Results 1 to 10 of about 2,174,576 (296)

Diverse Systems for Efficient Sequence Insertion and Replacement in Precise Plant Genome Editing

BioDesign Research, 2020
CRISPR-mediated genome editing has been widely applied in plants to make uncomplicated genomic modifications including gene knockout and base changes.
Yingxiao Zhang, Yiping Qi
doaj   +1 more source

Genetically Engineered Organization: Protein Template, Biological Recognition Sites, and Nanoparticles

Advanced Materials Interfaces, 2016
Nanoparticles and their assemblies exhibit properties that can be used for a wide range of applications. However, creating multifunctional assemblies has remained challenging. Inspired by magnetotactic bacteria, genetically engineered single building blocks from magnetosome chains are used and complemented by additional components to form fluorescent ...
Jehle, F., Valverde-Tercedor, C., Reichel, V., Carillo, M., Bennet, M., Günther, E., Wirth, R., Mickoleit, F., Zarivach, R., Schüler, D., Blank, K., Faivre, D.   +11 more
openaire   +4 more sources

Efficient Gene-Editing in Human Pluripotent Stem Cells Through Simplified Assembly of Adeno-Associated Viral (AAV) Donor Templates

Bio-Protocol
Gene-edited human pluripotent stem cells provide attractive model systems to functionally interrogate the role of specific genetic variants in relevant cell types.
Berta de La Cruz, Sanhita Mitra, Bingqing He, Melis Çelik, Debora Kaminski, Erik Smedler, Fredrik Sterky   +6 more
doaj   +1 more source

Piecemeal Buildup of the Genetic Code, Ribosomes, and Genomes from Primordial tRNA Building Blocks

Life, 2016
The origin of biomolecular machinery likely centered around an ancient and central molecule capable of interacting with emergent macromolecular complexity. tRNA is the oldest and most central nucleic acid molecule of the cell.
Derek Caetano-Anollés, Gustavo Caetano-Anollés   +1 more
doaj   +1 more source

Correction of the Allan-Herndon-Dudley syndrome-causing SLC16A2 mutation G401R in a patient derived hiPSC line

Stem Cell Research
The X-linked Allan-Herndon-Dudley syndrome (AHDS) is a genetic disorder characterized by severe psychomotor impairment, resulting from mutations in the SLC16A2 gene, which encodes the thyroid hormone transporter MCT8 (monocarboxylate transporter 8 ...
Katarzyna A. Ludwik, Robert Opitz, Sabine Jyrch, Matthias Megges, Peter Kühnen, Harald Stachelscheid   +5 more
doaj   +1 more source

EvoAVX: Island-Based Genetic Algorithm Library With AVX-512 and MPI

IEEE Access
Genetic algorithms are practical tools for solving complex optimization problems; however, the performance of many existing solutions is often limited by their inability to utilize modern CPU capabilities fully.
Adrian Kulawik, Filip Kruzel
doaj   +1 more source

Assessing Plant Genetic Diversity by Molecular Tools

Diversity, 2009
This paper is an overview of the diverse, predominantly molecular techniques, used in assessing plant genetic diversity. In recent years, there has been a significant increase in the application of molecular genetic methods for assessing the conservation
Linda Mondini, Arshiya Noorani, Mario A. Pagnotta   +2 more
doaj   +1 more source

Simply cut out – Combining CRISPR/Cas9 RNPs and transiently selected telomere vectors for marker free-gene deletion in Trichoderma atroviride

Frontiers in Genome Editing
Trichoderma atroviride is a well-known mycoparasitic fungus widely used for the biological control of fungal plant pathogens. However, genetic manipulation in this organism remains challenging due to the limited availability of versatile and efficient ...
Mario Gründlinger, Chiara Ellensohn, Leo Drechsel, Ulrike Schreiner, Siebe Pierson, Clara Baldin, Susanne Zeilinger   +6 more
doaj   +1 more source

The RNA-DNA world and the emergence of DNA-encoded heritable traits

RNA Biology
The RNA world hypothesis confers a central role to RNA molecules in information encoding and catalysis. Even though evidence in support of this hypothesis has accumulated from both experiments and computational modelling, the transition from an RNA world
Suvam Roy, Supratim Sengupta
doaj   +1 more source

Simple and Effective Squash-PCR for Rapid Genotyping of Industrial Microalgae

Life
Microalgae are recognized for their versatility in providing renewable energy, biopharmaceuticals, and nutraceuticals, attributed to their sustainable, renewable, and cost-effective nature.
Guoliang Yuan, Song Gao, Jeffrey J. Czajka, Ziyu Dai, Kyle R. Pomraning, Rylan D. Duong, Beth A. Hofstad, Shuang Deng   +7 more
doaj   +1 more source