Results 191 to 200 of about 23,224 (220)
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Effect of enzymatic deglycosylation on the regenerability of bovine rhodopsin
Experimental Eye Research, 1992The influence of the carbohydrate groups of rhodopsin on its ability to regenerate upon incubation with 11-cis retinaldehyde after photobleaching was examined. Rhodopsin was deglycosylated enzymatically with peptide-N-glycosidase F (PNGase F). Verification of deglycosylation was established by: (a) SDS-PAGE; (b) carbohydrate compositional analysis ...
A V, Prasad, J J, Plantner, E L, Kean
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Development and qualification of an antibody rapid deglycosylation method
Biologicals, 2012N-linked glycosylation can influence the biological activity and safety of an antibody as well as be a measure of the consistency of the production process. The released N-glycans is an important part of the development of a therapeutic antibody. The traditional method for N-glycan analysis requires complex and laborious sample preparation and lengthy ...
K Steven, Cook +2 more
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[28] Enzymatic deglycosylation of glycoproteins
1987Publisher Summary Deglycosylation of glycoproteins is important in elucidation of structure, function, and biosynthesis of biologically significant glycoproteins. Enzymatic deglycosylation can be brought about by exoglycosidases either used sequentially or in a mixture or by endoglycosidases.
Nageswara R. Thotakura, Om P. Bahl
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Deglycosylation of chondroitin sulfate proteoglycan and derived peptides
Biochemistry, 1990In order to define the domain structure of proteoglycans as well as identify primary amino acid sequences specific for attachment of the various carbohydrate substituents, reliable techniques for deglycosylating proteoglycans are required. In this study, deglycosylation of cartilage chondroitin sulfate proteoglycan (CSPG) with minimal core protein ...
S C, Campbell +2 more
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Effect of deglycosylation on the stability of Aspergillus niger catalase
Archives of Biochemistry and Biophysics, 1981Abstract Catalase from Aspergillus niger was isolated from a commercial preparation. The enzyme was found to be glycoprotein containing 9% neutral sugar and 3% hexosamine. A. niger catalase was markedly more stable than the beef liver enzyme to inactivation by heat, proteolysis, and glutaraldehyde modification.
B P, Wasserman, H O, Hultin
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Active deglycosylated mammalian γ‐glutamyl transpeptidase
The FASEB Journal, 1994γ‐Glutamyl transpeptidase, a highly glycosylated heterodimeric enzyme that is usually attached to the external surface of cell membranes, is of major importance in the metabolism of glutathione. The enzyme, which has been isolated from many animal sources, contains a large amount of carbohydrate, which is linked to both protein ...
T K, Smith, A, Meister
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Enzymatic deglycosylation of human thyroglobulin: fluorescence studies
Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1988The interaction between the carbohydrate and the amino acid residues in human thyroglobulin has been studied. Previous reports showed that the removal of the two terminal carbohydrates of the complex chains leads to an increase in thyroglobulin binding to thyroid membranes.
Grimaldi S +6 more
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The toxicity of chemically deglycosylated ricin A-chain in mice
International Journal of Immunopharmacology, 1992Tumor-reactive antibodies coupled to ricin or its A-chain (immunotoxins) have been used in rodents and humans to treat a variety of neoplastic diseases. Side-effects of such treatment include hepatotoxicity, vascular leak syndrome, myalgia and low grade fever.
A M, Soler-Rodriguez +3 more
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Hydrogen Fluoride Deglycosylation of Glycoproteins
Cold Spring Harbor Protocols, 2006David, Oxley +2 more
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