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Comparison of DAS‐ELISA and qRT‐PCR for the detection of cucurbit viruses in seeds
Annals of Applied Biology, 2019AbstractThe importance of seeds as virus vehicles for long‐distance dissemination makes essential the availability of adequate methods of analysis to guarantee the quality of seed lots. To improve the repertoire of sensitive methods for seed diagnosis, we have developed quantitative real‐time RT‐PCR assays (RT‐qPCR) based on the TaqMan technology to ...
Covadonga Torre +3 more
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1998
This exercise is meant to establish the concentration of a virus in sap of an infected plant. As an example, cowpea mosaic virus Comovirus (CPMV) in cowpea (Vigna unguiculata) has been chosen.
Jeanne Dijkstra, Cees P. de Jager
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This exercise is meant to establish the concentration of a virus in sap of an infected plant. As an example, cowpea mosaic virus Comovirus (CPMV) in cowpea (Vigna unguiculata) has been chosen.
Jeanne Dijkstra, Cees P. de Jager
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Detection of Tomato Spotted Wilt Virus (TSWV) Infection in Plants Using DAS-ELISA and Dot-ELISA
2021Plant viruses cause severe damages to crop productions each year worldwide. To prevent the losses caused by plant viruses, it is necessary to develop specific and efficient diagnostic tools to detect viruses. Among the current virus detection techniques, serological detection methods are considered to be rapid, simple, sensitive, and high throughput ...
Shibo, Gao, Jianxiang, Wu
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CHARACTERIZATION OF PLUM POX POTYVIRUS (PPV) BY DAS-ELISA AND RT-PCR/RFLP ANALYSIS IN TURKEY
Acta Horticulturae, 2004This study was conducted to determine the presence of Plum pox virus (PPV) (family Potyviridae, genus Potyvirus) in different regions of Turkey and to characterise PPV isolates by serological and molecular techniques including ELISA, PCR/ RFLP. Thus, leaf samples from different stone fruit species, almond, apricot, nectarine, peach, plum, sour and ...
Caglayan, K +4 more
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Journal of Phytopathology, 1991
AbstractSingle and mixed antisera have been compared in DAS‐ELISA for the routine diagnosis of nepoviruses infecting grapevine. The use of mixed polyclonal antibodies allowed in a single test the simultaneous detection of several nepoviruses (ArMV + GFLV) or serotypes of nepoviruses (TBRV serotypes G + S and RRV serotypes E + G) whatever the nature of ...
Etienne, Lucien +2 more
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AbstractSingle and mixed antisera have been compared in DAS‐ELISA for the routine diagnosis of nepoviruses infecting grapevine. The use of mixed polyclonal antibodies allowed in a single test the simultaneous detection of several nepoviruses (ArMV + GFLV) or serotypes of nepoviruses (TBRV serotypes G + S and RRV serotypes E + G) whatever the nature of ...
Etienne, Lucien +2 more
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Journal of Phytopathology, 1996
AbstractIn order to develop a reliable diagnostic test for pepper veinal mottle virus (PVMV), a panel of monoclonal antibodies (mAbs), raised to a Nigerian isolate (PVMW‐Ni), was evaluated by double‐antibody sandwich enzyme‐linked immunosorbent assay (DAS‐ELISA) on infected pepper samples from seven West African countries.
C. Huguenot +3 more
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AbstractIn order to develop a reliable diagnostic test for pepper veinal mottle virus (PVMV), a panel of monoclonal antibodies (mAbs), raised to a Nigerian isolate (PVMW‐Ni), was evaluated by double‐antibody sandwich enzyme‐linked immunosorbent assay (DAS‐ELISA) on infected pepper samples from seven West African countries.
C. Huguenot +3 more
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Detekce virů pícnin pomocí DAS-ELISA
2014The subject of this bachelor thesis is screening of viruses on wild and cultivated forage crop growing in the Czech Republic. Two viruses Red clover vein mosaic virus (RCVMV; genus Carlavirus) and Clover yellow mosaic virus (ClYMV; genus Potexvirus) were screened using the DAS-ELISA. Three plants of Trifolium pratense (variety Amos, Blizard and Rezista)
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Plant Disease, 1995
Skaf, J. S., and Carroll, T. W. 1995. Purification of barley yellow streak mosaic virus and detection by DAS-ELISA and ISEM using polyclonal antibodies. Plant Dis. 79:1003-1007. A purification procedure was developed for barley yellow streak mosaic virus (BaYSMV), the causal agent of a disease in barley and wheat.
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Skaf, J. S., and Carroll, T. W. 1995. Purification of barley yellow streak mosaic virus and detection by DAS-ELISA and ISEM using polyclonal antibodies. Plant Dis. 79:1003-1007. A purification procedure was developed for barley yellow streak mosaic virus (BaYSMV), the causal agent of a disease in barley and wheat.
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2017
The present study was conducted to compare the sensibility of differentmethods (DAS-ELISA and RT-PCR) which are used to detect the agents of viraldiseases in grapevine. Purposive sampling was done in vineyards in Manisa andİzmir provinces and a total of 50 samples were taken from grapevines showingvirus symptoms.
ERİLMEZ, Serpil, KAYA, Aydan
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The present study was conducted to compare the sensibility of differentmethods (DAS-ELISA and RT-PCR) which are used to detect the agents of viraldiseases in grapevine. Purposive sampling was done in vineyards in Manisa andİzmir provinces and a total of 50 samples were taken from grapevines showingvirus symptoms.
ERİLMEZ, Serpil, KAYA, Aydan
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Journal of fish diseases
Anguillid herpesvirus (AngHV) is a highly pathogenic agent that causes "Mucus sloughing and hemorrhagic septicemia disease" in eels, resulting in high morbidity and cumulative mortality rates. Therefore, accurate diagnosis of AngHV infection is essential for effective clinical management and epidemiological control.
Hua, Chen, Jin-Xian, Yang, Jun-Qing, Ge
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Anguillid herpesvirus (AngHV) is a highly pathogenic agent that causes "Mucus sloughing and hemorrhagic septicemia disease" in eels, resulting in high morbidity and cumulative mortality rates. Therefore, accurate diagnosis of AngHV infection is essential for effective clinical management and epidemiological control.
Hua, Chen, Jin-Xian, Yang, Jun-Qing, Ge
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