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Sizing of single-stranded regions in double-stranded DNA by preparative benzoylated DEAE-cellulose chromatography

open access: yesFEBS Letters, 1988
The concentration of caffeine required to elute wholly single-stranded DNA from benzoylated DEAE-cellulose is proportional to the polynucleotide length. The use of benzoylated DEAE-cellulose chromatography for isolating and sizing single-stranded regions
Murray D Norris
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Limitations on the stability of benzoylated DEAE-cellulose

Analytical Biochemistry, 1984
As used for structural analysis of DNA, benzoylated DEAE-cellulose is subject to deterioration which affects chromatographic performance. This change, which specifically occurs during storage of the resin, has been characterized in terms of anomalous patterns of DNA binding.
M, Haber, P H, Huang, B W, Stewart
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Fractionation of DNA on benzoylated DEAE-cellulose

Analytical Biochemistry, 1975
Abstract Escherichia coli DNA separates into two fractions upon chromatography on columns of benzoylated-DEAE-cellulose (BD-cellulose). One fraction binds more strongly and only elutes in 1 m NaCl-1.8% caffeine solution, similar to the behavior of denatured DNA on such columns.
N A, Caffin, A G, Mackinlay
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Fractionation of mammalian DNA on DEAE-cellulose

Journal of Chromatography A, 1975
Chromatography on a DEAE-cellulose (DE-52) column of native and denatured DNA from P388F cells has been studied. The main bulk of native DNA is eluted at 0.8M NaC1 and a minor fraction is eluted with 0.5N NaOH. The proportion of the DNA components obtained depends on the type of isotopic labelling used and the method of storing of the DNA preparation ...
Fiona Millard, Brian W. Fox
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Adsorption of Glucoamylase on DEAE‐Cellulose

Starch - Stärke, 1983
AbstractThe adsorption of glucoamylase on DEAE‐cellulose and the properties of the immobilized enzyme in respect to its use in hydrolysis of starch are described.
M. Jach, H. Sugier
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DEAE cellulose chromatographic characterization of canine serum

Comparative Immunology, Microbiology and Infectious Diseases, 1980
Abstract Canine serum samples were fractionated on DEAE cellulose using a continuous gradient system to establish a representative chromatographic pattern for normal dog serum. Serum samples were separated using decreasing pH (8.4–4.5) and increasing molarity (0.01–0.3 m ) phosphate gradient and the elution pattern was obtained by spectrophotometric
C C, Whitacre, V S, Mehl, R W, Lang
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DEAE-Cellulose Chromatography of Kidney Worm Antigens

The Journal of Parasitology, 1963
From extracts of one collection of kidney worms, nine components were separated; eight contained antigens, some of which were common to all. From a second collection, four components absorbing strongly at 280 mje (and presumed to be largely protein) and seven carbohydrate components were separated; three of the former and five of the latter contained ...
L A, BAISDEN, F G, TROMBA
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Separation of Heparin into Subtractions by DEAE-Cellulose Chromatography

Thrombosis and Haemostasis, 1979
SummaryCommercial porcine heparin can be separated into three distinct subtractions by using DEAE-cellulose chromatography and a stepped salt gradient. Gram quantities of heparin can be fractionated by this technique. All three heparin subtractions can accelerate the inhibition of thrombin by antithrombin III with different efficiency.
R H, Yue, T, Starr, M M, Gertler
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