Results 41 to 50 of about 2,512,760 (207)

An optimized protocol for stepwise optimization of real-time RT-PCR analysis

Horticulture Research, 2021
Computational tool-assisted primer design for real-time reverse transcription (RT) PCR (qPCR) analysis largely ignores the sequence similarities between sequences of homologous genes in a plant genome.
Fangzhou Zhao, N. Maren, Pawel Z. Kosentka, Yingxia Liao, Hongyan Lu, James R. Duduit, De-Bao Huang, H. Ashrafi, Tuan-Jie Zhao, A. Huerta, T. Ranney, Wu-Sheng Liu   +11 more
semanticscholar   +1 more source

Direct In Situ RT-PCR [PDF]

, 2011
In situ polymerase chain reaction (PCR) is a histological technique that exploits the advantages of PCR for detection of mRNA directly in tissue sections. It somehow conjugates together PCR and in situ hybridization that is more traditionally employed for mRNA localization in cell organelles, intact cells, or tissue sections. This chapter describes the
LOSSI, Laura, GAMBINO, Graziana, SALIO, Chiara, MERIGHI, Adalberto   +3 more
openaire   +3 more sources

Detecção de ácidos nucléicos de Brucella spp., Leptospira spp., herpesvirus bovino e vírus da diarréia viral bovina, em fetos bovinos abortados e em animais mortos no perinatal Detection of Brucella spp., Leptospira spp., bovine herpesvirus and bovine viral diarrhea virus nucleic acids in aborted fetuses and bovines dead perinatal

Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 2006
Samples of 114 bovine fetuses and 10 calves, which dead in perinatal period, were examined for detection of DNA. The most common detected agent was Brucella spp. in 17 samples (13.7%) followed by Leptospira spp. in 4 cases (3.2%),bovine herpesvirus (BHV)
A. Cortez, A.M.G. Castro, M.B. Heinemann, R.M. Soares, R.C. Leite, E. Scarcelli, M.E. Genovez, A.A. Alfieri, L.J. Richtzenhain   +8 more
doaj   +1 more source

Insight into the practical performance of RT-PCR testing for SARS-CoV-2 using serological data: a cohort study

The Lancet Microbe, 2021
Summary Background Virological detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) through RT-PCR has limitations for surveillance. Serological tests can be an important complementary approach.
Zhen Zhang, Q. Bi, S. Fang, Lan Wei, Xin Wang, Jian-Fan He, Yong-Sheng Wu, Xiaojian Liu, Wei Gao, Ren-Li Zhang, W. Gong, Q. Su, A. Azman, J. Lessler, X. Zou   +14 more
semanticscholar   +1 more source

Performance of an alternative RT-PCR procedure using residual sample from the Panbio™ Ag COVID-19 test

Brazilian Journal of Infectious Diseases, 2021
Introduction: In the current standard of care (SoC) RT-PCR method for COVID-19, the patient's swab was extracted in viral transport media (VTM). For the Panbio™ COVID-19 Ag Rapid Test, the patient swab is flushed out in extraction buffer, of which a ...
Terezinha Marta Pereira Pinto Castineiras, Érica Ramos dos Santos Nascimento, Débora Souza Faffe, Rafael Mello Galliez, Diana Mariani, Isabela de Carvalho Leitão, Mayla Gabryele Miranda de Melo, Orlando Costa Ferreira, Amilcar Tanuri   +8 more
doaj   +1 more source

Detection of Novel Coronavirus by RT-PCR in Stool Specimen from Asymptomatic Child, China

Emerging Infectious Diseases, 2020
We report an asymptomatic child who was positive for a coronavirus by reverse transcription PCR in a stool specimen 17 days after the last virus exposure. The child was virus positive in stool specimens for at least an additional 9 days.
A. Tang, Zhen-Dong Tong, Hong-Ling Wang, Ya-Xin Dai, Ke-Feng Li, Jie-Nan Liu, Wen-Jie Wu, Chen Yuan, Meng-lu Yu, Peng Li, Jian-Bo Yan   +10 more
semanticscholar   +1 more source

BREAST CANCER TYPING USING RT-PCR ASSAY

Сибирский онкологический журнал, 2019
Introduction. Adjuvant systemic therapy remains one of the main options for treating breast cancer. Results of standard immunohistochemical studies are not always a criterion for selecting systemic therapy.
V. K. Bozhenko, I. D. Trotsenko, E. A. Kudinova, S. G. Vardanyan, M. V. Zakharenko, V. A. Solodky, M. V. Makarova   +6 more
doaj   +1 more source

Optimisation of COVID‐19 diagnostic pathways in acute hospital admissions to prevent nosocomial transmission

The Clinical Respiratory Journal, 2022
Introduction In the management of acute hospital admissions during the COVID‐19 pandemic, safe patient cohorting depends on robust admission diagnostic strategies.
Robert Livingstone, Alexander Woodhead, Megha Bhandari, James Dias, Trevor Smith, Tom Havelock, Matthew Stammers   +6 more
doaj   +1 more source

Rapid SARS-CoV-2 antigen detection assay in comparison with real-time RT-PCR assay for laboratory diagnosis of COVID-19 in Thailand

Virology Journal, 2020
Background The Coronavirus disease 2019 (COVID-19) pandemic continues to spread across the world. Hence, there is an urgent need for rapid, simple, and accurate tests to diagnose severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection ...
Chutikarn Chaimayo, Bualan Kaewnaphan, Nattaya Tanlieng, N. Athipanyasilp, Rujipas Sirijatuphat, M. Chayakulkeeree, Nasikarn Angkasekwinai, R. Sutthent, Nattawut Puangpunngam, Theerawoot Tharmviboonsri, O. Pongraweewan, S. Chuthapisith, Y. Sirivatanauksorn, W. Kantakamalakul, N. Horthongkham   +14 more
semanticscholar   +1 more source

RT-PCR Protocols - Methods in Molecular Biology

European Journal of Histochemistry, 2011
“The first record I have of it, is when I made a computer file which I usually did whenever I had an idea, that would have been on the Monday when I got back, and I called it Chain Reaction.POL, meaning polymerase.
Manuela Monti
doaj   +1 more source