Primer recognition by avian myeloblastosis virus RNA-directed DNA polymerase [PDF]
Tryptophanyl-tRNA was specifically labeled at the 3' end with [3H]tryptophan and cleaved in half with RNase under denaturing conditions, and the 3' half was shown to hybridize exclusively at the 5' end of avian myeloblastosis virus RNA. The RNA-dependent DNA polymerase of avian myeloblastosis virus is capable of efficiently binding the 3' half of the ...
R D, Brown, R W, Armentrout
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New link between the RNA polymerase II-CTD and replication stress
Conflicts between transcription and replication are a major source of replication stress. Our recent findings show that proper dephosphorylation of Serine 5 in the carboxy-terminal domain (CTD) of DNA-directed RNA polymerase II subunit RPB1 is needed to ...
Helga B. Landsverk +3 more
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Endogenous RNA-Directed DNA Polymerase Activity in Uninfected Chicken Embryos [PDF]
Early chicken embryos that are either positive or negative for group-specific antigens of avian leukosis viruses contained endogenous RNA-directed DNA polymerase activity. This endogenous DNA polymerase activity was not increased after mixture of soluble DNA polymerases isolated from chicken embryos with disrupted chicken embryo cells.
C Y, Kang, H M, Temin
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Production and processing of siRNA precursor transcripts from the highly repetitive maize genome. [PDF]
Mutations affecting the maintenance of heritable epigenetic states in maize identify multiple RNA-directed DNA methylation (RdDM) factors including RMR1, a novel member of a plant-specific clade of Snf2-related proteins.
Christopher J Hale +3 more
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DNA polymerase of reticuloendotheliosis virus: Inability to detect endogenous RNA-directed DNA synthesis [PDF]
Abstract Purified preparations of reticuloendotheliosis virus (REV) appear to lack the endogenous RNase-sensitive DNA polymerase activity present in most, if not all, avian RNA tumor viruses. Although no endogenous RNA-directed DNA polymerase could be detected in REV, we have been able to demonstrate the presence of a virion-associated DNA polymerase
Kieras, Rita M., Faras, Anthony J.
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The ability to form homodimers is essential for RDM1 to function in RNA-directed DNA methylation. [PDF]
RDM1 (RNA-DIRECTED DNA METHYLATION1) is a small plant-specific protein required for RNA-directed DNA methylation (RdDM). RDM1 interacts with RNA polymerase II (Pol II), ARGONAUTE4 (AGO4), and the de novo DNA methyltransferase DOMAINS REARRANGED ...
Taku Sasaki +4 more
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Arabidopsis C-terminal domain phosphatase-like 1 functions in miRNA accumulation and DNA methylation. [PDF]
Arabidopsis CTD-PHOSPHATASE-LIKE 1 (CPL1) is a protein phosphatase that can dephosphorylate RNA polymerase II C-terminal domain (CTD). Unlike typical CTD-phosphatases, CPL1 contains a double-stranded (ds) RNA-binding motif (dsRBM) and has been implicated
In Sil Jeong +7 more
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Inhibition of RNA-directed DNA polymerase by aurintricarboxylic acid.
Commercial-grade aurintricarboxylic acid (ATA) inhibits poly(A), poly(C) and viral RNA-directed DNA synthesis by detergent-disrupted virions of Moloney murine leukemia virus. Paper chromatography of crude ATA yields two active components, which appear to behave identically, and at least two inactive components.
J F, Givens, K F, Manly
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RNA-directed DNA polymerase from particles released by normal goose cells [PDF]
Cells from a goose embryo were shown to release particle-associated RNA-directed DNA polymerase and RNase H activities that required the presence of Nonidet P-40 for detection. The particles were not infectious and did not have endogenous DNA synthesis.
Bauer, Georg, Temin, Howard Martin
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Heterochromatic siRNAs and DDM1 independently silence aberrant 5S rDNA transcripts in Arabidopsis. [PDF]
5S ribosomal RNA gene repeats are arranged in heterochromatic arrays (5S rDNA) situated near the centromeres of Arabidopsis chromosomes. The chromatin remodeling factor DDM1 is known to maintain 5S rDNA methylation patterns while silencing transcription ...
Todd Blevins +3 more
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