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Establishment of DAS-ELISA for the detection of antigenic changes in glycinin after heat processing
International Journal of Biological Macromolecules, 2022In this study, a double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) method was established to detect the antigenic changes of thermally processed products containing glycinin. The proposed DAS-ELISA method used heat-treated antigen-absorbing antiserum as the coating antibody, and horseradish peroxidase (HRP)-labeled rabbit anti ...
Jun, Xi +5 more
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Development of a DAS-ELISA for detection of H9N2 avian influenza virus
Journal of Virological Methods, 2019H9N2 avian influenza virus is threatening animals and public health systems. Effective diagnosis is imperative to control the disease. Thus, we developed a panel of monoclonal antibodies (Mabs) against the H9N2 avian influenza virus (AIV) and implemented a double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) to detect the H9 viral ...
Fan Ming +4 more
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Entwicklung des DAS-ELISA fýr ausgewählte rhizosphärenbakterien
Zentralblatt für Mikrobiologie, 1991Summary Antisera against two Rhizobium strains (from pea and lucerne) and against a free living N2-fixation Klebsiella strain were produced and examinated for their sensitivity and specifity in immundiffusion and ELISA. The results show that the antiserum against the Klebsiella strain has an higher antibody titre than these against the rhizobia.
C. Scholz, R. Remus, R. Zielke
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Journal of Fish Diseases, 2021
AbstractKoi herpesvirus disease (KHVD) caused by the koi herpesvirus (KHV) is difficult to diagnose in live fish, presenting a challenge to the koi industry. The enzyme‐linked immunosorbent assay (ELISA) method cannot be widely used to detect KHV because few commercial anti‐KHV antibody exists.
Yingying Li +7 more
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AbstractKoi herpesvirus disease (KHVD) caused by the koi herpesvirus (KHV) is difficult to diagnose in live fish, presenting a challenge to the koi industry. The enzyme‐linked immunosorbent assay (ELISA) method cannot be widely used to detect KHV because few commercial anti‐KHV antibody exists.
Yingying Li +7 more
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1998
This exercise is meant to establish the concentration of a virus in sap of an infected plant. As an example, cowpea mosaic virus Comovirus (CPMV) in cowpea (Vigna unguiculata) has been chosen.
Jeanne Dijkstra, Cees P. de Jager
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This exercise is meant to establish the concentration of a virus in sap of an infected plant. As an example, cowpea mosaic virus Comovirus (CPMV) in cowpea (Vigna unguiculata) has been chosen.
Jeanne Dijkstra, Cees P. de Jager
openaire +1 more source
Development and Evaluation of a DAS-ELISA for Rapid Detection of Avian Influenza Viruses
Avian Diseases, 2006Rapid detection of avian influenza virus (AIV) infection is critical for control of avian influenza (AI) and for reducing the risk of pandemic human influenza. A double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) was developed for this purpose.
Anding, Zhang +7 more
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