Results 81 to 90 of about 2,403,047 (268)

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Nationwide Trends and Safety Analysis of Adult Spinal Instrumentation Surgery: A Study using the JSIS-DB

Spine Surgery and Related Research
Introduction: Spinal instrumentation surgery is increasingly being performed in aging populations; however, comprehensive data regarding the nationwide trends and perioperative safety associated with these procedures in Japan remain limited.
Hideyuki Arima, Haruki Ueda, Shunsuke Katsumi, Yusuke Hori, Junya Katayanagi, Atsushi Tagami, Mitsuru Yagi, Ryo Mizukoshi, Koji Yamada, Kimiaki Yokosuka, Yukihiro Matsuyama, Hiroshi Taneichi, Tokumi Kanemura, Akira Matsumura   +13 more
doaj  

Interaction of Dislocations and Interfaces in Crystalline Heterostructures: A Review of Atomistic Studies

Crystals, 2019
Interfaces in heterostructures of crystalline materials could strongly affect the slip of dislocations. Such interfaces have become one of the most popular methods to tailor material strength and ductility.
Zhibo Zhang, Cancan Shao, Shuncheng Wang, Xing Luo, Kaihong Zheng, Herbert M. Urbassek   +5 more
doaj   +1 more source

The role of miR‐335‐5p in the redifferentiation of BRAF p.V600E thyroid cancers

Molecular Oncology, EarlyView.
The BRAF p.V600E mutation promotes thyroid cancer dedifferentiation and radioiodine resistance. Using a network approach, we identified miR‐335‐5p as a key regulator of BRAF‐mutated thyroid tumors. Restoring miR‐335‐5p increased thyroid‐specific gene expression and iodine uptake in cells and organoids.
Valeria Pecce, Simone Bini, Marialuisa Sponziello, Giorgio Grani, Giulia Fiscon, Paola Paci, Lorenzo Farina, Rosa Falcone, Valentina Maggisano, Sebastiano Filetti, Cosimo Durante, Antonella Verrienti   +11 more
wiley   +1 more source

Subtype‐specific enhancer RNAs define transcriptional regulators and prognosis in breast cancers

Molecular Oncology, EarlyView.
This study employed machine learning methodologies to perform the subtype‐specific classification of RNA‐seq data sets, which are mapped on enhancers from TCGA‐derived breast cancer patients. Their integration with gene expression (referred to as ProxCReAM eRNAs) and chromatin accessibility profiles has the potential to identify lineage‐specific and ...
Aamena Y. Patel, Peyman Zarrineh, Nathnael T. Tuffa, Jigar H. Sheth, Sumitra Mohan, Mudassar Iqbal, Sankari Nagarajan   +6 more
wiley   +1 more source

Establishment and Preliminary Outcomes of a Nationwide Spine and Spinal Cord Surgery Registry in Japan: Analysis of 158,263 Cases in 2022

Spine Surgery and Related Research
Introduction: The Japanese Society for Spine Surgery and Related Research (JSSR) developed a national web-based registry system, integrating it with the Japanese Orthopaedic Association National Registry (JOANR/JSSR-DB) to systematically collect data on ...
Hideyuki Arima, Chie Shimmura, Yosuke Shibata, Toshimi Aizawa, Nobuyuki Fujita, Takanori Fujita, Naobumi Hosogane, Shota Ikegami, Shiro Imagama, Gen Inoue, Ken Ishii, Yukihiro Nakagawa, Hiroaki Nakashima, Seiji Ohtori, Haruki Ueda, Kei Watanabe, Mitsuru Yagi, Koji Yamada, Toshitaka Yoshii, Hirotaka Chikuda, Hirotaka Haro, Yukihiro Matsuyama, Masaya Nakamura, Hiroshi Taneichi, Tokumi Kanemura   +24 more
doaj  

COMP–PMEPA1 axis promotes epithelial‐to‐mesenchymal transition in breast cancer cells

Molecular Oncology, EarlyView.
This study reveals that cartilage oligomeric matrix protein (COMP) promotes epithelial‐to‐mesenchymal transition (EMT) in breast cancer. We identify PMEPA1 (protein TMEPAI) as a novel COMP‐binding partner that mediates EMT via binding to the TSP domains of COMP, establishing the COMP–PMEPA1 axis as a key EMT driver in breast cancer.
Konstantinos S. Papadakos, Gilar Gorji‐bahri, Lejla Gradjan, Julia Zajac, Kacper Gil, Yvonne Thokozile Nduku, Anna M. Blom   +6 more
wiley   +1 more source

Database Cracking. [PDF]

, 2007
Database indices provide a non-discriminative navigational infrastructure to localize tuples of interest. Their maintenance cost is taken during database updates. In this paper, we study the complementary approach, addressing index maintenance as part of query processing using continuous physical reorganization, i.e., cracking the database into ...
S. Idreos (Stratos), M.L. Kersten (Martin), S. Manegold (Stefan)   +2 more
openaire   +2 more sources

Pre‐analytical optimization of cell‐free DNA and extracellular vesicle‐derived DNA for mutation detection in liquid biopsies

Molecular Oncology, EarlyView.
Pre‐analytical handling critically determines liquid biopsy performance. This study defines practical best‐practice conditions for cell‐free DNA (cfDNA) and extracellular vesicle–derived DNA (evDNA), showing how processing time, storage conditions, tube type, and plasma input volume affect DNA integrity and mutation detection.
Jonas Dohmen, Lucrezia De Santis, Bret M. Stephens, Vincent Bernard, Jörg C. Kalff, Lara Braun, Phillipp Leyendecker, Johannes Röttgen, Daniel Weissinger, Anirban Maitra, Paola A. Guerrero, Alexander Semaan   +11 more
wiley   +1 more source

EDNRB‐dependent endothelin signaling reduces proliferation and promotes proneural‐to‐mesenchymal transition in gliomas

Molecular Oncology, EarlyView.
Glioma cells mainly express the endothelin receptor EDNRB, while EDNRA is restricted to a perivascular tumor subpopulation. Endothelin signaling reduces glioma cell proliferation while promoting migration and a proneural‐to‐mesenchymal transition associated with poor prognosis. This pathway activates Ca2+, K+, ERK, and STAT3 signalings and is regulated
Donovan Pineau, Leonor Garcia, Hugo Arnold, Antonija Hanžek, Maialen Arrieta, Laurent R Gauthier, Christine Granotier‐Beckers, François D Boussin, Amaury Herbet, Marie Hautière, Valentin Asei‐Ceschino, Clémentin Jacques, Laura Brard, Thomas Harnois, Valérie Coronas, Bruno Constantin, Aurélien Chatelier, Jean Chemin, Serge Urbach, Martial Seveno, Szimonetta Hideg, Chantal Ripoll, Kasandra Aguilar‐Cázarez, Min Zheng, Guo‐Hao Huang, Sheng‐Qing Lv, Lei Zhang, Philippe Rondard, Laurent Prezeau, Jean‐Philippe Pin, Hugues Duffau, Luc Bauchet, Valérie Rigau, Franck Denat, Charles Truillet, Didier Boquet, Jean‐Philippe Hugnot   +36 more
wiley   +1 more source