Results 121 to 130 of about 225,507 (258)

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

FEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn, Barbara Seroka, Ryszard Lazny, Zenon Lotowski, Thore Rohwerder   +4 more
wiley   +1 more source

3D visualization of in situ nanoscale dynamics in transmission electron microscopy via self-supervised deep learning [PDF]

BIO Web of Conferences
Craig Timothy, Kadu Ajinkya, Batenburg Kees, Bals Sara   +3 more
doaj   +1 more source

Constrained and unconstrained deep image prior optimization models with automatic regularization. [PDF]

Comput Optim Appl, 2023
Cascarano P, Franchini G, Kobler E, Porta F, Sebastiani A.   +4 more
europepmc   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Titan: Bringing the Deep Image Prior to Implicit Representations

ICASSP 2024 - 2024 IEEE International Conference on Acoustics, Speech and Signal Processing (ICASSP)
We study the interpolation capabilities of implicit neural representations (INRs) of images. In principle, INRs promise a number of advantages, such as continuous derivatives and arbitrary sampling, being freed from the restrictions of a raster grid. However, empirically, INRs have been observed to poorly interpolate between the pixels of the fit image;
Lorenzo Luzi, Daniel LeJeune, Ali Siahkoohi, Sina Alemohammad, Vishwanath Saragadam, Hossein Babaei, Naiming Liu, Zichao Wang 0001, Richard G. Baraniuk   +8 more
openaire   +2 more sources

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

RD-DIP: Rician denoising deep image prior

Neurocomputing
Rician denoising of magnetic resonance images (MRI) is a fundamental problem in medical image processing. Although variational methods can address Rician noise, the mathematical complexity of the underlying models makes them challenging to implement. Neural networks offer a compelling alternative but require extensive labelled datasets and are prone to
H. R. Iglesias-Goldaracena, Iván Ramírez, Emanuele Schiavi   +2 more
openaire   +3 more sources

Multi-prior physics-enhanced neural network enables pixel super-resolution and twin-image-free phase retrieval from single-shot hologram

Opto-Electronic Advances
Digital in-line holographic microscopy (DIHM) is a widely used interference technique for real-time reconstruction of living cells’ morphological information with large space-bandwidth product and compact setup.
Xuan Tian, Runze Li, Tong Peng, Yuge Xue, Junwei Min, Xing Li, Chen Bai, Baoli Yao   +7 more
doaj   +1 more source

Simultaneous Denoising of Dynamic PET Images Based on Deep Image Prior. [PDF]

J Digit Imaging, 2022
Yang CH, Huang HM.
europepmc   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source