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Journal of Fish Diseases, 2021
AbstractKoi herpesvirus disease (KHVD) caused by the koi herpesvirus (KHV) is difficult to diagnose in live fish, presenting a challenge to the koi industry. The enzyme‐linked immunosorbent assay (ELISA) method cannot be widely used to detect KHV because few commercial anti‐KHV antibody exists.
Yingying Li, Qing Wang, Feng Hu
exaly +3 more sources
AbstractKoi herpesvirus disease (KHVD) caused by the koi herpesvirus (KHV) is difficult to diagnose in live fish, presenting a challenge to the koi industry. The enzyme‐linked immunosorbent assay (ELISA) method cannot be widely used to detect KHV because few commercial anti‐KHV antibody exists.
Yingying Li, Qing Wang, Feng Hu
exaly +3 more sources
Development and Evaluation of a DAS-ELISA for Rapid Detection of Avian Influenza Viruses
Avian Diseases, 2006Rapid detection of avian influenza virus (AIV) infection is critical for control of avian influenza (AI) and for reducing the risk of pandemic human influenza. A double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) was developed for this purpose.
Anding Zhang, Meilin Jin, Yadi Tan
exaly +3 more sources
Development of a DAS-ELISA for detection of H9N2 avian influenza virus
Journal of Virological Methods, 2019H9N2 avian influenza virus is threatening animals and public health systems. Effective diagnosis is imperative to control the disease. Thus, we developed a panel of monoclonal antibodies (Mabs) against the H9N2 avian influenza virus (AIV) and implemented a double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) to detect the H9 viral ...
Sizhu Suolang, HongBo Zhou
exaly +3 more sources
Entwicklung des DAS-ELISA fýr ausgewählte rhizosphärenbakterien
Zentralblatt Für Mikrobiologie, 1991Summary Antisera against two Rhizobium strains (from pea and lucerne) and against a free living N2-fixation Klebsiella strain were produced and examinated for their sensitivity and specifity in immundiffusion and ELISA. The results show that the antiserum against the Klebsiella strain has an higher antibody titre than these against the rhizobia.
C. Scholz, R. Remus, R. Zielke
exaly +2 more sources
Archives of Phytopathology and Plant Protection, 2004
DAS-ELISA studies were conducted on detection of sugarcane yellow leaf virus (SCYLV) causing yellow leaf syndrome (YLS) of sugarcane in leaf and juice antigens. Among the two types of antigen sources used for the virus detection, juice antigen showed high titre for the virus as compared to leaf antigen.
R Viswanathan
exaly +2 more sources
DAS-ELISA studies were conducted on detection of sugarcane yellow leaf virus (SCYLV) causing yellow leaf syndrome (YLS) of sugarcane in leaf and juice antigens. Among the two types of antigen sources used for the virus detection, juice antigen showed high titre for the virus as compared to leaf antigen.
R Viswanathan
exaly +2 more sources
Comparison of DAS‐ELISA and qRT‐PCR for the detection of cucurbit viruses in seeds
Annals of Applied Biology, 2019AbstractThe importance of seeds as virus vehicles for long‐distance dissemination makes essential the availability of adequate methods of analysis to guarantee the quality of seed lots. To improve the repertoire of sensitive methods for seed diagnosis, we have developed quantitative real‐time RT‐PCR assays (RT‐qPCR) based on the TaqMan technology to ...
Covadonga Torre +3 more
openaire +1 more source
1998
This exercise is meant to establish the concentration of a virus in sap of an infected plant. As an example, cowpea mosaic virus Comovirus (CPMV) in cowpea (Vigna unguiculata) has been chosen.
Jeanne Dijkstra, Cees P. de Jager
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This exercise is meant to establish the concentration of a virus in sap of an infected plant. As an example, cowpea mosaic virus Comovirus (CPMV) in cowpea (Vigna unguiculata) has been chosen.
Jeanne Dijkstra, Cees P. de Jager
openaire +1 more source
Detection of Tomato Spotted Wilt Virus (TSWV) Infection in Plants Using DAS-ELISA and Dot-ELISA
2021Plant viruses cause severe damages to crop productions each year worldwide. To prevent the losses caused by plant viruses, it is necessary to develop specific and efficient diagnostic tools to detect viruses. Among the current virus detection techniques, serological detection methods are considered to be rapid, simple, sensitive, and high throughput ...
Shibo, Gao, Jianxiang, Wu
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CHARACTERIZATION OF PLUM POX POTYVIRUS (PPV) BY DAS-ELISA AND RT-PCR/RFLP ANALYSIS IN TURKEY
Acta Horticulturae, 2004This study was conducted to determine the presence of Plum pox virus (PPV) (family Potyviridae, genus Potyvirus) in different regions of Turkey and to characterise PPV isolates by serological and molecular techniques including ELISA, PCR/ RFLP. Thus, leaf samples from different stone fruit species, almond, apricot, nectarine, peach, plum, sour and ...
Caglayan, K +4 more
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Journal of Phytopathology, 1991
AbstractSingle and mixed antisera have been compared in DAS‐ELISA for the routine diagnosis of nepoviruses infecting grapevine. The use of mixed polyclonal antibodies allowed in a single test the simultaneous detection of several nepoviruses (ArMV + GFLV) or serotypes of nepoviruses (TBRV serotypes G + S and RRV serotypes E + G) whatever the nature of ...
Etienne, Lucien +2 more
openaire +2 more sources
AbstractSingle and mixed antisera have been compared in DAS‐ELISA for the routine diagnosis of nepoviruses infecting grapevine. The use of mixed polyclonal antibodies allowed in a single test the simultaneous detection of several nepoviruses (ArMV + GFLV) or serotypes of nepoviruses (TBRV serotypes G + S and RRV serotypes E + G) whatever the nature of ...
Etienne, Lucien +2 more
openaire +2 more sources