Results 11 to 20 of about 890,017 (258)

RT-PCR Without RNA Isolation

BioTechniques, 1996
Reverse transcription-PCR (RT-PCR) has traditionally required time-consuming RNA extraction and purification. This report demonstrates that one can completely avoid the RNA extraction step in RT-PCR by basing the comparison of samples on cell number ...
R.J. Klebe, G.M. Grant, A.M. Grant, M.A. Garcia, T.A. Giambernardi, G.P. Taylor   +5 more
doaj   +1 more source

Reproducibility in the Quantification of mRNA Levels by RT-PCR-ELISA and RT Competitive-PCR-ELISA

BioTechniques, 1998
The use of reverse transcription (RT) PCR for relative quantitation of gene transcripts relies on the reproducibility of the individual RT, PCR and product measurement steps.
L.L. Hall, G.R. Bicknell, L. Primrose, J.H. Pringle, J.A. Shaw, P.N. Furness   +5 more
doaj   +1 more source

Development of an RNA Extraction Protocol for Norovirus from Raw Oysters and Detection by qRT-PCR and Droplet-Digital RT-PCR

Foods, 2021
Foodborne viruses such as norovirus and hepatitis A virus cause frequent outbreaks associated with the consumption of raw or undercooked oysters. Viral particles are bioaccumulated in the oyster’s digestive glands, making RNA extraction and RT-PCR ...
Daniel Plante, Julio Alexander Bran Barrera, Maude Lord, Irène Iugovaz, Neda Nasheri   +4 more
doaj   +1 more source

Application of standard and molecular methods for the diagnosis of newcastle disease [PDF]

Archives of Biological Sciences, 2012
Four pooled samples of whole poultry carcasses with their internal organs were used to determine the presence of Newcastle disease (ND) virus. Samples were collected from one epizootiological area in the Republic of Serbia during January 2007 ...
Vidanović D., Šekler M., Polaček V., Vasković N., Ašanin Ružica, Milić N., Nišavić J.   +6 more
doaj   +1 more source

Direct In Situ RT-PCR [PDF]

, 2011
In situ polymerase chain reaction (PCR) is a histological technique that exploits the advantages of PCR for detection of mRNA directly in tissue sections. It somehow conjugates together PCR and in situ hybridization that is more traditionally employed for mRNA localization in cell organelles, intact cells, or tissue sections. This chapter describes the
Laura, Lossi, Graziana, Gambino, Chiara, Salio, Adalberto, Merighi   +3 more
openaire   +2 more sources

Molekularbiologische Diagnostik von Pflanzenkrankkeiten

Julius-Kühn-Archiv, 2014
In der Abteilung für Molekularbiologische Diagnose von Pflanzenkrankheiten der AGES werden molekularbiologische Diagnoseverfahren eingesetzt um gesetzliche Kontroll- und Vollzugsaufgaben zu erfüllen, aber auch um Untersuchungen auf pflanzliche ...
Grausgruber-Gröger, Sabine, Gottsberger, Richard A., Leichtfried, Thomas, Reisenzein, Helga   +3 more
doaj   +1 more source

Optimisation of COVID‐19 diagnostic pathways in acute hospital admissions to prevent nosocomial transmission

The Clinical Respiratory Journal, 2022
Introduction In the management of acute hospital admissions during the COVID‐19 pandemic, safe patient cohorting depends on robust admission diagnostic strategies.
Robert Livingstone, Alexander Woodhead, Megha Bhandari, James Dias, Trevor Smith, Tom Havelock, Matthew Stammers   +6 more
doaj   +1 more source

Detecção de ácidos nucléicos de Brucella spp., Leptospira spp., herpesvirus bovino e vírus da diarréia viral bovina, em fetos bovinos abortados e em animais mortos no perinatal Detection of Brucella spp., Leptospira spp., bovine herpesvirus and bovine viral diarrhea virus nucleic acids in aborted fetuses and bovines dead perinatal

Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 2006
Samples of 114 bovine fetuses and 10 calves, which dead in perinatal period, were examined for detection of DNA. The most common detected agent was Brucella spp. in 17 samples (13.7%) followed by Leptospira spp. in 4 cases (3.2%),bovine herpesvirus (BHV)
A. Cortez, A.M.G. Castro, M.B. Heinemann, R.M. Soares, R.C. Leite, E. Scarcelli, M.E. Genovez, A.A. Alfieri, L.J. Richtzenhain   +8 more
doaj   +1 more source

Performance of an alternative RT-PCR procedure using residual sample from the Panbio™ Ag COVID-19 test

Brazilian Journal of Infectious Diseases, 2021
Introduction: In the current standard of care (SoC) RT-PCR method for COVID-19, the patient's swab was extracted in viral transport media (VTM). For the Panbio™ COVID-19 Ag Rapid Test, the patient swab is flushed out in extraction buffer, of which a ...
Terezinha Marta Pereira Pinto Castineiras, Érica Ramos dos Santos Nascimento, Débora Souza Faffe, Rafael Mello Galliez, Diana Mariani, Isabela de Carvalho Leitão, Mayla Gabryele Miranda de Melo, Orlando Costa Ferreira, Amilcar Tanuri   +8 more
doaj   +1 more source

BREAST CANCER TYPING USING RT-PCR ASSAY

Сибирский онкологический журнал, 2019
Introduction. Adjuvant systemic therapy remains one of the main options for treating breast cancer. Results of standard immunohistochemical studies are not always a criterion for selecting systemic therapy.
V. K. Bozhenko, I. D. Trotsenko, E. A. Kudinova, S. G. Vardanyan, M. V. Zakharenko, V. A. Solodky, M. V. Makarova   +6 more
doaj   +1 more source