Results 21 to 30 of about 2,512,760 (207)

Duration of infectiousness and correlation with RT-PCR cycle threshold values in cases of COVID-19, England, January to May 2020

Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin, 2020
Severe acute respiratory syndrome coronavirus 2 viral load in the upper respiratory tract peaks around symptom onset and infectious virus persists for 10 days in mild-to-moderate coronavirus disease (n = 324 samples analysed). RT-PCR cycle threshold (Ct)
A. Singanayagam, M. Patel, A. Charlett, James A. Lopez Bernal, V. Saliba, J. Ellis, S. Ladhani, M. Zambon, R. Gopal   +8 more
semanticscholar   +1 more source

Reproducibility in the Quantification of mRNA Levels by RT-PCR-ELISA and RT Competitive-PCR-ELISA

BioTechniques, 1998
The use of reverse transcription (RT) PCR for relative quantitation of gene transcripts relies on the reproducibility of the individual RT, PCR and product measurement steps.
L.L. Hall, G.R. Bicknell, L. Primrose, J.H. Pringle, J.A. Shaw, P.N. Furness   +5 more
doaj   +1 more source

False-negative results of initial RT-PCR assays for COVID-19: A systematic review

medRxiv, 2020
Background: Cases with negative reverse transcription-polymerase chain reaction (RT-PCR) results at initial testing for suspicion of SARS-CoV-2 infection, and found to be positive in a subsequent test, are considered as RT-PCR false-negative cases. False-
I. Arevalo-Rodriguez, Diana Buitrago-García, Daniel Simancas-Racines, Paula Zambrano-Achig, R. del Campo, A. Ciapponi, O. Sued, L. Martínez-García, A. Rutjes, N. Low, P. Bossuyt, J. Pérez-Molina, J. Zamora   +12 more
semanticscholar   +1 more source

Minimizing errors in RT-PCR detection and quantification of SARS-CoV-2 RNA for wastewater surveillance

Science of the Total Environment, 2021
Wastewater surveillance for pathogens using reverse transcription-polymerase chain reaction (RT-PCR) is an effective and resource-efficient tool for gathering community-level public health information, including the incidence of coronavirus disease-19 ...
W. Ahmed, S. Simpson, P. Bertsch, K. Bibby, A. Bivins, L. Blackall, S. Bofill-Mas, A. Bosch, J. Brandão, P. Choi, M. Ciesielski, E. Donner, Nishita Dsouza, A. Farnleitner, Daniel Gerrity, Raul A. Gonzalez, J. Griffith, P. Gyawali, C. Haas, K. Hamilton, H. Hapuarachchi, V. Harwood, Rehnuma Haque, Greg Jackson, Stuart J. Khan, W. Khan, M. Kitajima, A. Korajkic, G. La Rosa, B. Layton, E. Lipp, S. McLellan, Brian R. McMinn, G. Medema, S. Metcalfe, W. Meijer, J. Mueller, Heather Murphy, C. Naughton, Rachel Noble, Sudhi Payyappat, S. Petterson, T. Pitkänen, V. Rajal, B. Reyneke, F. Roman, J. Rose, M. Rusiñol, M. Sadowsky, Laura Sala-Comorera, Y. X. Setoh, S. Sherchan, K. Sirikanchana, Wendy J. M. Smith, Joshua Steele, R. Sabburg, E. Symonds, P. Thai, K. Thomas, J. Tynan, S. Toze, Janelle R. Thompson, A. Whiteley, J. Wong, D. Sano, S. Wuertz, Irene Xagoraraki, Qian Zhang, A. Zimmer-Faust, O. Shanks   +69 more
semanticscholar   +1 more source

