Results 181 to 190 of about 169,155 (228)

Methods for the separation and identification of ribonucleotides on DEAE-cellulose

Analytical Biochemistry, 1973
Abstract Methods have been developed for the separation and identification of ribonucleotides. These methods include single gradient column chromatography with DEAE-cellulose type DE52 to separate purine and pyrimidine ribonucleotides, and PEI-cellulose to separate purine ribonucleotides. Sequential thin layer chromatography with PEI-cellulose may be
Y M, Rustum, H S, Schwartz
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Chromatography of acidic glycosaminoglycans on DEAE-cellulose

Journal of Chromatography A, 1972
Abstract An ion-exchange procedure has been developed for the separation and analysis of milligram amounts of acidic glycosaminoglycans. The polysaccharides were first applied to a column of Sephadex G-50 and eluted with 0.15 M sodium chloride.
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Characteristics of DNA Fractionated on Benzoylated DEAE-Cellulose

Hoppe-Seyler´s Zeitschrift für physiologische Chemie, 1975
Chromatography on BD-cellulose columns with a salt gradient and formamide separates cellular DNA into two fractions (fraction I eluted within the salt gradient, fraction II with formamide), the proportions of these two fractions (ca. 2:1) being similar for DNA from a number of eucaryotic organisms.
G, Pirro, H, Feldmann
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The purification of phosphate esters on DEAE-cellulose

Journal of Chromatography A, 1971
Abstract The utility of DEAE-cellulose for separating phosphate esters is examined. Emphasis is given to the fractionation of labile derivatives which cannot readily be isolated by conventional methods. The factors involved in resolving these phosphate derivatives from contaminants such as inorganic phosphate, sulphate, chloride and free sugars on ...
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Chromatography of Blood Thrombokinase on ‘DEAE’-Cellulose

Nature, 1960
THROMBOKINASE, prepared from bovine plasma, has been obtained in a highly purified state by repeated electrophoretic fractionations1. However, a more efficient method was promised by anion-exchange chromatography, provided that the kinase could be separated from its accompanying impurities by stepwise elution in one day2.
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Heparin binding and release properties of DEAE cellulose membranes

Biomaterials, 1983
Two cellulose-based membranes, containing 5 and 20% N, N-diethyl-aminoethyl cellulose (DEAE) respectively, were investigated for ionic attachment of heparin and heparin release. Heparinization was achieved by recirculation of a heparin-containing glycine buffer over each membrane. Heparin was determined by chemical analysis or by 99mTc labelling.
E, Schmitt, M, Holtz, H, Klinkmann, G, Esther, J M, Courtney   +4 more
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A DEAE-cellulose filter disk assay for aminoacyl-tRNA

Analytical Biochemistry, 1974
Abstract A filtration assay is described for quantitation of aminoacylated tRNA. [ 3 H]Aminoacyl-tRNA is adsorbed on DEAE-cellulose filter disks under conditions in which unreacted amino acid is removed by a 100-m m glycine-HCl (pH 2.3) wash. The assay is rapid, simple to perform, and linear over the range of 0.005–20 A 260 units/50-μl filtered ...
D V, Santi, R T, Anderson
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Study of adenovirus antigens fractionated by chromatography on DEAE-cellulose

Virology, 1959
Abstract Partially purified preparations of adenovirus types 2 and 5 were fractionated by chromatography on DEAE-cellulose columns. Elution with increasing NaCl concentrations, yielded in succession the antigens previously designated C, B, and A, followed by the infective particle.
H G, KLEMPERER, H G, PEREIRA
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Isolation of IgG by DEAE Cellulose

1979
Weakly basic cellulose ion exchanges, such as DEAE cellulose, can be used for the separation of serum proteins according to the procedure of Sober and co-workers (1956). This procedure is especially useful for isolation of IgG antibodies, which leave the column in a relatively pure form right after the void volume.
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Utilisation of DEAE-cellulose as a microcarrier material.

Developments in biological standardization, 1980
Hela and Namalwa cells readily attach to, and grow on, the surface of DEAE-substituted cellulose fibres. Subcultivation of these cells can be accomplished either by trypsinisation or by contact transfer of cells from fibre to fibre. Attempts to establish an anchorage-preferring sub-population of Namalwa cells were unsuccessful.
P, Talbot, M J, Keen
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