FEBS Open Bio, EarlyView.Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Assessment of transformer winding displacement faults by frequency response analysis
, 2010 According to the statistics of transformer accidents from 1995 to 1998, nearly 50% of the accidents were caused by short circuit, and the number is still increasing.
Muhamad, Shahriman
core
Design study of a high temperature superconducting generator with YBCO windings
, 2009 This paper considers the potential merits of using a HTS rotor winding in a low-speed high-torque synchronous generator and the possibility of building a small-scale demonstrator to investigate the practicality of building such a full-size machine ...
Lukasik, B. +3 more
core +1 more source
FEBS Open Bio, EarlyView.Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata +13 more
wiley +1 more source
Indicator Selection for Life Prediction of Polyimide Enameled Wire for Aviation Generators and Method for Establishing Life Curve-Based on Bayesian Nonlinear Regression. [PDF]
Polymers (Basel)Wang Z, Liu Y, Li T, Zhu P, Niu G, Du H.
europepmc +1 more source
RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells
FEBS Open Bio, EarlyView.RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour +2 more
wiley +1 more source
Bio-Inspired Bidirectional Winding Origami With Programmable Modular Reconfigurability and Scalability. [PDF]
Adv Sci (Weinh)Zhang W +5 more
europepmc +1 more source
FEBS Open Bio, EarlyView.In normal (nontolerant) cells, CD14 is crucial for both LPS uptake and LPS signaling. In LPS‐tolerant cells, in which LPS‐induced TNF‐α and IFN‐β production is suppressed, there is a dramatic increase in surface CD14 expression. The overexpressed CD14 in LPS‐tolerant cells is responsible for the enhanced LPS uptake without inducing pro‐inflammatory ...
Saeka Nishihara +3 more
wiley +1 more source
The Influence of Fiber Tension and Filament Winding Patterns on the Strength of Thin-Walled Fiber-Reinforced Polymer Composite Tubes. [PDF]
Polymers (Basel)Paczkowska K +2 more
europepmc +1 more source