Real-time quantification of microRNAs by stem–loop RT–PCR

Nucleic Acids Research, 2005
A novel microRNA (miRNA) quantification method has been developed using stem–loop RT followed by TaqMan PCR analysis. Stem–loop RT primers are better than conventional ones in terms of RT efficiency and specificity.
Cai-Fu Chen, Dana Ridzon, A. Broomer, Zhaohui Zhou, Danny H. Lee, Julie T. Nguyen, M. Barbisin, Nan-Lan Xu, Vikram R. Mahuvakar, M. R. Andersen, K. Lao, K. Livak, Karl J. Guegler   +12 more
semanticscholar   +1 more source

Stability issues of RT‐PCR testing of SARS‐CoV‐2 for hospitalized patients clinically diagnosed with COVID‐19

Journal of Medical Virology, 2020
In this study, we collected a total of 610 hospitalized patients from Wuhan between February 2, 2020, and February 17, 2020. We reported a potentially high false negative rate of real‐time reverse‐transcriptase polymerase chain reaction (RT‐PCR) testing ...
Yafang Li, L. Yao, Jia-Wei Li, Lei Chen, Yiyan Song, Z. Cai, Chun-Hua Yang   +6 more
semanticscholar   +1 more source

Comparison of Home Antigen Testing With RT-PCR and Viral Culture During the Course of SARS-CoV-2 Infection.

JAMA Internal Medicine, 2022
Importance As self-collected home antigen tests become widely available, a better understanding of their performance during the course of SARS-CoV-2 infection is needed.
Victoria T. Chu, N. Schwartz, M. Donnelly, Meagan R. Chuey, R. Soto, A. Yousaf, Emily N Schmitt-Matzen, Sadia Sleweon, Jasmine D. Ruffin, N. Thornburg, J. Harcourt, A. Tamin, Gimin Kim, Jennifer M. Folster, Laura J Hughes, S. Tong, Ginger Stringer, B. Albanese, S. Totten, Meghan M. Hudziec, Shannon R. Matzinger, Elizabeth A. Dietrich, Sarah W. Sheldon, Sarah Stous, E. McDonald, B. Austin, M. Beatty, J. E. Staples, Marie E. Killerby, Christopher H. Hsu, J. Tate, Hannah L. Kirking, Almea M. Matanock   +32 more
semanticscholar   +1 more source

Analysis of the initial lot of the CDC 2019-Novel Coronavirus (2019-nCoV) real-time RT-PCR diagnostic panel

PLoS ONE, 2021
At the start of the COVID-19 pandemic, the Centers for Disease Control and Prevention (CDC) designed, manufactured, and distributed the CDC 2019-Novel Coronavirus (2019-nCoV) Real-Time RT-PCR Diagnostic Panel for SARS-CoV-2 detection.
Justin S. Lee, J. Goldstein, Jonathan L. Moon, O. Herzegh, D. Bagarozzi, M. Oberste, H. Hughes, K. Bedi, Dorothie Gerard, Brenique Cameron, Christopher M. Benton, A. Chida, Ausaf Ahmad, David Petway, Xiao-Ling Tang, Nicky Sulaiman, Dawit Teklu, D. Batra, Dakota Howard, Mili Sheth, W. Kuhnert, S. Bialek, C. Hutson, J. Pohl, D. Carroll   +24 more
semanticscholar   +1 more source

Development of an RNA Extraction Protocol for Norovirus from Raw Oysters and Detection by qRT-PCR and Droplet-Digital RT-PCR

Foods, 2021
Foodborne viruses such as norovirus and hepatitis A virus cause frequent outbreaks associated with the consumption of raw or undercooked oysters. Viral particles are bioaccumulated in the oyster’s digestive glands, making RNA extraction and RT-PCR ...
Daniel Plante, Julio Alexander Bran Barrera, Maude Lord, Irène Iugovaz, Neda Nasheri   +4 more
doaj   +1 more source

Real-time RT-PCR in COVID-19 detection: issues affecting the results

Expert Review of Molecular Diagnostics, 2020
Due to the rapid spread and increasing number of coronavirus disease 19 (COVID-19) cases caused by a new coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), rapid and accurat...
A. Tahamtan, A. Ardebili
semanticscholar   +1 more source