Results 161 to 170 of about 17,182 (211)

Enzymatic deglycosylation of bovine rhodopsin

Experimental Eye Research, 1991
We have investigated the action of three endo N-acetylglucosaminidases on rhodopsin. The oligosaccharide chains of native and denatured opsin and rhodopsin, both solubilized and membrane-bound, were shown to be cleaved by endohexosaminidase H, endohexosaminidase F, and peptide-N-glycosidase F (PNGase F) as revealed by SDS-PAGE. These enzymes were shown
Edward L Kean, James J Plantner, J J Plantner   +2 more
exaly   +3 more sources

A chemical method for the deglycosylation of proteins

Archives of Biochemistry and Biophysics, 1987
A simple and rapid chemical method for the deglycosylation of glycoproteins has been developed. The method involves the incubation of protein with trifluoromethanesulfonic acid at 0 degrees C from 0.5 to 2 h followed by the neutralization of the acid with aqueous pyridine at -20 degrees C.
H T, Sojar, O P, Bahl
openaire   +2 more sources

Deglycosylation of glycoproteins by trifluoromethanesulfonic acid

Analytical Biochemistry, 1981
Abstract A new method for the chemical deglycosylation of glycoproteins is described. Treatment of fetuin with trifluoromethanesulfonic acid at 0 or 25°C results in rapid cleavage of peripheral sugars, slow loss of serine- and threonine-linked N-acetylgalactosamine, and retention of N-glycosidically linked N-acetylglucosamine. Amino acid analysis and
A S, Edge, C R, Faltynek, L, Hof, L E, Reichert, P, Weber   +4 more
openaire   +2 more sources

Hemoglobin binding to deglycosylated haptoglobin

Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1988
The carbohydrate portion of polymeric haptoglobin was gradually removed by exoglycosidases in order to investigate its role in complex formation between haptoglobin and hemoglobin. Total removal of sialic acid diminished the haptoglobin-hemoglobin complex formation 15%.
V, Kaartinen, I, Mononen
openaire   +2 more sources

Methods for Separation and Deglycosylation of Mucin Subunits

Analytical Biochemistry, 1995
Mucins (density 1.35-1.50 g/ml) prepared from human cervical, gastric, respiratory, and salivary secretions were reduced and alkylated to generate reduced mucin subunits. These subunits were separated into different populations analytically by using agarose gel electrophoresis and preparatively by using anion-exchange chromatography.
Thornton, David J., Howard, Marj, Devine, Peter L., Sheehan, John K.   +3 more
openaire   +5 more sources

The Charge Characterization of Native and Deglycosylated Thyrotropin

Endocrine Research, 1990
Chromatofocusing was used to characterize the charge microheterogeneity of crude pituitary, highly purified native bovine (b) and deglycosylated (dg) thyrotropin (TSH) preparations. Greater than 90% of crude pituitary TSH and native bTSH-I-1 bound to concanavalin-A (conA) columns while dgbTSH was excluded from the column.
B A, Keel, R L, Harms, S M, Amir
openaire   +2 more sources

A novel approach for chemically deglycosylating O-linked glycoproteins. The deglycosylation of submaxillary and respiratory mucins

Biochemistry, 1992
A new approach for removing O-glycosidically linked carbohydrate side chains from glycoproteins is described. Periodate oxidation of the C3 and C4 carbons in peptide-linked N-acetylgalactosamine (GalNAc) residues generates a dialdehyde product which, under mild alkaline conditions, undergoes a beta-elimination which releases carbohydrate and leaves an ...
T A, Gerken, R, Gupta, N, Jentoft
openaire   +2 more sources

Anhydrous hydrogen fluoride deglycosylates glycoproteins

Analytical Biochemistry, 1977
Abstract Exposure of glycoproteins to anhydrous hydrogen fluoride cleaves all the linkages of neutral and acidic sugars within 1 hr at 0°C while leaving peptide bonds and glycopeptide linkages of amino sugars intact. More severe treatment with anhydrous hydrogen fluoride (3 hr at 23°C) cleaves the O-glycosidic linkages of amino sugars, but peptide ...
A J, Mort, D T, Lamport
openaire   +2 more sources

Human Interferon-Beta: Effects of Deglycosylation

Journal of Interferon Research, 1982
Human fibroblast-derived interferon-beta (IFN-beta) labeled in vivo with 35S-methionine and purified to greater than 90% radiochemical purity has been used to study the effect of glycosidases on its biological activity and its molecular weight. Incubation of the radioactive interferon with the glycosidases causes a reduction in the estimated molecular ...
E, Knight, D, Fahey
openaire   +2 more sources

[Deglycosylation of glycoproteins].

Bioorganicheskaia khimiia, 1999
Glycosylation procedures and their application for the elucidation of glycoprotein structure and structure-function relations, as well as for the development of analytical systems for clinical practice are reviewed. For some common cases found in research practice, the choice of optimal deglycosylation methods is discussed. Current views on the primary
A A, Vinogradov, I A, Iamskov
openaire   +1 more source