Results 151 to 160 of about 77,339,877 (193)
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Acta Oceanologica Sinica, 2019
The cDNA of the delta-12 fatty acid desaturase gene, IgFAD2, was cloned from the marine microalgae Isochrysis galbana, a species capable of producing docosahexaenoic acid. Sequence analysis indicated that the open reading frame measured a length of 1 158 bp and encoded 386 amino acids with a predicted molecular weight of 42.8 kDa and an isoelectric ...
Xiaotian Han +3 more
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The cDNA of the delta-12 fatty acid desaturase gene, IgFAD2, was cloned from the marine microalgae Isochrysis galbana, a species capable of producing docosahexaenoic acid. Sequence analysis indicated that the open reading frame measured a length of 1 158 bp and encoded 386 amino acids with a predicted molecular weight of 42.8 kDa and an isoelectric ...
Xiaotian Han +3 more
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Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1990
The cockroach, Periplaneta americana, can convert oleic acid (18:1(n - 9], to linoleic acid, (18:2(n - 6], by a microsomal delta 12 desaturase. Most of the desaturase activity was present in the fat body tissue, with lower activity in the epidermis and no detectable activity in the thorax or gut tissue.
C E, Borgeson +2 more
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The cockroach, Periplaneta americana, can convert oleic acid (18:1(n - 9], to linoleic acid, (18:2(n - 6], by a microsomal delta 12 desaturase. Most of the desaturase activity was present in the fat body tissue, with lower activity in the epidermis and no detectable activity in the thorax or gut tissue.
C E, Borgeson +2 more
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Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1996
The Fad12 mutant of Synechocystis sp. PCC 6803 has a defect in the desA gene for delta 12 acyl-lipid desaturase. We identified a change in the nucleotide sequence of the structural gene for the desaturase, in which a leucine codon has been converted to a stop codon. Western blot analysis revealed that the delta 12 acyl-lipid desaturase was localized in
Z, Gombos +4 more
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The Fad12 mutant of Synechocystis sp. PCC 6803 has a defect in the desA gene for delta 12 acyl-lipid desaturase. We identified a change in the nucleotide sequence of the structural gene for the desaturase, in which a leucine codon has been converted to a stop codon. Western blot analysis revealed that the delta 12 acyl-lipid desaturase was localized in
Z, Gombos +4 more
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Analytical Biochemistry, 2002
Transmembrane domains of the acyl-coenzyme A and acyl phosphatidylcholine-utilizing desaturases may control interactions with electron transport domains, be involved in substrate specificity and/or serve as a structural foundation for the enzyme.
Robert E, Minto +3 more
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Transmembrane domains of the acyl-coenzyme A and acyl phosphatidylcholine-utilizing desaturases may control interactions with electron transport domains, be involved in substrate specificity and/or serve as a structural foundation for the enzyme.
Robert E, Minto +3 more
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Molecular Breeding, 2011
A full-length cDNA clone of OsFAD2, which encodes a Δ-12 fatty acid desaturase, the key enzyme for the conversion of oleic acid (18:1) into linoleic acid (18:2), was isolated from rice (Oryza sativa ssp. japonica) leaves. The deduced amino acid sequence of OsFAD2 displayed three histidine boxes characteristic of all membrane-bound desaturases, and ...
Jinlei Shi +5 more
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A full-length cDNA clone of OsFAD2, which encodes a Δ-12 fatty acid desaturase, the key enzyme for the conversion of oleic acid (18:1) into linoleic acid (18:2), was isolated from rice (Oryza sativa ssp. japonica) leaves. The deduced amino acid sequence of OsFAD2 displayed three histidine boxes characteristic of all membrane-bound desaturases, and ...
Jinlei Shi +5 more
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Heterologous Expression of Mucor rouxii Δ12-Desaturase Gene in Saccharomyces cerevisiae,
Biochemical and Biophysical Research Communications, 1999In this study we present the cloning and functional characterization of a gene whose product is responsible for Delta(12)-desaturase activity and is involved in the metabolic pathway of gamma-linolenic acid (GLA) synthesis of Mucor rouxii. A cDNA encoding for Delta(12)-desaturase of M.
S, Passorn +4 more
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Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 2001
Two overlapping genomic clones spanning 16.5 kb of cotton DNA were found to encompass a Delta-12 fatty acid desaturase (FAD2-3) gene. A partial FAD2-3 cDNA clone was also analyzed. The FAD2-3 gene has one large intron of 2967 bp entirely within its 5'-untranslated region, only 12 bp upstream from the ATG initiation codon.
I L, Pirtle +5 more
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Two overlapping genomic clones spanning 16.5 kb of cotton DNA were found to encompass a Delta-12 fatty acid desaturase (FAD2-3) gene. A partial FAD2-3 cDNA clone was also analyzed. The FAD2-3 gene has one large intron of 2967 bp entirely within its 5'-untranslated region, only 12 bp upstream from the ATG initiation codon.
I L, Pirtle +5 more
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Archives of Biochemistry and Biophysics, 2000
We have cloned a Caenorhabditis elegans cDNA encoding a Delta12 fatty acid desaturase and demonstrated its activity by heterologous expression in Saccharomyces cerevisiae. The predicted protein is highly homologous both to the cloned plant genes with similar function and to the published sequence of the C. elegans omega-3 fatty acid desaturase.
M M, Peyou-Ndi, J L, Watts, J, Browse
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We have cloned a Caenorhabditis elegans cDNA encoding a Delta12 fatty acid desaturase and demonstrated its activity by heterologous expression in Saccharomyces cerevisiae. The predicted protein is highly homologous both to the cloned plant genes with similar function and to the published sequence of the C. elegans omega-3 fatty acid desaturase.
M M, Peyou-Ndi, J L, Watts, J, Browse
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Archives of Biochemistry and Biophysics, 1990
A novel delta 12-desaturase from animals, which converts oleic acid (18:1n-9) to linoleic acid (18:2n-6), was characterized in the house cricket, Acheta domesticus. The delta 12-desaturase product, linoleic acid, was determined by silver nitrate thin-layer chromatography, radio-gas-liquid chromatography and radio-high-performance liquid chromatography ...
C, Cripps +3 more
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A novel delta 12-desaturase from animals, which converts oleic acid (18:1n-9) to linoleic acid (18:2n-6), was characterized in the house cricket, Acheta domesticus. The delta 12-desaturase product, linoleic acid, was determined by silver nitrate thin-layer chromatography, radio-gas-liquid chromatography and radio-high-performance liquid chromatography ...
C, Cripps +3 more
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Crop Science, 2008
In an effort to better understand the mechanisms that regulate oil accumulation and packaging in seeds, transgenic cotton lines were generated using a Brassica napus nonfunctional delta‐12 fatty acid desaturase (FAD2) gene under control of the phaseolin promoter.
Kent D. Chapman +11 more
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In an effort to better understand the mechanisms that regulate oil accumulation and packaging in seeds, transgenic cotton lines were generated using a Brassica napus nonfunctional delta‐12 fatty acid desaturase (FAD2) gene under control of the phaseolin promoter.
Kent D. Chapman +11 more
openaire +1 more source