Results 1 to 10 of about 21,128 (261)
Identification of Optimal Decalcification Method and Tissue Preparation Protocol for RNAscope In Situ Hybridization in Rodent Incisor Tooth [PDF]
Dentistry JournalBackground: RT-qPCR is the gold standard for quantitative gene expression analysis, but it requires homogenized tissue and thus loses spatial information.
János Konkoly +10 more
doaj +2 more sources
Exploring the aluminium paradox in calcium-aluminosilicate-hydrate decalcification [PDF]
Nature CommunicationsDecalcification, a prevalent form of mineral dissolution, drives the long-term degradation of cementitious materials in civil and energy infrastructures and controls the weathering of silicates in Earth’s crust. The impact of aluminium (Al) incorporation
Yong Tao +4 more
doaj +2 more sources
Journal of Orthopaedic Translation, 2019 Background/Objectives: Decalcification of bone specimens is necessary for routine paraffin embedding and sectioning. Ethylenediaminetetraacetic acid (EDTA), a chelating agent for decalcification, maintains bone tissue integrity and histological features ...
Dick Ho Kiu Chow, Ling Qin, X Guo
exaly +3 more sources
Qualitative comparison of decalcifiers for mouse bone cryosections for subsequent biophotonic analysis [PDF]
Scientific ReportsBone tissue, with its complex structure, often necessitates decalcification of the hard tissue for ex vivo morphological studies. The choice of a suitable decalcification method plays a crucial role in preserving desired features and ensuring ...
Shibarjun Mandal +10 more
doaj +2 more sources
A rapid method for the preparation of an in vitro osteoporosis model of calf vertebrae: histological and biomechanical study [PDF]
Frontiers in Bioengineering and BiotechnologyBackgroundIn vitro biomechanical testing is crucial for the preclinical assessment of novel implant designs. Given the constraints of limited supply and high costs associated with human specimens, calf spines are frequently employed as surrogates for ...
Anli Shi +7 more
doaj +2 more sources
Enhancing Histological Techniques for Small Crustaceans: Evaluation of Fixation, Decalcification, and Enzymatic Digestion in Neocaridina Shrimp [PDF]
AnimalsHistological techniques are essential for studying small crustaceans’, such as Neocaridina shrimp, anatomy and physiology. However, their small size and rapid tissue autolysis present challenges for fixation and processing.
Rafał Karol Wild +5 more
doaj +2 more sources
Optimization of High-Frequency Ultrasound Imaging to Detect Incremental Changes in Mineral Content at the Cartilage–Bone Interface Ex Vivo [PDF]
Biomimetics(1) Background: Osteoarthritis is a degenerative disease of the whole joint marked by cartilage–bone interface (CBI) remodeling, but methods to monitor subtle changes in mineralization are lacking. We optimized a non-destructive ultrasound imaging method
Akshay Charan +2 more
doaj +2 more sources
Shipin yu jixie, 2022 Objective: To improve the extraction rate and product purity of Chinese soft-shelled turtle （pelodiscussinensis）dorsal collagen. Methods: The decalcification rate and collagen recovery rate were used as evaluation indicators to investigate the effects of
ZHAO Qiong-yu +4 more
doaj +1 more source
Shipin gongye ke-ji, 2022 In order to determine the technological conditions for decalcification of the carapace from the Chinese soft-shelled turtle(Pelodiscus sinensis), the decalcification conditions were optimized under ultrasonic assisted treatment with hydrochloric acid as ...
Qiongyu ZHAO +4 more
doaj +1 more source
Journal of Oral Biology and Craniofacial Research, 2022 The histopathological examination of mineralized tissues requires decalcification of teeth which is an essential and important step during tissue processing. In the present study we attempted to decalcify teeth using strong and weak acids and a chelating
Mohammad Imran Khan +5 more
doaj +1 more source