Results 41 to 50 of about 119,454 (267)

Multiple Antibiotic Resistance Plasmids Allow Scalable, PCR-Mediated DNA Manipulation and Near-Zero Background Cloning

Food Technology and Biotechnology, 2016
We have constructed two plasmids that can be used for cloning as templates for PCR-based gene disruption, mutagenesis and the construction of DNA chromosome translocation cassettes.
Remigiusz Arnak, Burcin Altun, Valentina Tosato, Carlo V. Bruschi   +3 more
doaj   +1 more source

Molecular Profile of Cogongrass [Imperata cylindrica (L.) Raeusch] in Java Island Based on trnT(UGU)-trnL(UAA) IGS Sequences

Jurnal Biodjati, 2023
Cogongrass is one of the top ten problem-causing weeds in the world that is invasively spread over many tropical and subtropical regions, including Java Island, the Republic of Indonesia. The wide distribution is possibly related to their adaptability to
Murni Dwiati, Salma Auliya Rahmah, Agus Hery Susanto   +2 more
doaj   +1 more source

Drug Resistance in Cancers: A Free Pass for Bullying

Cells, 2022
The cancer burden continues to grow globally, and drug resistance remains a substantial challenge in cancer therapy. It is well established that cancerous cells with clonal dysplasia generate the same carcinogenic lesions.
Jing Li, Xiao Li, Qie Guo
doaj   +1 more source

Universal Multiplex PCR: a novel method of simultaneous amplification of multiple DNA fragments

Plant Methods, 2012
Background Multiplex PCR has been successfully applied in many areas since it was first reported in 1988; however, it suffers from poor universality.
Wen Daxing, Zhang Chunqing
doaj   +1 more source

ngsJulia: population genetic analysis of next-generation DNA sequencing data with Julia language [version 3; peer review: 1 approved, 2 approved with reservations]

F1000Research, 2023
A sound analysis of DNA sequencing data is important to extract meaningful information and infer quantities of interest. Sequencing and mapping errors coupled with low and variable coverage hamper the identification of genotypes and variants and the ...
Alex Mas-Sandoval, Chenyu Jin, Marco Fracassetti, Matteo Fumagalli   +3 more
doaj   +1 more source

Transferrin receptor 1‐mediated iron uptake supports thermogenic activation in human cervical‐derived adipocytes

FEBS Letters, EarlyView.
In this study, we found that human cervical‐derived adipocytes maintain intracellular iron level by regulating the expression of iron transport‐related proteins during adrenergic stimulation. Melanotransferrin is predicted to interact with transferrin receptor 1 based on in silico analysis.
Rahaf Alrifai, Mizuki Seo, Gyath Karadsheh, Fachrur Rizal Mahendra, Máté Á Demény, Ferenc Győry, János András Mótyán, László Fésüs, Endre Kristóf, Rini Arianti   +9 more
wiley   +1 more source

Genetic diversity and differentiation of Pinus sylvestris L. from the IUFRO 1982 provenance trial revealed by AFLP analysis [PDF]

Archives of Biological Sciences, 2015
DNA markers have become effective tools in genetic diversity studies of forest trees. However, molecular marker analyses are associated with laborious and costly effort. One of the possibilities to overcome these constraints is to analyze bulked
Androsiuk Piotr, Ciaglo-Androsiuk Sylwia, Urbaniak Lech   +2 more
doaj   +1 more source

The planar cell polarity protein Vangl2 interacts with the PDZ‐domains of Scribble but not with a unique PDZ‐like domain in Inturned

FEBS Letters, EarlyView.
Structural and biochemical characterisations show that the planar cell polarity (PCP) protein Inturned harbours a unique PDZ‐like domain that does not bind canonical PDZ‐binding motifs (PBMs) like that of another PCP protein Vangl2. In contrast, the apical‐basal polarity protein Scribble contains four PDZ domains that bind Vangl2, but one PDZ domain ...
Stephan Wilmes, Jan Brysch, Carmen Gelze, Lilli Meier, Daniel Kümmel   +4 more
wiley   +1 more source

Genetic control of morphological transitions in a coacervating protein template

Soft Matter
Point mutations and thermal energy enable control over the assembly of a thermoresponsive silica-binding elastin-like polypeptide, while MD simulations and silicification reveal how the soft template transitions from micelle to proteinosome.
William C. Wixson, Nada Y. Naser, Aditya Sonpal, Jim Pfaendtner, François Baneyx   +4 more
openaire   +2 more sources

Tau acetylation at K331 has limited impact on tau pathology in vivo

FEBS Letters, EarlyView.
We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto, Yukio Matsuba, Mika Takahashi, Takaomi C. Saido   +3 more
wiley   +1 more source