Results 11 to 20 of about 2,512,760 (207)
Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR
Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin, 2020 Background The ongoing outbreak of the recently emerged novel coronavirus (2019-nCoV) poses a challenge for public health laboratories as virus isolates are unavailable while there is growing evidence that the outbreak is more widespread than initially ...
V. Corman +23 more
semanticscholar +1 more source
Sensitivity of Chest CT for COVID-19: Comparison to RT-PCR
Radiology, 2020 In a series of 51 patients with chest CT and real-time polymerase chain reaction assay (RT-PCR) performed within 3 days, the sensitivity of CT for 2019 novel coronavirus infection was 98% and that ...
Yicheng Fang +6 more
semanticscholar +1 more source
Chest CT for Typical 2019-nCoV Pneumonia: Relationship to Negative RT-PCR Testing
Radiology, 2020 Some patients with positive chest CT findings may present with negative results of real time reverse-transcription–polymerase chain- reaction (RT-PCR) for 2019 novel coronavirus (2019-nCoV). In this report, we present chest CT findings from five patients
Xingzhi Xie +5 more
semanticscholar +1 more source
European Journal of Medical and Health Sciences, 2021 Purpose: Prove that Computed Tomography (CT) is a more effective diagnostic tool for diagnosing Coronavirus Disease 2019 (COVID-19) in comparison with Reverse Transcription Polymerase Chain Reaction (RT-PCR) due to its higher sensitivity.
Mohammed Hjouj +7 more
semanticscholar +1 more source
A brief history of RT-PCR and our laboratory experience with SARS-CoV-2 analyses using RT-PCR
Jurnal Teknologi Laboratorium, 2022 Polymerase Chain Reaction (PCR) is in vitro replication that allows accelerated amplification of certain sequences in small DNA fragments. A sensitive technique, only traces of DNA are needed for PCR to produce enough copies to be analyzed.
Naci Omer Alayunt
doaj +1 more source
SARS-CoV-2 RT-PCR positivity rate and predictor factors for RT-PCR results in COVID-19 disease
The Journal of Association of Chest Physicians, 2023 Background/Aim: In December 2019, a novel coronavirus was identified and caused the SARS-CoV-2 (COVID-19) pandemic. The gold standard diagnostic method is the detection of nucleic acid in respiratory tract samples by real-time polymerase chain reaction ...
Melike Badoglu +2 more
doaj +1 more source
BioTechniques, 1996 Reverse transcription-PCR (RT-PCR) has traditionally required time-consuming RNA extraction and purification. This report demonstrates that one can completely avoid the RNA extraction step in RT-PCR by basing the comparison of samples on cell number ...
R.J. Klebe +5 more
doaj +1 more source
American journal of hematology/oncology, 2023 Reverse transcription polymerase chain reaction (RT‐PCR) for BCR::ABL1 is the most common and widely accepted method of measurable residual disease (MRD) assessment in Philadelphia chromosome‐positive acute lymphoblastic leukemia (Ph+ ALL); however, RT ...
N. Short +17 more
semanticscholar +1 more source
Genome Biology, 2002 BackgroundGene-expression analysis is increasingly important in biological research, with real-time reverse transcription PCR (RT-PCR) becoming the method of choice for high-throughput and accurate expression profiling of selected genes.
J. Vandesompele +6 more
semanticscholar +1 more source
Positive RT-PCR Test Results in Patients Recovered From COVID-19.
Journal of the American Medical Association (JAMA), 2020 This case series describes reverse transcriptase–polymerase chain reaction (RT-PCR) test results in 4 health professionals discharged from hospitalization or quarantine after 2 negative RT-PCR test results and resolution of clinical COVID-19 infection.
Lan Lan +6 more
semanticscholar +1 more source