Results 21 to 30 of about 1,256,609 (306)

Differential expression of cellulose synthase (CesA) gene transcripts in potato as revealed by QRT-PCR [PDF]

open access: yes, 2009
Two transgenic potato lines, csr2–1 and csr4–8 that contained two different antisense cellulose synthase (CesA) genes, csr2 and csr4, respectively were crossed.
Jacobsen, E.   +3 more
core   +2 more sources

Species-specific Real Time-PCR primers/probe systems to identify fish parasites of the genera Anisakis, Pseudoterranova and Hysterothylacium (Nematoda: Ascaridoidea) [PDF]

open access: yes, 2018
Ascaridoid nematodes belonging to the genera Anisakis and Pseudoterranova are heteroxenous parasites, involving marine mammals as definitive hosts in their life-cycles, whereas crustaceans (krill), fish and squids acting as intermediate/paratenic hosts.
Colantoni, Alessandra   +5 more
core   +1 more source

Detecção de ácidos nucléicos de Brucella spp., Leptospira spp., herpesvirus bovino e vírus da diarréia viral bovina, em fetos bovinos abortados e em animais mortos no perinatal Detection of Brucella spp., Leptospira spp., bovine herpesvirus and bovine viral diarrhea virus nucleic acids in aborted fetuses and bovines dead perinatal

open access: yesArquivo Brasileiro de Medicina Veterinária e Zootecnia, 2006
Samples of 114 bovine fetuses and 10 calves, which dead in perinatal period, were examined for detection of DNA. The most common detected agent was Brucella spp. in 17 samples (13.7%) followed by Leptospira spp. in 4 cases (3.2%),bovine herpesvirus (BHV)
A. Cortez   +8 more
doaj   +1 more source

Detection of arenavirus in a peripheral odontogenic fibromyxoma in a red tail boa (Boa constrictor constrictor) with inclusion body disease [PDF]

open access: yes, 2015
A captive bred red tail boa (Boa constrictor constrictor) was presented with a large intraoral mass originating from the buccal gingiva, attached to the right dentary teeth row.
Ducatelle, Richard   +4 more
core   +1 more source

On joint maximum-likelihood estimation of PCR efficiency and initial amount of target [PDF]

open access: yes, 2007
We consider the problem of estimating unknown parameters of the real-time polymerase chain reaction (RTPCR) from noisy observations. The joint ML estimator of the RT-PCR efficiency and the initial number of DNA target molecules is derived.
Hassibi, A., Hassibi, B., Vikalo, H.
core   +1 more source

Evaluation of the Effects of DNase Treatment on Signal Specificity in RT-PCR and In Situ RT-PCR

open access: yesBioTechniques, 1998
In this study, the effects of DNase treatment on the specificity of reverse transcription (RT)-PCR have been investigated on different samples, containing RNA, DNA, DNA/RNA and DNA/cDNA.
Karin Ivarsson, Birgitta Weijdegård
doaj   +1 more source

Performance of an alternative RT-PCR procedure using residual sample from the Panbio™ Ag COVID-19 test

open access: yesBrazilian Journal of Infectious Diseases, 2021
Introduction: In the current standard of care (SoC) RT-PCR method for COVID-19, the patient's swab was extracted in viral transport media (VTM). For the Panbio™ COVID-19 Ag Rapid Test, the patient swab is flushed out in extraction buffer, of which a ...
Terezinha Marta Pereira Pinto Castineiras   +8 more
doaj   +1 more source

Complete Genome Sequence of Diaphorina citri-associated C virus, a Novel Putative RNA Virus of the Asian Citrus Psyllid, Diaphorina citri. [PDF]

open access: yes, 2016
We present here the complete nucleotide sequence and genome organization of a novel putative RNA virus identified in field populations of the Asian citrus psyllid, Diaphorina citri, through sequencing of the transcriptome followed by reverse ...
Falk, Bryce W   +2 more
core   +1 more source

BREAST CANCER TYPING USING RT-PCR ASSAY

open access: yesСибирский онкологический журнал, 2019
Introduction. Adjuvant systemic therapy remains one of the main options for treating breast cancer. Results of standard immunohistochemical studies are not always a criterion for selecting systemic therapy.
V. K. Bozhenko   +6 more
doaj   +1 more source

Optimisation of COVID‐19 diagnostic pathways in acute hospital admissions to prevent nosocomial transmission

open access: yesThe Clinical Respiratory Journal, 2022
Introduction In the management of acute hospital admissions during the COVID‐19 pandemic, safe patient cohorting depends on robust admission diagnostic strategies.
Robert Livingstone   +6 more
doaj   +1 more source

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