Results 21 to 30 of about 2,865,359 (294)

Highly efficient and robust π-FISH rainbow for multiplexed in situ detection of diverse biomolecules

Nature Communications, 2023
Fluorescence in situ hybridization (FISH) methods with high sensitivity are needed. Here the authors develop multiplex πFISH rainbow to detect a range of biomolecules; they also combine this with the hybridization chain reaction to develop πFISH ...
Yingfeng Tao, Xiaoliu Zhou, Leqiang Sun, Da Lin, Huaiyuan Cai, Xi Chen, Wei Zhou, Bing Yang, Zhe Hu, Jing Yu, Jing Zhang, Xiaoqing Yang, Fang Yang, Bang Shen, Wenbao Qi, Zhenfang Fu, Jinxia Dai, Gang Cao   +17 more
doaj   +1 more source

Protein-Based Anchoring Methods for Nucleic Acid Detection in Lateral Flow Format Assays

Micromachines, 2023
The use of lateral flow assays to detect nucleic acid targets has many applications including point-of-care diagnostics, environmental monitoring, and food safety.
Kira Hallerbach, Khadijeh Khederlou, Lael Wentland, Lana Senten, Steven Brentano, Brian Keefe, Elain Fu   +6 more
doaj   +1 more source

Nucleic acid nanoassembly-enhanced RNA therapeutics and diagnosis

Acta Pharmaceutica Sinica B, 2023
RNAs are involved in the crucial processes of disease progression and have emerged as powerful therapeutic targets and diagnostic biomarkers. However, efficient delivery of therapeutic RNA to the targeted location and precise detection of RNA markers ...
Mengnan Zhao, Rujing Wang, Kunmeng Yang, Yuhong Jiang, Yachen Peng, Yuke Li, Zhen Zhang, Jianxun Ding, Sanjun Shi   +8 more
doaj   +1 more source

Algorithms for Nucleic Acid Sequence Design [PDF]

, 2010
Motivated by a growing field of research focused on programming function into biomolecules, we seek to decrease the cost of high-quality rational nucleic acid sequence design while increasing its versatility and availability.
Zadeh, Joseph N.
core   +1 more source

Optimizing the specificity of nucleic acid hybridization. [PDF]

Nat Chem, 2012
The specific hybridization of complementary sequences is an essential property of nucleic acids, enabling diverse biological and biotechnological reactions and functions. However, the specificity of nucleic acid hybridization is compromised for long strands, except near the melting temperature. Here, we analytically derived the thermodynamic properties
Zhang DY, Chen SX, Yin P.
europepmc   +4 more sources

Microfluidic Chip for the Electrochemical Detection of MicroRNAs: Methylene Blue Increasing the Specificity of the Biosensor

Frontiers in Chemistry, 2022
MicroRNAs (miRNAs) are biomarkers involved in biological processes that are released by cells and found in biological fluids such as blood. The development of nucleic acid-based biosensors has significantly increased in the past 10 years because the ...
Claire Poujouly, Jérémy Le Gall, Martina Freisa, Djamila Kechkeche, David Bouville, Jihed Khemir, Pedro Gonzalez-Losada, Jean Gamby   +7 more
doaj   +1 more source

Physico-chemical foundations underpinning microarray and next-generation sequencing experiments [PDF]

, 2013
Hybridization of nucleic acids on solid surfaces is a key process involved in high-throughput technologies such as microarrays and, in some cases, next-generation sequencing (NGS).
Carlon, E, Buhot, A, Hooyberghs, J, Harrison, A., Gamble, LJ, Pozhitkov, A., Pettitt, B., Tautz, D. ; https://orcid.org/, Levicky, Rastislav, Harrison, A, Buhot, A., Carlon, E., Pozhitkov, Alexander E, Gibas, C, Halperin, A, Binder, H, Pettitt, BM, Gamble, L., Noble, P., Hooyberghs, Jef, Harrison, Andrew B., Levicky, R, Pettitt, B. Montgomery, Pozhitkov, AE, Noble, PA, Carlon, Enrico, Halperin, Avraham, Kreil, David P, Levicky, R., Ott, A, Kreil, DP, Tautz, Diethard, Binder, H., Halperin, A., Burden, Conrad John, Gibas, Cynthia, Burden, CJ, Burden, Conrad J, Kreil, David, Hooyberghs, J., Buhot, Arnaud, Gamble, Lara J., Noble, Peter A, Harrison, Andrew, Gamble, Lara J, Tautz, D, Pettitt, B Montgomery, Kreil, D., Ott, A., Binder, Hans, Burden, C., Ott, Albrecht, Pozhitkov, Alexander E., Gibas, C., Noble, Peter A.   +54 more
core   +1 more source

Stochastic Simulation of the Kinetics of Multiple Interacting Nucleic Acid Strands [PDF]

, 2013
DNA nanotechnology is an emerging field which utilizes the unique structural properties of nucleic acids in order to build nanoscale devices, such as logic gates, motors, walkers, and algorithmic structures. Predicting the structure and interactions of a
Schaeffer, Joseph Malcolm
core   +1 more source

Nucleic Acid Hybridization [PDF]

, 2020
Abbreviations -- Preface -- 1. Introduction to nucleic acid hybridization -- Structure of nucleic acids -- Forces stabilizing DNA structure -- Mismatched base pairs -- Stability of nucleic acids -- Effects of pH -- Effects of temperature -- Renaturation and hybridization -- 2.
openaire   +3 more sources

A Study of the Detection of SARS-CoV-2 ORF1ab Gene by the Use of Electrochemiluminescent Biosensor Based on Dual-Probe Hybridization

Sensors, 2022
To satisfy the need to develop highly sensitive methods for detecting the severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) and further enhance detection efficiency and capability, a new method was created for detecting SARS-CoV-2 of the ...
Chunying Jiang, Xihui Mu, Shuai Liu, Zhiwei Liu, Bin Du, Jiang Wang, Jianjie Xu   +6 more
doaj   +1 more source