Results 131 to 140 of about 20,716 (184)

Fractionation of mammalian DNA on DEAE-cellulose

Journal of Chromatography A, 1975
Chromatography on a DEAE-cellulose (DE-52) column of native and denatured DNA from P388F cells has been studied. The main bulk of native DNA is eluted at 0.8M NaC1 and a minor fraction is eluted with 0.5N NaOH. The proportion of the DNA components obtained depends on the type of isotopic labelling used and the method of storing of the DNA preparation ...
Fiona Millard, Brian W. Fox
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Adsorption of Glucoamylase on DEAE‐Cellulose

Starch - Stärke, 1983
AbstractThe adsorption of glucoamylase on DEAE‐cellulose and the properties of the immobilized enzyme in respect to its use in hydrolysis of starch are described.
M. Jach, H. Sugier
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DEAE cellulose chromatographic characterization of canine serum

Comparative Immunology, Microbiology and Infectious Diseases, 1980
Abstract Canine serum samples were fractionated on DEAE cellulose using a continuous gradient system to establish a representative chromatographic pattern for normal dog serum. Serum samples were separated using decreasing pH (8.4–4.5) and increasing molarity (0.01–0.3 m ) phosphate gradient and the elution pattern was obtained by spectrophotometric
C C, Whitacre, V S, Mehl, R W, Lang
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DEAE-Cellulose Chromatography of Kidney Worm Antigens

The Journal of Parasitology, 1963
From extracts of one collection of kidney worms, nine components were separated; eight contained antigens, some of which were common to all. From a second collection, four components absorbing strongly at 280 mje (and presumed to be largely protein) and seven carbohydrate components were separated; three of the former and five of the latter contained ...
L A, BAISDEN, F G, TROMBA
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Separation of Heparin into Subtractions by DEAE-Cellulose Chromatography

Thrombosis and Haemostasis, 1979
SummaryCommercial porcine heparin can be separated into three distinct subtractions by using DEAE-cellulose chromatography and a stepped salt gradient. Gram quantities of heparin can be fractionated by this technique. All three heparin subtractions can accelerate the inhibition of thrombin by antithrombin III with different efficiency.
R H, Yue, T, Starr, M M, Gertler
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Methods for the separation and identification of ribonucleotides on DEAE-cellulose

Analytical Biochemistry, 1973
Abstract Methods have been developed for the separation and identification of ribonucleotides. These methods include single gradient column chromatography with DEAE-cellulose type DE52 to separate purine and pyrimidine ribonucleotides, and PEI-cellulose to separate purine ribonucleotides. Sequential thin layer chromatography with PEI-cellulose may be
Y M, Rustum, H S, Schwartz
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Chromatography of acidic glycosaminoglycans on DEAE-cellulose

Journal of Chromatography A, 1972
Abstract An ion-exchange procedure has been developed for the separation and analysis of milligram amounts of acidic glycosaminoglycans. The polysaccharides were first applied to a column of Sephadex G-50 and eluted with 0.15 M sodium chloride.
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Characteristics of DNA Fractionated on Benzoylated DEAE-Cellulose

Hoppe-Seyler´s Zeitschrift für physiologische Chemie, 1975
Chromatography on BD-cellulose columns with a salt gradient and formamide separates cellular DNA into two fractions (fraction I eluted within the salt gradient, fraction II with formamide), the proportions of these two fractions (ca. 2:1) being similar for DNA from a number of eucaryotic organisms.
G, Pirro, H, Feldmann
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Fractionation of nucleic acid on benzoylated-naphthoylated DEAE cellulose

Journal of Molecular Biology, 1967
Nucleic acids have been fractionated by column chromatography using methylated albumin kieselguhr (Mandell & Hershey, 1960; Sueoka & Cheng, 1962) cellulose CF-11 (Franklin, 1966), agarose (Erikson & Gordon, 1966) and hydroxyapatite (Muench & Berg, 1966).
Sedat, John W., Kelly, Regis B., Sinsheimer, Robert L.   +2 more
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The purification of phosphate esters on DEAE-cellulose

Journal of Chromatography A, 1971
Abstract The utility of DEAE-cellulose for separating phosphate esters is examined. Emphasis is given to the fractionation of labile derivatives which cannot readily be isolated by conventional methods. The factors involved in resolving these phosphate derivatives from contaminants such as inorganic phosphate, sulphate, chloride and free sugars on ...
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