Results 141 to 150 of about 20,716 (184)

Chromatography of Blood Thrombokinase on ‘DEAE’-Cellulose

Nature, 1960
THROMBOKINASE, prepared from bovine plasma, has been obtained in a highly purified state by repeated electrophoretic fractionations1. However, a more efficient method was promised by anion-exchange chromatography, provided that the kinase could be separated from its accompanying impurities by stepwise elution in one day2.
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Heparin binding and release properties of DEAE cellulose membranes

Biomaterials, 1983
Two cellulose-based membranes, containing 5 and 20% N, N-diethyl-aminoethyl cellulose (DEAE) respectively, were investigated for ionic attachment of heparin and heparin release. Heparinization was achieved by recirculation of a heparin-containing glycine buffer over each membrane. Heparin was determined by chemical analysis or by 99mTc labelling.
E, Schmitt, M, Holtz, H, Klinkmann, G, Esther, J M, Courtney   +4 more
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A DEAE-cellulose filter disk assay for aminoacyl-tRNA

Analytical Biochemistry, 1974
Abstract A filtration assay is described for quantitation of aminoacylated tRNA. [ 3 H]Aminoacyl-tRNA is adsorbed on DEAE-cellulose filter disks under conditions in which unreacted amino acid is removed by a 100-m m glycine-HCl (pH 2.3) wash. The assay is rapid, simple to perform, and linear over the range of 0.005–20 A 260 units/50-μl filtered ...
D V, Santi, R T, Anderson
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Study of adenovirus antigens fractionated by chromatography on DEAE-cellulose

Virology, 1959
Abstract Partially purified preparations of adenovirus types 2 and 5 were fractionated by chromatography on DEAE-cellulose columns. Elution with increasing NaCl concentrations, yielded in succession the antigens previously designated C, B, and A, followed by the infective particle.
H G, KLEMPERER, H G, PEREIRA
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Isolation of IgG by DEAE Cellulose

1979
Weakly basic cellulose ion exchanges, such as DEAE cellulose, can be used for the separation of serum proteins according to the procedure of Sober and co-workers (1956). This procedure is especially useful for isolation of IgG antibodies, which leave the column in a relatively pure form right after the void volume.
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Utilisation of DEAE-cellulose as a microcarrier material.

Developments in biological standardization, 1980
Hela and Namalwa cells readily attach to, and grow on, the surface of DEAE-substituted cellulose fibres. Subcultivation of these cells can be accomplished either by trypsinisation or by contact transfer of cells from fibre to fibre. Attempts to establish an anchorage-preferring sub-population of Namalwa cells were unsuccessful.
P, Talbot, M J, Keen
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Batch preparation of rabbit γG globulin with DEAE-cellulose

Molecular Immunology, 1967
Abstract A rapid method for the preparatoin of γG globulin of specified purity from rabbit serum by a single batch absorption with DEAE-cellulose is described. The percentage purity of the product depends upon the quantity of DEAE-cellulose used per ml serum.
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Isolation of glucose oxidase with the use of DEAE‐cellulose

Journal of Applied Chemistry and Biotechnology, 1971
AbstractA method is described for the isolation of glucose oxidase from the bacteria Aspergillus niger using sorption on DEAE‐cellulose. The method is rapid by comparison with precipitation methods and results in a preparation having a specific activity of 87‐5 e.u./mg.
J. Kučera, J. Hanus
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Separation on DEAE cellulose of components associated with adenovirus reproduction

Virology, 1960
Abstract Crude tissue culture fluids from cells infected with adenovirus were separated into different fractions by chromatography on DEAE-cellulose. One fraction contained the protein factor responsible for the early cytopathic effect, a second contained the soluble complement-fixing antigen and about 15% of the infectivity.
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Molybdate Affects Glucocorticoid Receptor Chromatographyon Deae Cellulose

Journal of Receptor Research, 1986
How molybdate affects the chromatographic behavior of the glucocorticoid receptor on DEAE cellulose was investigated. Over the range 0-20 mM, molybdate caused a progressive change in the elution positions of both the transformed and untransformed receptor. At 20 mM molybdate, the transformed receptor eluted from the column before application of the KCl
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