emerged as powerful therapeutic targets and diagnostic biomarkers. However, efficient delivery of therapeutic RNA to the targeted location and precise detection of RNA markers ...
Mengnan Zhao, Rujing Wang, Kunmeng Yang, Yuhong Jiang, Yachen Peng, Yuke Li, Zhen Zhang, Jianxun Ding, Sanjun Shi   +8 more
doaj   +1 more source

Nucleic Acid Analysis Using Multifunctional Hybridization Sensors

Proceedings, 2017
Hybridization of nucleic acid probes remains one of the most common strategies for sensing of [...]
Dmitry M. Kolpashchikov
doaj   +1 more source

Microfluidic Chip for the Electrochemical Detection of MicroRNAs: Methylene Blue Increasing the Specificity of the Biosensor

Frontiers in Chemistry, 2022
MicroRNAs (miRNAs) are biomarkers involved in biological processes that are released by cells and found in biological fluids such as blood. The development of nucleic acid-based biosensors has significantly increased in the past 10 years because the ...
Claire Poujouly, Jérémy Le Gall, Martina Freisa, Djamila Kechkeche, David Bouville, Jihed Khemir, Pedro Gonzalez-Losada, Jean Gamby   +7 more
doaj   +1 more source

Nucleic Acid Hybridization [PDF]

, 2020
Abbreviations -- Preface -- 1. Introduction to nucleic acid hybridization -- Structure of nucleic acids -- Forces stabilizing DNA structure -- Mismatched base pairs -- Stability of nucleic acids -- Effects of pH -- Effects of temperature -- Renaturation and hybridization -- 2.
openaire   +3 more sources

A Study of the Detection of SARS-CoV-2 ORF1ab Gene by the Use of Electrochemiluminescent Biosensor Based on Dual-Probe Hybridization

Sensors, 2022
To satisfy the need to develop highly sensitive methods for detecting the severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) and further enhance detection efficiency and capability, a new method was created for detecting SARS-CoV-2 of the ...
Chunying Jiang, Xihui Mu, Shuai Liu, Zhiwei Liu, Bin Du, Jiang Wang, Jianjie Xu   +6 more
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Chemical Architecture and Applications of Nucleic Acid Derivatives Containing 1,2,3-Triazole Functionalities Synthesized via Click Chemistry

Molecules, 2012
There is considerable attention directed at chemically modifying nucleic acids with robust functional groups in order to alter their properties. Since the breakthrough of copper-assisted azide-alkyne cycloadditions (CuAAC), there have been several ...
Wei Gong, Tim Efthymiou, Jean-Paul Desaulniers   +2 more
doaj   +1 more source

Electropermeabilization-based fluorescence in situ hybridization of whole-mount plant parasitic nematode specimens

MethodsX, 2019
A fluorescence in situ hybridization (FISH) protocol was developed for nematodes in which nucleic acid probes are introduced within the organism via electroporation.
Casey L. Ruark-Seward, Eric L. Davis, Tim L. Sit   +2 more
doaj   +1 more source

A Widely Applicable Urea-based Fluorescent/Colorimetric mRNA in situ Hybridization Protocol

Bio-Protocol, 2019
In situ hybridization methods are routinely employed to detect nucleic acid sequences, allowing to localize gene expression or to study chromosomal organization in their native context. These methods rely on the pairwise binding of a labeled probe to the
Chiara Sinigaglia
doaj   +1 more source