A De Novo Designed Metalloprotein Displays Variable Thermal Stability and Binding Stoichiometry with Transition Metal Ions. [PDF]
ChembiochemA de novo designed single‐chain coiled coil protein is unfolded in apo‐state but folds up upon addition of divalent transition metal ions Co(II), Ni(II), Cu(II), and Zn(II). The resulting metal‐bound complexes exhibit distinct thermal stability and metal stoichiometry, making this protein a promising starting point for the design of fully metal ...
Rooijakkers B +4 more
europepmc +2 more sources
Evaluating zero-shot prediction of monomeric protein design success by AlphaFold, ESMFold, and ProteinMPNN. [PDF]
Protein SciAbstract De novo protein design has enabled the creation of proteins with diverse functionalities that are not found in nature. Despite recent advances, experimental success rates remain inconsistent and context‐dependent, posing a bottleneck for broader applications of de novo design.
Garcia M, Dixit SM, Rocklin GJ.
europepmc +2 more sources
Relative affinity constants by electrospray ionization and Fourier transform ion cyclotron resonance mass spectrometry: calmodulin binding to peptide analogs of myosin light chain kinase [PDF]
, 2003 Synthetic RS20 peptide and a set of its point-mutated peptide analogs have been used to analyze the interactions between calmodulin (CaM) and the CaM-binding sequence of smooth-muscle myosin light chain kinase both in the presence and the absence of Ca2+.
Nousiainen, Marjaana +3 more
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Dambovita - 50 km between good quality and ecological disaster [PDF]
Cinq Continents, 2011 Dambovita - 50 km tra buona qualità e disastro ecologico. Dambovita è il principale corso d'acqua che attraversa Bucarest. Quindi, è l'acqua collettore delle acque reflue, industriali e pluviale generati all'interno della città, arrivando prima ...
DUMITRU MORCOTET +2 more
doaj
A real-time all-atom structural search engine for proteins. [PDF]
PLoS Computational Biology, 2014 Protein designers use a wide variety of software tools for de novo design, yet their repertoire still lacks a fast and interactive all-atom search engine.
Gabriel Gonzalez +2 more
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Domingo de Ramos, dalla violenza urbana alla solidarietà marginale
Confluenze, 2016 The article aims to analyze the poetry of Domingo de Ramos (Peru 1960) through themes like violence, urban decay and migration and presents the translation of an unpublished poem.
Riccardo Badini
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Disegno e documentazione per il restauro: un impegno interdisciplinare
Disegnare con, 2012 Una buona indagine sul costruito, che veda il rilievo come suo strumento principale, mirerà a favorire un approccio basato sull'apporto interdisciplinare del disegno e di altre metodologie analitiche, fisico-chimiche, strutturali, archeologiche e ...
Giovanni Carbonara
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Influence of molecular structure on the antimicrobial function of phenylenevinylene conjugated oligoelectrolytes. [PDF]
, 2016 Conjugated oligoelectrolytes (COEs) with phenylenevinylene (PV) repeat units are known to spontaneously intercalate into cell membranes. Twelve COEs, including seven structures reported here for the first time, were investigated for the relationship ...
Bazan, Guillermo C +5 more
core +2 more sources
Self-assembling dipeptide antibacterial nanostructures with membrane disrupting activity. [PDF]
, 2017 Peptide-based supramolecular assemblies are a promising class of nanomaterials with important biomedical applications, specifically in drug delivery and tissue regeneration.
Adler-Abramovich, Lihi +10 more
core +3 more sources
Archeomatica, 2017 The goal of this study is to describe the use of GIS to analyze and organize the degradation patterns of natural and artificial stones, according to the recommendations of the "Ente Italiano di Unificazione”, contained in the lexicon "UNI 11182/2006 ...
Francesco Miraglia
doaj +1 more source