Two water‐soluble polysaccharides were isolated and purified from Tricholoma flavovirens (TF‐P) and Trametes versicolor (TV‐P), respectively. The structures of the two polysaccharides were identified by combining GPC, FT‐IR, HPLC, GC–MS, and NMR, and the structure–activity relationship has also been studied using immune cells and tumor cells. TV‐P with
Zheng‐Ao Sun +5 more
wiley +1 more source
Purification and characterization of an extracellular amylase from Lactobacillus plantarum strain A6 [PDF]
Extracellular amylase from #Lactobacillus plantarum$ A6 was purified by fractionated precipitation with ammonium sulphate and by anion exchange chromatography.
Giraud, Eric +4 more
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Chromatography of McCaslan Pole Bean Hemagglutinin on DEAE-Cellulose
SummaryA crude preparation of McCaslan Pole Bean phytohemagglutinin has been fractionated chromatographically on DEAE-cellulose. Hemagglutinating activity, followed by testing each fraction with an rbc panel, was found in 3 groups of fractions. In each of these groups the activity was panagglutinating and gave the same titers with each rbc used in ...
M D, PRAGER, R J, SPEER
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Anti-Platelet Fraction Isolated from Galega Officinalis
A fraction from crude extract of Galega officinalis has been purified by column chromatography on Sephadex G-25, Sepharose 4B, DEAE-Cellulose and Sephadex G-100. The final purification factor of the fraction is 120.
Atanasov A.
doaj +1 more source
Prospective application of diethylaminoethyl cellulose (DEAE-cellulose) with a high adsorption capacity toward the detoxification of 2,4-dichlorophenoxyacetic acid (2,4-D) from water. [PDF]
Kodali J +4 more
europepmc +1 more source
Chromatography of Acidic Polysaccharides on Deae-Cellulose [PDF]
The chromatography of polysaccharides on resinous ion-exchangers has been reported by Steiner, Neukom, and Deuel (1958). Neukom et al. (1960) later reported the chromatography of a variety of wheat and sugar-beet polysaccharides on DEAEcellulose. Only "neutral" polysaccharides could be eluted at pH 6-8 by increasing buffer and salt concentrations; acid
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In the present study, the fruiting bodies of Cordyceps militaris were used as raw materials to extract the crude polysaccharides Y-1, Y-2, Y-3, Y-4, Y-5, Y-6 and Y-7, which fractional precipitation using the ethanol with different volume fractions of 30%,
Yanan WANG +11 more
doaj +1 more source
A comparative study of an aminopeptidase from lactic acid bacteria: a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Biochemistry at Massey University [PDF]
Aminopeptidase enzymes from the proteolytic systems of S.salivarius subsp.thermophilus Lactococcus lactis subsp.cremoris and Lactococcus lactis subsp.lactis have been investigated.
Midwinter, Robyn Gillian
core
A monoclonal antibody that blocks the activity of a neurite regeneration-promoting factor: studies on the binding site and its localization in vivo [PDF]
Work from several laboratories has identified a proteoglycan complex secreted by a variety of non-neuronal cells that can promote neurite regeneration when applied to the surface of culture dishes.
Chiu, Arlene Y. +2 more
core +1 more source
Purification of Rennet by DEAE-Cellulose Chromatography
レンネットをDEAE-セルロースカラムに吸着させ, 0.1M燐酸緩衝液(pH 5.7)に0.1, 0.2および0.3M NaClを加えて調製した溶出液および0.1N HClで順次溶出させてレンニンを分離精製し,あわせてレンネット中に混在するペプシンをも分離し,その活性割合を測定した. (1) 0.1M燐酸緩衝液(pH 5.7)に添加するNaClの濃度を0.1Mから0.2Mに増加した溶出液で得られるFraction 3に凝乳力カが存在し, pH 6.62,イオン強度0.2の燐酸緩衝液を用いた電気泳動,沈降分析からみて均一性の高いレンニンが得られた.
Umeo YOSHINO +3 more
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