Results 31 to 40 of about 218,953 (302)
Use of genetic markers for the detection of off-types for DUS phenotypic traits in the inbreeding crop, barley [PDF]
, 2020 Detection of crop off-types is of interest for multiple uses, including the assessment of uniformity for new plant variety applications during distinctness, uniformity and stability (DUS) testing for the awarding of plant breeders’ rights (PBR). Here,
Cockram, James +4 more
core +1 more source
Probing Ambiguous Base‐Pairs by Genetic Transformation with XNA Templates
ChemBioChem, 2014 AbstractThe templating potential of anhydrohexitol oligonucleotides bearing ambiguous bases was studied in vivo, by using a selection screen for mosaic heteroduplex plasmids in Escherichia coli. 1,5‐Anhydro‐2,3‐dideoxy‐2‐(5‐nitroindazol‐1‐yl)‐D‐arabino‐hexitol showed the greatest ambiguity among the three nucleosides tested.
Pezo, Valérie +4 more
openaire +3 more sources
Journal of Nucleic Acids, 2012 For the site-specific labeling and modification of RNA by genetic alphabet expansion, we developed a PCR and transcription system using two hydrophobic unnatural base pairs: 7-(2-thienyl)-imidazo[4,5-b]pyridine (Ds) and 2-nitro-4-propynylpyrrole (Px) as ...
Michiko Kimoto +3 more
doaj +1 more source
G3: Genes, Genomes, Genetics, 2019 DNA double-strand breaks (DSBs) are especially toxic DNA lesions that, if left unrepaired, can lead to wide-ranging genomic instability. Of the pathways available to repair DSBs, the most accurate is homologous recombination (HR), where a homologous ...
Joel Fernandez +3 more
doaj +1 more source
Assessing DNA Sequence Alignment Methods for Characterizing Ancient Genomes and Methylomes
Frontiers in Ecology and Evolution, 2020 Applying high-throughput DNA sequencing technologies to the ancient DNA molecules preserved in subfossil material can provide genetic information from past individuals, populations, and communities at the genomic scale.
Marine Poullet +2 more
doaj +1 more source
Food Technology and Biotechnology, 2016 We have constructed two plasmids that can be used for cloning as templates for PCR-based gene disruption, mutagenesis and the construction of DNA chromosome translocation cassettes.
Remigiusz Arnak +3 more
doaj +1 more source
A Survey on Joint Object Detection and Pose Estimation using Monocular Vision [PDF]
, 2018 In this survey we present a complete landscape of joint object detection and pose estimation methods that use monocular vision. Descriptions of traditional approaches that involve descriptors or models and various estimation methods have been provided ...
Patil, Aniruddha V, Rabha, Pankaj
core +2 more sources
Emerging PCR-based techniques to study HIV-1 reservoir persistence [PDF]
, 2020 While current antiretroviral therapies are able to halt HIV-1 progression, they are not curative, as an interruption of treatment usually leads to viral rebound.
Cole, Basiel +4 more
core +2 more sources
Jurnal Biodjati, 2023 Cogongrass is one of the top ten problem-causing weeds in the world that is invasively spread over many tropical and subtropical regions, including Java Island, the Republic of Indonesia. The wide distribution is possibly related to their adaptability to
Murni Dwiati +2 more
doaj +1 more source
Cas9 interrogates DNA in discrete steps modulated by mismatches and supercoiling. [PDF]
, 2020 The CRISPR-Cas9 nuclease has been widely repurposed as a molecular and cell biology tool for its ability to programmably target and cleave DNA. Cas9 recognizes its target site by unwinding the DNA double helix and hybridizing a 20-nucleotide section of ...
Aris, Kevin D Palacio +5 more
core +3 more sources