Results 11 to 20 of about 218,953 (302)

An unbiased template of the Drosophila brain and ventral nerve cord.

PLoS ONE, 2020
The fruit fly Drosophila melanogaster is an important model organism for neuroscience with a wide array of genetic tools that enable the mapping of individual neurons and neural subtypes.
John A Bogovic, Hideo Otsuna, Larissa Heinrich, Masayoshi Ito, Jennifer Jeter, Geoffrey Meissner, Aljoscha Nern, Jennifer Colonell, Oz Malkesman, Kei Ito, Stephan Saalfeld   +10 more
doaj   +1 more source

Timed inhibition of CDC7 increases CRISPR-Cas9 mediated templated repair. [PDF]

, 2020
Repair of double strand DNA breaks (DSBs) can result in gene disruption or gene modification via homology directed repair (HDR) from donor DNA. Altering cellular responses to DSBs may rebalance editing outcomes towards HDR and away from other repair ...
A Ferreira, A Montagnoli, A Orthwein, AC Komor, B Roberts, B Verdoodt, C Anders, C Yu, CD Richardson, CD Richardson, D Szklarczyk, DW Huang, E Lingeman, ES Koltun, FK Zimmermann, H Ghezraoui, J Song, JA Doudna, JE Haber, JP Duxin, K Jayathilaka, K Nakanishi, K Okita, KJ Livak, L Lei, L Pessoa-Brandão, LA Gilbert, LN Brandão, M Cavazzana, M Lei, M Yamada, MA DeWitt, MD Canny, MD Rainey, MH Porteus, N Hustedt, N Zielke, NK Sasi, NR Pannunzio, R Ceccaldi, RC Alver, S Hughes, S Lin, SB Thyme, T Maruyama, T Takenaka, T Tsuji, TA Day, TL Roth, TS Nambiar, U Eichenlaub-Ritter, VT Chu, W Li, X Liang, Z Romero   +54 more
core   +1 more source

Biasing AlphaFold2 to predict GPCRs and kinases with user-defined functional or structural properties

Frontiers in Molecular Biosciences, 2023
Determining the three-dimensional structure of proteins in their native functional states has been a longstanding challenge in structural biology. While integrative structural biology has been the most effective way to get a high-accuracy structure of ...
Davide Sala, Peter W. Hildebrand, Jens Meiler, Jens Meiler, Jens Meiler   +4 more
doaj   +1 more source

Nonenzymatic RNA-templated Synthesis of N3′→P5′ Phosphoramidate DNA

Bio-Protocol, 2020
The RNA world hypothesis describes a scenario where early life forms relied on RNA to govern both inheritance and catalyze useful chemical reactions.
Derek O'Flaherty, Lijun Zhou, Jack Szostak   +2 more
doaj   +1 more source

Proof of concept for multiplex amplicon sequencing for mutation identification using the MinION nanopore sequencer

Scientific Reports, 2022
Rapid, cost-effective identification of genetic variants in small candidate genomic regions remains a challenge, particularly for less well equipped or lower throughput laboratories.
Whitney Whitford, Victoria Hawkins, Kriebashne S. Moodley, Matthew J. Grant, Klaus Lehnert, Russell G. Snell, Jessie C. Jacobsen   +6 more
doaj   +1 more source

Oncogene inference optimization using constraint-based modelling incorporated with protein expression in normal and tumour tissues [PDF]

Royal Society Open Science, 2020
Cancer cells are known to exhibit unusual metabolic activity, and yet few metabolic cancer driver genes are known. Genetic alterations and epigenetic modifications of cancer cells result in the abnormal regulation of cellular metabolic pathways that are ...
Wu-Hsiung Wu, Fan-Yu Li, Yi-Chen Shu, Jin-Mei Lai, Peter Mu-Hsin Chang, Chi-Ying F. Huang, Feng-Sheng Wang   +6 more
doaj   +1 more source

Bionanomaterials from plant viruses [PDF]

, 2015
Plant virus capsids have emerged as useful biotemplates for material synthesis. All plant virus capsids are assembled with high-precision, three-dimensional structures providing nanoscale architectures that are highly monodisperse, can be produced in ...
Aljabali, Alaa A. A., Evans, David
core   +2 more sources

Design, modeling and synthesis of an in vitro transcription rate regulatory circuit [PDF]

, 2008
This paper describes the design, modeling and realization of a synthetic in vitro circuit that aims at regulating the rate of mRNA transcription. Two DNA templates are designed to interact through their transcripts, creating negative feedback loops that ...
Forsberg, Per-Ola, Franco, Elisa, Murray, Richard M.   +2 more
core   +2 more sources

Transformation and CRISPR-Cas9-mediated homologous recombination in the fungus Rhizopus microsporus

STAR Protocols, 2022
Summary: Here, we describe a reliable approach for targeted DNA integrations in the genome of R. microsporus, one of the main causal agents of mucormycosis.
Carlos Lax, María Isabel Navarro-Mendoza, Carlos Pérez-Arques, Eusebio Navarro, Francisco Esteban Nicolás, Victoriano Garre   +5 more
doaj   +1 more source

Selfishness versus functional cooperation in a stochastic protocell model [PDF]

, 2010
How to design an "evolvable" artificial system capable to increase in complexity? Although Darwin's theory of evolution by natural selection obviously offers a firm foundation, little hope of success seems to be expected from the explanatory adequacy of ...
Agresti, Beeby, Bell, Biebricher, Branciamore, Chen, Crow, Deamer, Drake, Elias Zintzaras, Ewens, Eörs Szathmáry, Fontanari, Grey, Gánti, Gánti, Haldane, Kacser, Kacser, Koch, Kondo, Kun, Könnyű, Luisi, Luisi, Luisi, Mansy, MathWorks, Mauro Santos, Maynard Smith, Mills, Moran, Niesert, Niesert, Oberholzer, Pohorille, Reader, Santos, Schaffner, Scheuring, Silvestre, Spiegelman, Stadler, Szabó, Szathmáry, Szathmáry, Szathmáry, Szathmáry, Szathmáry, Szostak, Takeuchi, Takeuchi, Traulsen, von Kiedrowski, von Kiedrowski, Whitesides, Zintzaras   +56 more
core   +2 more sources