Results 11 to 20 of about 1,682,968 (306)

Comparison of Three RT-PCR Methods [PDF]

BioTechniques, 1998
Various approaches can now be taken for amplification of RNA transcripts using the polymerase chain reaction (PCR). Here, we compare three such methods: (i) uncoupled reverse transcription (RT)-PCR (using separate reactions for cDNA synthesis and PCR ...
Loryn N. Sellner, Gavin R. Turbett
doaj   +3 more sources

RT-PCR Without RNA Isolation

BioTechniques, 1996
Reverse transcription-PCR (RT-PCR) has traditionally required time-consuming RNA extraction and purification. This report demonstrates that one can completely avoid the RNA extraction step in RT-PCR by basing the comparison of samples on cell number ...
R.J. Klebe, G.M. Grant, A.M. Grant, M.A. Garcia, T.A. Giambernardi, G.P. Taylor   +5 more
doaj   +3 more sources

Quantitative RT-PCR: Pitfalls and Potential

BioTechniques, 1999
Reverse transcription PCR (RT-PCR) represents a sensitive and powerful tool for analyzing RNA. While it has tremendous potential for quantitative applications, a comprehensive knowledge of its technical aspects is required. Successful quantitative RT-PCR
Willard M. Freeman, Stephen J. Walker, Kent E. Vrana   +2 more
doaj   +3 more sources

Exploring Demographic Properties and Amplification Efficiency of N and ORF-1ab Gene in Suspected COVID-19 Patients in Dhaka, Bangladesh

Update Dental College Journal, 2022
Background: In late December 2019, an outbreak of pneumonia with an unknown cause occurs in Wuhan, China. Later, the causative agent was identified as SARS-CoV-2.
Sumaiya khatun , Md Moazzem Hossain , Md Zaforullah Chowdhury, Mamun Mia, Nazmun Nahar Munny, Fatima Tuj Johora   +5 more
doaj   +3 more sources

RT-PCR for multi-copy genes

BioTechniques, 2013
Kristie Nybo
doaj   +2 more sources

RT-PCR

, 2022
RT-PCR of ...
Haomiao Yu (12913538)
core   +1 more source

A brief history of RT-PCR and our laboratory experience with SARS-CoV-2 analyses using RT-PCR

Jurnal Teknologi Laboratorium, 2022
Polymerase Chain Reaction (PCR) is in vitro replication that allows accelerated amplification of certain sequences in small DNA fragments. A sensitive technique, only traces of DNA are needed for PCR to produce enough copies to be analyzed.
Naci Omer Alayunt
doaj   +1 more source

SARS-CoV-2 RT-PCR positivity rate and predictor factors for RT-PCR results in COVID-19 disease

The Journal of Association of Chest Physicians, 2023
Background/Aim: In December 2019, a novel coronavirus was identified and caused the SARS-CoV-2 (COVID-19) pandemic. The gold standard diagnostic method is the detection of nucleic acid in respiratory tract samples by real-time polymerase chain reaction ...
Melike Badoglu, Sevtap Gulgosteren, Sukran Atikcan   +2 more
doaj   +1 more source

RT-PCR and RT-ddPCR primers.

, 2023
Abbreviations: F, forward; R, reverse; RT-PCR, reverse transcription-PCR; RT-ddPCR, reverse transcription-droplet digital PCR. (XLSX)
Patrizia Luppi (15307197), Peter Drain (15307200), Erik A. Koppes (15307191), Donna B. Stolz (6901796), John R. Yates III (8072333), Robert D. Nicholls (15307203), Hyun Jung Park (8778488), James J. Moresco (569744), Dale W. Lewis (6862094), Jolene K. Diedrich (1235706), Susanne M. Gollin (5063618), Simon C. Watkins (39900), Ronald C. Wek (15169871), Marie A. Johnson (15307194)   +13 more
core   +1 more source

Reproducibility in the Quantification of mRNA Levels by RT-PCR-ELISA and RT Competitive-PCR-ELISA

BioTechniques, 1998
The use of reverse transcription (RT) PCR for relative quantitation of gene transcripts relies on the reproducibility of the individual RT, PCR and product measurement steps.
L.L. Hall, G.R. Bicknell, L. Primrose, J.H. Pringle, J.A. Shaw, P.N. Furness   +5 more
doaj   +1 more source