Results 171 to 180 of about 17,182 (211)

Preparation of deglycosylated egg white avidin

Applied Biochemistry and Biotechnology, 1995
A simple procedure for the preparation of deglycosylated avidin is described. Commercially obtained avidin was treated with a mixed microbial culture. The cells were capable of growing on the oligosaccharide residues, but generally ignored the polypeptide portion of the egg white glycoprotein.
E A, Bayer, F, De Meester, T, Kulik, M, Wilchek   +3 more
openaire   +2 more sources

Dopamine transporter: deglycosylation with exo- and endoglycosidases

Brain Research, 1991
The dopamine transporter from rat caudate-putamen was photolabeled with [125I]DEEP as previously described. Treatment of photolabeled membranes with neuraminidase and N-glycanase reduced the molecular weight of the [125I]DEEP photolabeled dopamine transporter complex, whereas treatment with alpha-mannosidase had no effect.
R, Lew, D, Grigoriadis, A, Wilson, J W, Boja, R, Simantov, M J, Kuhar   +5 more
openaire   +2 more sources

Fungal sensitivity to and enzymatic deglycosylation of ginsenosides

Phytochemistry, 2012
A ginseng pathogen, Cylindrocarpon destructans, and five nonpathogens were tested for their sensitivity to a total ginsenoside fraction (T-GF), a protopanaxadiol-type ginsenoside fraction (PPD-GF) and a protopanaxatriol-type ginsenoside fraction (PPT-GF) from the roots of Panax ginseng C.A. Meyer.
Zhao, Xuesong, Gao, Juan, Song, Chengcheng, Fang, Qiang, Wang, Nan, Zhao, Tianjiao, Liu, Dongbo, Zhou, Yifa   +7 more
openaire   +5 more sources

Deglycosylation studies on tracheal mucin glycoproteins

Biochemistry, 1987
Following several model experiments, conditions were developed for optimal deglycosylation of tracheal mucin glycoproteins. Exposure of rigorously dried material to trifluoromethanesulfonic acid at 0 degree C for up to 8 h results in cleavage of essentially all fucose, galactose, and N-acetylglucosamine, about 80% of the N-acetylneuraminic acid (NeuNAc)
H D, Woodward, N J, Ringler, R, Selvakumar, I M, Simet, V P, Bhavanandan, E A, Davidson   +5 more
openaire   +2 more sources

The preparation of deglycosylated ricin by recombination of glycosidase-treated A- and B-chains: effects of deglycosylation on toxicity and in vivo distribution

Biochimica et Biophysica Acta (BBA) - General Subjects, 1987
Deglycosylation of ricin may be necessary to prevent the entrapment of antibody-ricin conjugates in vivo by cells of the reticuloendothelial system which have receptors that recognise the oligosaccharide side chains on the A- and B-chains of the toxin.
B M, Foxwell, D C, Blakey, A N, Brown, T A, Donovan, P E, Thorpe   +4 more
openaire   +2 more sources

Effect of deglycosylation on the stability of Aspergillus niger catalase

Archives of Biochemistry and Biophysics, 1981
Abstract Catalase from Aspergillus niger was isolated from a commercial preparation. The enzyme was found to be glycoprotein containing 9% neutral sugar and 3% hexosamine. A. niger catalase was markedly more stable than the beef liver enzyme to inactivation by heat, proteolysis, and glutaraldehyde modification.
B P, Wasserman, H O, Hultin
openaire   +2 more sources

Effects of Deglycosylation of Sodium Channels on Their Structure and Function

Biochemistry, 2004
Voltage-gated sodium channels are important membrane proteins underlying electrical signaling in the nervous and muscular systems. They undergo rapid conformational changes between closed resting, activated, and inactivated states. Approximately 30% of the mass of the sodium channel is carbohydrate, present as glycoconjugate chains, mostly composed of ...
Nora B, Cronin, Andrias, O'Reilly, Hervé, Duclohier, B A, Wallace   +3 more
openaire   +2 more sources

Deglycosylation of mAb by EndoS for Improved Molecular Imaging

Molecular Imaging and Biology, 2014
Monoclonal antibodies (mAbs) have been shown preclinically as reliable targeting moieties for antigen imaging using near-infrared fluorescence (NIRF) molecular imaging. However, crystallizable fragment-gamma receptor (FcγRs) expressed on immune cells also bind mAbs through defined epitopes on the constant fragment (Fc) of IgG.
Peng, Gao, Kenneth L, Pinkston, Nathaniel, Wilganowski, Holly, Robinson, Ali, Azhdarinia, Banghe, Zhu, Eva M, Sevick-Muraca, Barrett R, Harvey   +7 more
openaire   +2 more sources

[28] Enzymatic deglycosylation of glycoproteins

1987
Publisher Summary Deglycosylation of glycoproteins is important in elucidation of structure, function, and biosynthesis of biologically significant glycoproteins. Enzymatic deglycosylation can be brought about by exoglycosidases either used sequentially or in a mixture or by endoglycosidases.
Nageswara R. Thotakura, Om P. Bahl
openaire   +1 more source

Acidic natural deep eutectic solvents as dual solvents and catalysts for the solubilization and deglycosylation of soybean isoflavone extracts: Genistin as a model compound

Food Chemistry, 2023
Xianrui Liang, Sheng Fang
exaly