Results 181 to 190 of about 23,224 (220)

The Charge Characterization of Native and Deglycosylated Thyrotropin

Endocrine Research, 1990
Chromatofocusing was used to characterize the charge microheterogeneity of crude pituitary, highly purified native bovine (b) and deglycosylated (dg) thyrotropin (TSH) preparations. Greater than 90% of crude pituitary TSH and native bTSH-I-1 bound to concanavalin-A (conA) columns while dgbTSH was excluded from the column.
B A, Keel, R L, Harms, S M, Amir
openaire   +2 more sources

Preparation of deglycosylated egg white avidin

Applied Biochemistry and Biotechnology, 1995
A simple procedure for the preparation of deglycosylated avidin is described. Commercially obtained avidin was treated with a mixed microbial culture. The cells were capable of growing on the oligosaccharide residues, but generally ignored the polypeptide portion of the egg white glycoprotein.
E A, Bayer, F, De Meester, T, Kulik, M, Wilchek   +3 more
openaire   +2 more sources

Dopamine transporter: deglycosylation with exo- and endoglycosidases

Brain Research, 1991
The dopamine transporter from rat caudate-putamen was photolabeled with [125I]DEEP as previously described. Treatment of photolabeled membranes with neuraminidase and N-glycanase reduced the molecular weight of the [125I]DEEP photolabeled dopamine transporter complex, whereas treatment with alpha-mannosidase had no effect.
R, Lew, D, Grigoriadis, A, Wilson, J W, Boja, R, Simantov, M J, Kuhar   +5 more
openaire   +2 more sources

Anhydrous hydrogen fluoride deglycosylates glycoproteins

Analytical Biochemistry, 1977
Abstract Exposure of glycoproteins to anhydrous hydrogen fluoride cleaves all the linkages of neutral and acidic sugars within 1 hr at 0°C while leaving peptide bonds and glycopeptide linkages of amino sugars intact. More severe treatment with anhydrous hydrogen fluoride (3 hr at 23°C) cleaves the O-glycosidic linkages of amino sugars, but peptide ...
A J, Mort, D T, Lamport
openaire   +2 more sources

Deglycosylation studies on tracheal mucin glycoproteins

Biochemistry, 1987
Following several model experiments, conditions were developed for optimal deglycosylation of tracheal mucin glycoproteins. Exposure of rigorously dried material to trifluoromethanesulfonic acid at 0 degree C for up to 8 h results in cleavage of essentially all fucose, galactose, and N-acetylglucosamine, about 80% of the N-acetylneuraminic acid (NeuNAc)
H D, Woodward, N J, Ringler, R, Selvakumar, I M, Simet, V P, Bhavanandan, E A, Davidson   +5 more
openaire   +2 more sources

Fungal sensitivity to and enzymatic deglycosylation of ginsenosides

Phytochemistry, 2012
A ginseng pathogen, Cylindrocarpon destructans, and five nonpathogens were tested for their sensitivity to a total ginsenoside fraction (T-GF), a protopanaxadiol-type ginsenoside fraction (PPD-GF) and a protopanaxatriol-type ginsenoside fraction (PPT-GF) from the roots of Panax ginseng C.A. Meyer.
Zhao, Xuesong, Gao, Juan, Song, Chengcheng, Fang, Qiang, Wang, Nan, Zhao, Tianjiao, Liu, Dongbo, Zhou, Yifa   +7 more
openaire   +4 more sources

[Deglycosylation of glycoproteins].

Bioorganicheskaia khimiia, 1999
Glycosylation procedures and their application for the elucidation of glycoprotein structure and structure-function relations, as well as for the development of analytical systems for clinical practice are reviewed. For some common cases found in research practice, the choice of optimal deglycosylation methods is discussed. Current views on the primary
A A, Vinogradov, I A, Iamskov
openaire   +1 more source

The preparation of deglycosylated ricin by recombination of glycosidase-treated A- and B-chains: effects of deglycosylation on toxicity and in vivo distribution

Biochimica et Biophysica Acta (BBA) - General Subjects, 1987
Deglycosylation of ricin may be necessary to prevent the entrapment of antibody-ricin conjugates in vivo by cells of the reticuloendothelial system which have receptors that recognise the oligosaccharide side chains on the A- and B-chains of the toxin.
B M, Foxwell, D C, Blakey, A N, Brown, T A, Donovan, P E, Thorpe   +4 more
openaire   +2 more sources

Effects of Deglycosylation of Sodium Channels on Their Structure and Function

Biochemistry, 2004
Voltage-gated sodium channels are important membrane proteins underlying electrical signaling in the nervous and muscular systems. They undergo rapid conformational changes between closed resting, activated, and inactivated states. Approximately 30% of the mass of the sodium channel is carbohydrate, present as glycoconjugate chains, mostly composed of ...
Nora B, Cronin, Andrias, O'Reilly, Hervé, Duclohier, B A, Wallace   +3 more
openaire   +2 more sources

Deglycosylation of mAb by EndoS for Improved Molecular Imaging

Molecular Imaging and Biology, 2014
Monoclonal antibodies (mAbs) have been shown preclinically as reliable targeting moieties for antigen imaging using near-infrared fluorescence (NIRF) molecular imaging. However, crystallizable fragment-gamma receptor (FcγRs) expressed on immune cells also bind mAbs through defined epitopes on the constant fragment (Fc) of IgG.
Peng, Gao, Kenneth L, Pinkston, Nathaniel, Wilganowski, Holly, Robinson, Ali, Azhdarinia, Banghe, Zhu, Eva M, Sevick-Muraca, Barrett R, Harvey   +7 more
openaire   +2 more sources